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Study Information
Study Attribute Value
CEBS Accession Number: 002-01793-0002-0000-4
Chemical Name: C.I. Disperse blue 1
CASRN: 2475-45-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 776658_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
Induced Rat Liver S9  1_S9  Questionable 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 07/10/1986    Harvest Time: 10 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Ethanol 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Disperse blue 1 
8         
 100 5 0.050 4.0 0 0.000 0.0 5 0.050 4.0 0 0.000 0.0 
9         
 100 7 0.070 7.0 4 0.040 4.0 3 0.030 3.0 0 0.000 0.0 
10         
 100 10 0.100 10.0 5 0.050 5.0 4 0.040 4.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.25      
 100 17 0.170 12.0 7 0.070 6.0 10 0.100 8.0 0 0.000 0.0
Mitomycin-C 
 0.75      
 25 18 0.720 44.0 5 0.200 20.0 13 0.520 44.0 0 0.000 0.0
Trend: 2.592 1.786 1.499  
Probability: 0.005 0.037 0.067  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/10/1986    Harvest Time: 12 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Ethanol 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: C.I. Disperse blue 1 
8         
 100 8 0.080 7.0 1 0.010 1.0 6 0.060 5.0 1 0.010 1.0 
9         
 100 16 0.160 11.0 6 0.060 5.0 7 0.070 4.0 3 0.030 3.0 
10         
 100 9 0.090 8.0 4 0.040 4.0 4 0.040 3.0 1 0.010 1.0
Positive Control: Cyclophosphamide 
7.5       
 100 12 0.120 12.0 8 0.080 8.0 4 0.040 4.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 20 0.800 52.0 15 0.600 40.0 5 0.200 16.0 0 0.000 0.0
Trend: 1.956 1.332 1.057  
Probability: 0.025 0.091 0.145  


Trial #:2   Activation: No Activation    Date: 06/26/1986    Harvest Time: 10 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0
Ethanol 
0         
 200 3 0.015 1.5 2 0.010 1.0 0 0.000 0.0 1 0.005 0.5
Test Chemical: C.I. Disperse blue 1 
5         
 200 8 0.040 4.0 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 
7.5       
 200 11 0.055 5.5 5 0.025 2.5 4 0.020 2.0 2 0.010 1.0 
10         
 200 25 0.125 11.0 14 0.070 6.0 2 0.010 1.0 9 0.045 4.5
Positive Control: Mitomycin-C 
 0.25      
 200 16 0.080 7.0 9 0.045 3.5 6 0.030 3.0 1 0.005 0.5
Mitomycin-C 
 0.75      
 25 17 0.680 52.0 8 0.320 28.0 9 0.360 28.0 0 0.000 0.0
Trend: 4.084 2.855 1.627  
Probability: 0.000 0.002 0.052  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/26/1986    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 11 0.055 4.5 8 0.040 3.0 0 0.000 0.0 3 0.015 1.5
Ethanol 
0         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: C.I. Disperse blue 1 
5         
 200 4 0.020 2.0 3 0.015 1.5 1 0.005 0.5 0 0.000 0.0 
7.5       
 200 8 0.040 4.0 1 0.005 0.5 5 0.025 2.5 2 0.010 1.0 
10         
 200 21 0.105 9.0 7 0.035 3.5 10 0.050 3.5 4 0.020 2.0
Positive Control: Cyclophosphamide 
7.5       
 200 23 0.115 9.0 7 0.035 3.5 14 0.070 5.5 2 0.010 1.0
Cyclophosphamide 
37.5       
 25 14 0.560 32.0 8 0.320 28.0 6 0.240 12.0 0 0.000 0.0
Trend: 3.525 1.502 2.222  
Probability: 0.000 0.067 0.013