Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01793-0009-0000-1
Chemical Name:	C.I. Disperse blue 1
CASRN:	2475-45-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	334618
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	96	40	15	14
			98	90	41	14
			87	109	29	11
			91	105	42	15
Test Chemical	C.I. Disperse blue 1	10	92	51	107	39	41*
			92	65	119	43	41*
		20	81	47	120	50	50*
			84	61	125	50	50*
		40	70	37	141	67	65*
			79	37	150	63	65*
		80	70	19	141	67	78*
			62	26	164	88	78*
		160	30	5	112	125	118*
			42	7	140	110	118*
Positive Control	Methyl Methane Sulfonate	15	40	33	152	126	138*
Positive Control			39	30	173	150	138*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	96	65	29	25
			73	104	43	20
			67	112	40	20
			56	88	53	31
Test Chemical	C.I. Disperse blue 1	2.5	73	87	80	37	39
			68	73	85	42	39
		5	65#	39	131	67	75*
			63	55	156	82	75*
		10	56	41	128	76	79*
			57	29	141	83	79*
		20	52	39	110	71	64*
			60	47	103	57	64*
		40	45	26	141	104	95*
			57	30	146	85	95*
		80	39	22	96	82	112*
			44#	19	188	142	112*
Positive Control	Methyl Methane Sulfonate	15	28	17	139	164	183*
Positive Control			34	23	208	203	183*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination