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Study Information
Study Attribute Value
CEBS Accession Number: 002-01793-0009-0000-1
Chemical Name: C.I. Disperse blue 1
CASRN: 2475-45-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 334618
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 96 40 15 14
    98 90 41 14
    87 109 29 11
    91 105 42 15
Test Chemical C.I. Disperse blue 1 
10         
 92 51 107 39 41*
    92 65 119 43
  
20         
 81 47 120 50 50*
    84 61 125 50
  
40         
 70 37 141 67 65*
    79 37 150 63
  
80         
 70 19 141 67 78*
    62 26 164 88
  
160         
 30 5 112 125 118*
    42 7 140 110
Positive Control Methyl Methane Sulfonate 
15         
 40 33 152 126 138*
    39 30 173 150

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 96 65 29 25
    73 104 43 20
    67 112 40 20
    56 88 53 31
Test Chemical C.I. Disperse blue 1 
2.5       
 73 87 80 37 39
    68 73 85 42
  
5         
 65# 39 131 67 75*
    63 55 156 82
  
10         
 56 41 128 76 79*
    57 29 141 83
  
20         
 52 39 110 71 64*
    60 47 103 57
  
40         
 45 26 141 104 95*
    57 30 146 85
  
80         
 39 22 96 82 112*
    44# 19 188 142
Positive Control Methyl Methane Sulfonate 
15         
 28 17 139 164 183*
    34 23 208 203

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination