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Study Information
Study Attribute Value
CEBS Accession Number: 002-01796-0011-0000-7
Chemical Name: C.I. Disperse yellow 3
CASRN: 2832-40-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 571100
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 109 65 24 25
    100 112 76 25
    99 93 68 23
    83 85 65 26
Test Chemical C.I. Disperse yellow 3 
2.5       
 90 110 65 24 26
    85 103 71 28
  
5         
 119 126 69 19 19
    83 127 47 19
  
10         
 84 95 113 45 34
    85 124 59 23
  
20         
 97 97 75 26 27
    83 98 68 27
  
40         
 107 79 90 28 24
    84 90 50 20
Positive Control Methyl Methane Sulfonate 
15         
 49 38 118 80 90*
    50 35 151 101
Methyl Methane Sulfonate 
250         
 90 110 335 124 137*
    70 84 315 150

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 102 111 49 49
    62 104 102 55
    76 95 98 43
    75 99 108 48
Test Chemical C.I. Disperse yellow 3 
2.5       
 80 123 130 54 47
    117 142 136 39
  
5         
 89 117 127 48 45
    67 110 84 42
  
10         
 68 98 101 50 44
    75 112 87 39
  
20         
 73 100 113 52 51
    80 93 121 51
  
40         
 83 77 149 60 55
    82 76 122 50
Positive Control Methyl Methane Sulfonate 
15         
 35 28 186 178 177*
    36 31 191 176
Methyl Methane Sulfonate 
250         
 53 90 275 173 182*
    62 78 354 192

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 77 122 59 51
    76 86 119 52
    86 116 122 47
    73 120 100 45
Test Chemical C.I. Disperse yellow 3 
2.5       
 58 67 90 52 59
    68 82 135 67
  
5         
 56 56 113 67 68
    60 47 124 69
  
10         
 78 45 200 85 80*
    66 33 146 74
  
20         
 70 33 274 131 116*
    76 25 230 100
Positive Control 3-Methylcholanthrene 
2.5       
 43 32 605 471 461*
    40 32 542 452

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 98 194 76 72
    92 93 195 71
    95 91 221 77
    97 118 188 64
Test Chemical C.I. Disperse yellow 3 
5         
 93 88 223 80 74
    100 95 206 69
  
10         
 59 52 168 95 93
    87 71 235 90
  
20         
 75 38 276 123 105
    89 53 234 87
  
30         
 90 36 301 111 114*
    70 31 247 117
  
40         
 79 23 339 143 132*
    100 22 362 120
Positive Control 3-Methylcholanthrene 
2.5       
 61 34 696 381 399*
    53 32 665 417

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination