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Study Information
Study Attribute Value
CEBS Accession Number: 002-01812-0011-0000-6
Chemical Name: C.I. Solvent yellow 14
CASRN: 842-07-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 841852
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 113 100 126 37 42
    96 101 165 58
    95 98 111 39
    75 101 75 33
Test Chemical C.I. Solvent yellow 14 
3.125     
 121r 87 147 40
    138r 97 201 48
  
6.25      
 86 93 99 39 45
    108 118 170 52
  
12.5       
 79 59 116 49 49
    70 54 104 50
  
25         
 48 6 1330 927 985*
    37 6 1157 1042
  
50         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 25 182 136 133*
    37 25 146 131
Methyl Methane Sulfonate 
250         
 82 124 223 90 85*
    66 80 156 79

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 68 85 115 56 48
    76 107 114 50
    78 102 98 42
    90 106 120 45
Test Chemical C.I. Solvent yellow 14 
1         
 70 84 108 52 54
    67 83 113 56
  
2         
 88 100 109 41 44
    77 87 109 47
  
4         
 106 92 139 44 49
    86 67 141 55
  
8         
 88 80 141 54 46
    73 89 82 38
  
16         
 74 34 133 60 57
    71 26 114 53
Positive Control Methyl Methane Sulfonate 
15         
 41 31 218 176 172*
    52 32 261 167
Methyl Methane Sulfonate 
250         
 74 73 427 193 187*
    73 69 399 182

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 52 81 126 81 63
    61 104 107 58
    66 97 112 57
    79 118 130 55
Test Chemical C.I. Solvent yellow 14 
1         
 75 119 164 73 61
    82 109 123 50
  
2         
 60 103 124 69 64
    82 71 145 59
  
4         
 52 33 141 91 105*
    47 43 168 120
  
8         
 44 16 204 156 142*
    44 30 170 128
  
16         
 56 30 171 101 103*
    58 31 181 105
Positive Control 3-Methylcholanthrene 
2.5       
 7r 2 326 1590 653*
    20 5 385 653

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 58 94 113 65 53
    57 92 114 67
    84 98 115 46
    82 115 86 35
Test Chemical C.I. Solvent yellow 14 
1         
 63 87 120 64 58
    72 91 113 52
  
2         
 57 73 102 59 72
    60 72 152 85
  
4         
 51 37 134 88 79
    56 36 118 70
  
8         
 24 11 143 199 175*
    38 18 172 152
  
16         
 52 28 101 64 69
    62 32 137 74
Positive Control 3-Methylcholanthrene 
2.5       
 15 4 177 398 418*
    18 5 234 437

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 96 117 86 30 33
    97 105 108 37
    90 88 73 27
    90 90 103 38
Test Chemical C.I. Solvent yellow 14 
2         
 65 83 89 46 41
    67 82 73 36
  
4         
 64 52 107 55 49
    84 73 109 44
  
8         
 63 28 144 77 85*
    47 27 133 94
  
12         
 38 9 124 110 91*
    39 13 86 73
  
16         
 33 9 85 86 75*
    24 6 45 63
Positive Control 3-Methylcholanthrene 
2.5       
 14 2 262 616 566*
    13 3 193 515

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 101 103 135 45 49
    92 103 145 52
    95 97 154 54
    97 96 132 45
Test Chemical C.I. Solvent yellow 14 
1         
 105 87 155 49 56
    94 77 175 62
  
2         
 84 72 176 70 63
    96 70 163 57
  
4         
 83 39 134 54 66
    86 42 199 77
  
8         
 68 16 178 87 79*
    72 19 151 70
  
16         
 54 9 148 92 96*
    47 6 142 101
Positive Control 3-Methylcholanthrene 
2.5       
 54 21 603 370 349*
    58 28 570 328

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination