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Study Information
Study Attribute Value
CEBS Accession Number: 002-01814-0002-0000-8
Chemical Name: C.I. Vat brown 3
CASRN: 131-92-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 728480_CA
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 08/09/1988    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
500         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1000         
 200 5 0.025 2.5 0 0.000 0.0 4 0.020 2.0 1 0.005 1.0 
1600         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 39 0.195 15.0 18 0.090 7.5 21 0.105 9.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 22 0.440 36.0 9 0.180 14.0 13 0.260 26.0 0 0.000 0.0
Trend: -0.579 0.000 -0.814  
Probability: 0.719 0.000 0.792  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 08/09/1988    Harvest Time: 13 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
1000         
 200 16 0.080 6.0 2 0.010 1.0 13 0.065 5.0 1 0.005 1.0 
1600         
 200 5 0.025 2.5 1 0.005 0.5 4 0.020 2.0 0 0.000 0.0 
2500         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 22 0.110 10.5 6 0.030 3.0 16 0.080 7.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 21 0.420 36.0 7 0.140 12.0 14 0.280 24.0 0 0.000 0.0
Trend: 0.180 -0.255 0.290  
Probability: 0.429 0.601 0.386  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/20/1988    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
1000         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1600         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
2500         
 200 11 0.055 4.0 1 0.005 0.5 8 0.040 3.0 2 0.010 2.0
Positive Control: Cyclophosphamide 
2.5       
 200 16 0.080 7.0 0 0.000 0.0 16 0.080 7.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 19 0.380 32.0 3 0.060 6.0 16 0.320 28.0 0 0.000 0.0
Trend: 3.109 1.333 2.540  
Probability: 0.001 0.091 0.006