Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-01814-0002-0000-8
Chemical Name: C.I. Vat brown 3
CASRN: 131-92-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 728480_CA
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 08/09/1988    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
500         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1000         
 200 5 0.025 2.5 0 0.000 0.0 4 0.020 2.0 1 0.005 1.0 
1600         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 39 0.195 15.0 18 0.090 7.5 21 0.105 9.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 22 0.440 36.0 9 0.180 14.0 13 0.260 26.0 0 0.000 0.0
Trend: -0.579 0.000 -0.814  
Probability: 0.719 0.000 0.792  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 08/09/1988    Harvest Time: 13 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
1000         
 200 16 0.080 6.0 2 0.010 1.0 13 0.065 5.0 1 0.005 1.0 
1600         
 200 5 0.025 2.5 1 0.005 0.5 4 0.020 2.0 0 0.000 0.0 
2500         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 22 0.110 10.5 6 0.030 3.0 16 0.080 7.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 21 0.420 36.0 7 0.140 12.0 14 0.280 24.0 0 0.000 0.0
Trend: 0.180 -0.255 0.290  
Probability: 0.429 0.601 0.386  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/20/1988    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: C.I. Vat brown 3 
1000         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1600         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
2500         
 200 11 0.055 4.0 1 0.005 0.5 8 0.040 3.0 2 0.010 2.0
Positive Control: Cyclophosphamide 
2.5       
 200 16 0.080 7.0 0 0.000 0.0 16 0.080 7.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 19 0.380 32.0 3 0.060 6.0 16 0.320 28.0 0 0.000 0.0
Trend: 3.109 1.333 2.540  
Probability: 0.001 0.091 0.006