Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01816-0005-0000-3
Chemical Name:	beta-Cadinene
CASRN:	523-47-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	138289
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	76	69	47	21	24
			93	118	68	25
			104	107	88	28
			99	105	62	21
Test Chemical	beta-Cadinene	0.005	88	89	81	31	22
			106	101	70	22
			93	105	37	13
		0.01	81	87	56	23	26
			99	136	77	26
			86	94	74	29
		0.02	115	95	90	26	26
			73	90	72	33
			98#	93	54	18
		0.03	90	78	54	20	28
			84	57	50	20
			77	50	102	44
		0.04	106	49	70	22	39*
			64	6	97	50
			81	29	105	43
		0.05


Positive Control	Ethyl Methane Sulfonate	500	12	5	562	1606	1269*
			8r	3	496	2111
			22#	13	615	932

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	98	89	72	24	22
			98	114	72	25
			112	99	63	19
			104	99	68	22
Test Chemical	beta-Cadinene	0.005	103	80	56	18	21
			84	86	54	21
			93#	85	67.5	24
		0.01	112#	91	63	19	17
			110	98	44	13
			103	95	60	20
		0.02	131r	107	56	14	24
			76	80	69	30
			89	79	49	18
		0.03	81	75	46	19	19
			97	70	61	21
			100#	88	55	18
		0.04	92	48	65	24	22
			95	41	39	14
			72	24	63	29
		0.05


Positive Control	Ethyl Methane Sulfonate	250	69#	42	651	315	362*
			80	34	828	344
			68	33	867	426

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	63#	104	54	28	35
			80	106	96	40
			85	91	96	38
Test Chemical	beta-Cadinene	0.02	87	128	29	11	43
			56	81	88	53
			47	55	92	65
		0.03	66	92	41	21	29
			70#	96	96	46
			88	108	58	22
		0.04	47	57	61	43	32
			90	92	58	22
			78	116	72	31
		0.05	78	74	147	63	52
			84#	75	138	55
			59	73	68	39
		0.06	85	35	98	39	44
			66	50	96	48
			40	46	54	45
		0.08	48	3	177	124	96*
			46	4	153	111
			73	14	114	52
Positive Control	3-Methylcholanthrene	5	25	7	284	386	372*
			23	2	276	400
			18#	3	178	330

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	66#	83	131	66	62
			92	108	155	56
			82	112	156	63
			84	97	151	60
Test Chemical	beta-Cadinene	0.01	89#	94	228	86	68
			96	90	181	63
			95	104	154	54
		0.02	97#	75	160.5	55	60
			86	105	166	64
			86	95	156	61
		0.04					77
			91	24	187	69
			83	63	210	84
		0.05


Positive Control	3-Methylcholanthrene	2.5	49	23	597	408	373*
			64	33	634	333
			54	27	616	378

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	80	94	129	54	62
			74	104	125	56
			80	98	145	60
			66	104	156	79
Test Chemical	beta-Cadinene	0.01	72	84	141	66	64
			77	90	144	62	64
		0.02	96#	91	205.5	71	55
			82	119	115	47
			80	118	109	46
		0.03	60	24	113	63	59
			79	92	156	66
			75	131	107	48
		0.04	85	76	165	65	68
			62	41	129	70
			64	29	133	69
		0.05	89	51	105	39	59
			93	107	144	52
			81	89	207	85
		0.06


Positive Control	3-Methylcholanthrene	2.5	60	26	474	262	278*
			72	52	509	237
			47	23	472	335

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination