Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01821-0010-0000-5
Chemical Name:	Calcium cyanamide
CASRN:	156-62-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	430294
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	47r	91	101	72	57
			57	102	104	61
			55	98	87	53
			42r	74	102	81
Test Chemical	Calcium cyanamide	25	85	147	84	33	36
			75	120	86	38	36
		50	59	106	76	43	46
			71	115	105	49	46
		100	74	112	73	33	33
			89	130	91	34	33
		200	84	126	66	26	24
			81	124	53	22	24
		400	77	110	77	34	35
			69	107	77	37	35
Positive Control	Methyl Methane Sulfonate	15	19	16	75	135	161*
			12	14	69	186	161*
	Methyl Methane Sulfonate	250	58	86	335	192	202*
			64	93	409	212	202*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	65	78	25	13	20
			69	84	38	18
			84	120	47	19
			83	119	72	29
Test Chemical	Calcium cyanamide	100	61	76	73	40	31
			81	99	52	21	31
		200	86	92	41	16	16
			69	80	33	16	16
		300	65	23	111	57	45*
			71	54	72	34	45*
		400

Positive Control	Methyl Methane Sulfonate	15	41	30	132	109	127*
Positive Control			34	26	148	145	127*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	67	95	40	20	19
			73	105	55	25
			66#	98	29	15
			73	102	34	16
Test Chemical	Calcium cyanamide	50	79	103	56	24	24
			85	100	60	24	24
		100	75#	82	63	28	25
			76	113	48	21	25
		200	62#	70	89	48	45*
			65	67	81	41	45*
		300	64	33	139	73	99*
			56	23	210	125	99*
		400

Positive Control	Methyl Methane Sulfonate	15	25	15	107	146	142*
Positive Control			21	13	86	139	142*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	76	107	81	36	35
			74	106	72	32
			68	115	65	32
			60	72	69	38
Test Chemical	Calcium cyanamide	100	62	88	75	40	35
			52	85	45	29	35
		250	40	29	93	78	62*
			51	51	72	47	62*
		400	66	13	472	238	238*
			60	15	427	238	238*
		550

Positive Control	3-Methylcholanthrene	2.5	67	58	509	253	288*
Positive Control			51	54	491	323	288*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	82	112	71	29	26
			65	103	32	16
			58	99	54	31
			52	86	43	27
Test Chemical	Calcium cyanamide	200	63	32	101	54	43
			71	35	70	33	43
		250	81	14	120	49	49*
			66	29	95	48	49*
		300	71	17	233	110	107*
			76	17	237	105	107*
		350	72	13	417	192	189*
			62	12	343	186	189*
		400	50	9	363	244	227*
			54	10	337	210	227*
		450

Positive Control	3-Methylcholanthrene	2.5	36	19	432	404	393*
Positive Control			43	23	495	382	393*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination