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Study Information
Study Attribute Value
CEBS Accession Number: 002-01821-0010-0000-5
Chemical Name: Calcium cyanamide
CASRN: 156-62-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 430294
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 47r 91 101 72 57
    57 102 104 61
    55 98 87 53
    42r 74 102 81
Test Chemical Calcium cyanamide 
25         
 85 147 84 33 36
    75 120 86 38
  
50         
 59 106 76 43 46
    71 115 105 49
  
100         
 74 112 73 33 33
    89 130 91 34
  
200         
 84 126 66 26 24
    81 124 53 22
  
400         
 77 110 77 34 35
    69 107 77 37
Positive Control Methyl Methane Sulfonate 
15         
 19 16 75 135 161*
    12 14 69 186
Methyl Methane Sulfonate 
250         
 58 86 335 192 202*
    64 93 409 212

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 65 78 25 13 20
    69 84 38 18
    84 120 47 19
    83 119 72 29
Test Chemical Calcium cyanamide 
100         
 61 76 73 40 31
    81 99 52 21
  
200         
 86 92 41 16 16
    69 80 33 16
  
300         
 65 23 111 57 45*
    71 54 72 34
  
400         
   
Positive Control Methyl Methane Sulfonate 
15         
 41 30 132 109 127*
    34 26 148 145

Nonactivation Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 67 95 40 20 19
    73 105 55 25
    66# 98 29 15
    73 102 34 16
Test Chemical Calcium cyanamide 
50         
 79 103 56 24 24
    85 100 60 24
  
100         
 75# 82 63 28 25
    76 113 48 21
  
200         
 62# 70 89 48 45*
    65 67 81 41
  
300         
 64 33 139 73 99*
    56 23 210 125
  
400         
   
Positive Control Methyl Methane Sulfonate 
15         
 25 15 107 146 142*
    21 13 86 139

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 76 107 81 36 35
    74 106 72 32
    68 115 65 32
    60 72 69 38
Test Chemical Calcium cyanamide 
100         
 62 88 75 40 35
    52 85 45 29
  
250         
 40 29 93 78 62*
    51 51 72 47
  
400         
 66 13 472 238 238*
    60 15 427 238
  
550         
   
Positive Control 3-Methylcholanthrene 
2.5       
 67 58 509 253 288*
    51 54 491 323

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 82 112 71 29 26
    65 103 32 16
    58 99 54 31
    52 86 43 27
Test Chemical Calcium cyanamide 
200         
 63 32 101 54 43
    71 35 70 33
  
250         
 81 14 120 49 49*
    66 29 95 48
  
300         
 71 17 233 110 107*
    76 17 237 105
  
350         
 72 13 417 192 189*
    62 12 343 186
  
400         
 50 9 363 244 227*
    54 10 337 210
  
450         
   
Positive Control 3-Methylcholanthrene 
2.5       
 36 19 432 404 393*
    43 23 495 382

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination