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Study Information
Study Attribute Value
CEBS Accession Number: 002-01824-0010-0000-8
Chemical Name: Caprolactam
CASRN: 105-60-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 127633
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 96 91 114 40 29
    80# 96 70 29
    86# 113 47 18
Test Chemical Caprolactam 
500         
 93# 110 102 37 31
    93 115 92 33
    106 121 79 25
  
1000         
 67# 89 70 35 35
    85# 88 92 36
    81 107 84 35
  
2000         
 108# 90 124 38 38
    77 87 104 45
    80# 85 77 32
  
3000         
 100# 82 99 33 39
    77 74 91 40
    89 82 118 44
  
4000         
 109 89 82 25 30
    110 115 105 32
    107# 98 104 32
  
5000         
 86 105 89 35 34
    102 88 112 37
    85 80 76 30
Positive Control Ethyl Methane Sulfonate 
250         
 76 75 817 361 327*
    82 74 718 292

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 88 95 81 31 29
    98 103 62 21
    77 102 79 34
    50r 44 61 41
Test Chemical Caprolactam 
500         
 84 96 73 29 30
    69# 116 82 40
    76# 81 50 22
  
1000         
 68 87 74 37 28
    102 99 42 14
    66 110 66 33
  
2000         
 82 120 75 31 29
    84 57 52 21
    59 69 62 35
  
3000         
 60# 60 71 39 30
    63 53 53 28
    65 70 43 22
  
4000         
 86 70 90 35 42
    67 64 101 50
    89 79 109 41
  
5000         
 70 61 82 39 39
    59 76 73 41
    77 63 88 38
Positive Control Methyl Methane Sulfonate 
5         
 56 40 570 340 353*
    56 37 611 366
Methyl Methane Sulfonate 
10         
 21 8 515 831 799*
    17 6 380 768

Non-Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 104 103 196 63 78
    91# 91 185 68
    98 79 327 112
    118 128 250 71
Test Chemical Caprolactam 
500         
 66 72 266 134 118*
    76 78 222 97
    74 70 269 121
  
1000         
 72 91 265 122 124*
    71 80 240 113
    64 86 260 136
  
2000         
 56 82 245 146 134*
    80 100 281 117
    76 78 316 140
  
3000         
 88 95 329 124 118*
    82 119 270 109
    69 95 249 120
  
4000         
 78 99 266 114 119*
    87 92 365 140
    79 93 249 105
  
5000         
 106# 101 305 96 99
    98 128 300 102
Positive Control 3-Methylcholanthrene 
3.5       
 53# 23 880 550 419*
    62 26 680 365
    79 32 810 342

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 101 87 98 32 32
    90 96 126 47
    114 104 93 27
    103 112 71 23
Test Chemical Caprolactam 
500         
 78 71 62 27 21
    124r 79 89 24
    102 103 49 16
  
1000         
 121r 93 104 29 27
    106 85 67 21
    85 80 85 33
  
2000         
 104 91 87 28 33
    99 86 96 32
    106 114 123 39
  
3000         
 90 95 87 32 43
    86 95 102 39
    95 112 163 57
  
4000         
 114 92 108 32 33
    107 87 99 31
    79# 85 85 36
  
5000         
 97 82 94 32 28
    90# 111 64 24
Positive Control 3-Methylcholanthrene 
3.5       
 86 59 365 141 144*
    118 58 487 137
    76 56 352 155

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 72 128 29 13 17
    69 89 35 17
    55 84 34 21
Test Chemical Caprolactam 
500         
 55# 84 31 19 20
    63 90 34 18
    53# 70 38 24
  
1000         
 62# 80 44 24 20
    69# 86 33 16
    63# 111 39 21
  
2000         
 68 54 63 31
  
3000         
 73 77 48 22 28
    52# 73 53 34
  
5000         
 53 76 20 13
Positive Control 3-Methylcholanthrene 
2.5       
 89 61 353 132 132*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109 89 151 46 44
    106 145 154 48
    84 87 139 55
    95 79 79 28
Test Chemical Caprolactam 
500         
 77 76 127 55 47
    80 71 122 51
    109# 69 118 36
  
1000         
 98 63 100 34 46
    74 64 106 48
    66 61 111 56
  
2000         
 84 63 96 38 47
    78 70 91 39
    79 57 151 64
  
3000         
 102 74 151 50 54
    85 57 155 61
    105 57 161 51
  
4000         
 103 91 163 53 55
    106 59 175 55
    84 62 147 58
  
5000         
 116 80 150 43 50
    106 102 199 62
    115 95 148 43
Positive Control 3-Methylcholanthrene 
2.5       
 57 17 682 398 319*
    80 49 679 285
    84 56 691 275

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination