Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-01836-0017-0000-8
Chemical Name: Carisoprodol
CASRN: 78-44-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 071860
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 112 105 89 26 34
    89 113 99 37
    100 105 102 34
    84 77 92 36
Test Chemical Carisoprodol 
250         
 90 70 73 27 30
    145r 114 91 21
    107 77 105 33
  
375         
 90 92 86 32 31
    84 91 74 29
    87 64 85 33
  
500         
 99 74 80 27 29
    80 48 83 34
    80 76 64 27
  
750         
 104 53 108 35 42
    80 42 117 49
    105 54 131 41
  
1000         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 94 69 506 179 198*
    77 70 496 216

Nonactivation Trial: 2 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 97 100 125 43 42
    74 96 94 43
    122r 110 153 42
    88 104 107 41
Test Chemical Carisoprodol 
300         
 62# 79 83 45 46
    76 64 120 53
    78 87 95 40
  
400         
 72 37 158 73 65*
    84 54 163 65
    64 67 108 57
  
500         
 67 50 151 75 98*
    70 12 211 100
    62 17 222 119
  
600         
   
  
700         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 42 33 646 519 608*
    28 25 589 697

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 90 95 84 31 31
    82 92 80 33
    136r 129 125 31
    117 113 101 29
Test Chemical Carisoprodol 
300         
 97 79 66 23 26
    85 83 87 34
    91 91 58 21
  
400         
 72 74 136 63 40
    68 75 59 29
    96 55 81 28
  
500         
 92 70 128 47 50*
    100 52 164 54
    93 44 140 50
  
600         
 105 39 152 48 63*
    75 34 113 50
    84 17 225 89
  
700         
 99 78 108 36 38
    82 67 101 41
    86 81 98 38
  
800         
   
  
1000         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 82 49 573 234 255*
    71 49 617 290
    71 69 511 240

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 111 81 23 28
    104 109 96 31
    132r 100 107 27
    107 80 98 31
Test Chemical Carisoprodol 
500         
 67 21 123 62 50*
    109 31 154 47
    77 17 96 42
  
550         
 105 21 142 45 42
    103 18 145 47
    86 32 91 35
  
600         
 80 15 156 65 59*
    70 15 143 68
    88# 27 116 44
  
650         
 75 11 158 70
   
    126r 23 227 60
  
700         
 62*
    86 13 164 63
    91 24 167 61
  
750         
    63 4 224 119
   
  
800         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 83 45 608 243 282*
    73 51 725 333
    92 39 751 271

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 102 93 168 55 62
    109 117 189 58
    72 75 204 95
    114 115 143 42
Test Chemical Carisoprodol 
200         
 106 82 159 50 47
    89 92 99 37
    89# 68 143 54
  
400         
 98 76 148 50 39
    83 84 76 30
    112# 96 123 37
  
500         
 100 77 107 36 34
    106 66 94 29
    111 86 127 38
  
600         
 96 54 150 52 45
    97 79 136 47
    119 109 131 37
  
700         
 105 31 102 32 49
    88 45 139 53
    83 14 154 62
  
800         
 86 7 184 72 60
    116 19 170 49
   
  
1000         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 70 21 628 301 345*
    56 21 637 383
    67 23 705 352

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 93# 96 129 46 49
    112# 115 150 45
    69 89 116 56
Test Chemical Carisoprodol 
300         
 68 80 101 49
  
400         
 57# 60 121 71 72
    67# 70 145 72
  
500         
 94# 83 175 62 53
    85# 71 114 45
  
600         
 82# 72 82 33 29
    105 47 75 24
  
700         
 78 32 96 41 51
    69 7 127 62
  
800         
   
  
1000         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 45# 16 576 427 437*
    51# 24 683 448

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination