Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01836-0017-0000-8
Chemical Name:	Carisoprodol
CASRN:	78-44-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	071860
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	112	105	89	26	34
			89	113	99	37
			100	105	102	34
			84	77	92	36
Test Chemical	Carisoprodol	250	90	70	73	27	30
			145r	114	91	21
			107	77	105	33
		375	90	92	86	32	31
			84	91	74	29
			87	64	85	33
		500	99	74	80	27	29
			80	48	83	34
			80	76	64	27
		750	104	53	108	35	42
			80	42	117	49
			105	54	131	41
		1000


Positive Control	Methyl Methane Sulfonate	5	94	69	506	179	198*
Positive Control			77	70	496	216	198*

Nonactivation Trial: 2 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	97	100	125	43	42
			74	96	94	43
			122r	110	153	42
			88	104	107	41
Test Chemical	Carisoprodol	300	62#	79	83	45	46
			76	64	120	53
			78	87	95	40
		400	72	37	158	73	65*
			84	54	163	65
			64	67	108	57
		500	67	50	151	75	98*
			70	12	211	100
			62	17	222	119
		600

		700


Positive Control	Methyl Methane Sulfonate	5	42	33	646	519	608*
Positive Control			28	25	589	697	608*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	90	95	84	31	31
			82	92	80	33
			136r	129	125	31
			117	113	101	29
Test Chemical	Carisoprodol	300	97	79	66	23	26
			85	83	87	34
			91	91	58	21
		400	72	74	136	63	40
			68	75	59	29
			96	55	81	28
		500	92	70	128	47	50*
			100	52	164	54
			93	44	140	50
		600	105	39	152	48	63*
			75	34	113	50
			84	17	225	89
		700	99	78	108	36	38
			82	67	101	41
			86	81	98	38
		800

		1000


Positive Control	Methyl Methane Sulfonate	5	82	49	573	234	255*
			71	49	617	290
			71	69	511	240

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	115	111	81	23	28
			104	109	96	31
			132r	100	107	27
			107	80	98	31
Test Chemical	Carisoprodol	500	67	21	123	62	50*
			109	31	154	47
			77	17	96	42
		550	105	21	142	45	42
			103	18	145	47
			86	32	91	35
		600	80	15	156	65	59*
			70	15	143	68
			88#	27	116	44
		650	75	11	158	70

			126r	23	227	60
		700					62*
			86	13	164	63
			91	24	167	61
		750
			63	4	224	119

		800


Positive Control	Methyl Methane Sulfonate	5	83	45	608	243	282*
			73	51	725	333
			92	39	751	271

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	102	93	168	55	62
			109	117	189	58
			72	75	204	95
			114	115	143	42
Test Chemical	Carisoprodol	200	106	82	159	50	47
			89	92	99	37
			89#	68	143	54
		400	98	76	148	50	39
			83	84	76	30
			112#	96	123	37
		500	100	77	107	36	34
			106	66	94	29
			111	86	127	38
		600	96	54	150	52	45
			97	79	136	47
			119	109	131	37
		700	105	31	102	32	49
			88	45	139	53
			83	14	154	62
		800	86	7	184	72	60
			116	19	170	49

		1000


Positive Control	3-Methylcholanthrene	2.5	70	21	628	301	345*
			56	21	637	383
			67	23	705	352

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	93#	96	129	46	49
			112#	115	150	45
			69	89	116	56
Test Chemical	Carisoprodol	300	68	80	101	49
		400	57#	60	121	71	72
			67#	70	145	72	72
		500	94#	83	175	62	53
			85#	71	114	45	53
		600	82#	72	82	33	29
			105	47	75	24	29
		700	78	32	96	41	51
			69	7	127	62	51
		800

		1000


Positive Control	3-Methylcholanthrene	2.5	45#	16	576	427	437*
Positive Control			51#	24	683	448	437*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination