Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01849-0001-0000-5 |
Chemical Name: | Chlorambucil |
CASRN: | 305-03-3 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 586147_SCE |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
No Activation | 2 | Positive |
Induced Rat Liver S9 | 1_S9 | Positive |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1049 | 412 | 0.393 | 8.24 | 26.5 | 0.000 |
Test Chemical | Chlorambucil | 0.2 | 5 | 104 | 383 | 3.683 | 76.60 | 26.5 | 837.656 |
0.5 | 5 | 105 | 613 | 5.838 | 122.60 | 26.5 | 1386.447 | ||
1.7 | 5 | 104 | 573 | 5.510 | 114.60 | 36.0 | 1302.812 | ||
5 | 0 | 0 | 0 | 0.000 | 0.00 | 36.0 | N/A | ||
Positive Control | Mitomycin-C | 0.001 | 50 | 1049 | 564 | 0.538 | 11.28 | 26.5 | 36.893 |
Mitomycin-C | 0.01 | 5 | 104 | 245 | 2.356 | 49.00 | 26.5 | 499.806 | |
Trend: | 58.065 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 25 | 525 | 219 | 0.417 | 8.76 | 25.5 | 0.000 |
Test Chemical | Chlorambucil | 3 | 5 | 105 | 419 | 3.990 | 83.80 | 35.0 | 856.621 |
4 | 5 | 104 | 596 | 5.731 | 119.20 | 35.0 | 1273.815 | ||
5 | 5 | 105 | 614 | 5.848 | 122.80 | 35.0 | 1301.826 | ||
Positive Control | Mitomycin-C | 0.001 | 25 | 525 | 326 | 0.621 | 13.04 | 25.5 | 48.858 |
Mitomycin-C | 0.01 | 5 | 105 | 236 | 2.248 | 47.20 | 25.5 | 438.813 | |
Trend: | 44.421 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1049 | 437 | 0.417 | 8.74 | 26.5 | 0.000 |
Test Chemical | Chlorambucil | 0.5 | 5 | 104 | 514 | 4.942 | 102.80 | 26.5 | 1086.380 |
1.7 | 5 | 104 | 549 | 5.279 | 109.80 | 36.0 | 1167.165 | ||
5 | 0 | 0 | 0 | 0.000 | 0.00 | 36.0 | N/A | ||
16.7 | 5 | 105 | 602 | 5.733 | 120.40 | 36.0 | 1276.262 | ||
50 | 0 | 0 | 0 | 0.000 | 0.00 | 0.0 | N/A | ||
Positive Control | Cyclophosphamide | 0.4 | 50 | 1049 | 547 | 0.521 | 10.94 | 26.5 | 25.172 |
Cyclophosphamide | 2 | 5 | 105 | 154 | 1.467 | 30.80 | 26.5 | 252.067 | |
Trend: | 54.780 | ||||||||
Probability: | 0.000 |