Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01849-0002-0000-6 |
Chemical Name: | Chlorambucil |
CASRN: | 305-03-3 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 586147_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
Induced Rat Liver S9 | 1_S9 | Positive |
No Activation | 2 | Positive |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 50 | 2 | 0.040 | 4.0 | 1 | 0.020 | 2.0 | 1 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 50 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | Chlorambucil | 50.2 | 10 | 56 | 5.600 | 100.0 | 4 | 0.400 | 20.0 | 11 | 1.100 | 60.0 | 41 | 4.100 | 50.0 |
100.5 | 10 | 10 | 1.000 | 100.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 10 | 1.000 | 100.0 | ||
150 | 10 | 10 | 1.000 | 100.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 10 | 1.000 | 100.0 | ||
Positive Control: | Mitomycin-C | 0.15 | 50 | 9 | 0.180 | 14.0 | 4 | 0.080 | 8.0 | 5 | 0.100 | 6.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.5 | 25 | 11 | 0.440 | 44.0 | 6 | 0.240 | 24.0 | 5 | 0.200 | 20.0 | 0 | 0.000 | 0.0 | |
Trend: | 7.999 | 0.274 | 0.487 | ||||||||||||
Probability: | 0.000 | 0.392 | 0.313 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | Chlorambucil | 12.6 | 10 | 36 | 3.600 | 100.0 | 17 | 1.700 | 90.0 | 9 | 0.900 | 50.0 | 10 | 1.000 | 10.0 |
25.2 | 10 | 64 | 6.400 | 100.0 | 8 | 0.800 | 40.0 | 16 | 1.600 | 50.0 | 40 | 4.000 | 40.0 | ||
50.3 | 10 | 100 | 10.000 | 100.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 100 | 10.000 | 100.0 | ||
100.5 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 6.25 | 100 | 16 | 0.160 | 15.0 | 5 | 0.050 | 5.0 | 11 | 0.110 | 11.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 12.5 | 25 | 18 | 0.720 | 48.0 | 1 | 0.040 | 4.0 | 17 | 0.680 | 44.0 | 0 | 0.000 | 0.0 | |
Trend: | 10.118 | 3.461 | 3.366 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | Chlorambucil | 20 | 25 | 30 | 1.200 | 56.0 | 8 | 0.320 | 24.0 | 12 | 0.480 | 40.0 | 10 | 0.400 | 4.0 |
25 | 25 | 36 | 1.440 | 56.0 | 11 | 0.440 | 40.0 | 5 | 0.200 | 20.0 | 20 | 0.800 | 8.0 | ||
50 | 10 | 58 | 5.800 | 90.0 | 4 | 0.400 | 30.0 | 4 | 0.400 | 30.0 | 50 | 5.000 | 5.0 | ||
Positive Control: | Mitomycin-C | 0.15 | 100 | 43 | 0.430 | 24.0 | 17 | 0.170 | 15.0 | 16 | 0.160 | 12.0 | 10 | 0.100 | 1.0 |
Mitomycin-C | 0.5 | 25 | 22 | 0.880 | 48.0 | 14 | 0.560 | 36.0 | 8 | 0.320 | 24.0 | 0 | 0.000 | 0.0 | |
Trend: | 8.083 | 5.404 | 3.691 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |