Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01852-0005-0000-3
Chemical Name:	Chloramphenicol sodium succinate
CASRN:	982-57-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	123527
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	65	97	138	71	68
			74	105	158	71
			70	104	125	60
			70	94	150	72
Test Chemical	Chloramphenicol sodium succinate	1310	79	80	175	74	103
			73	77	286	131	103
		1640	69	59	195	94	95
			71	63	206	96	95
		2050	82	65	197	80	72
			89	76	169	64	72
		2560	71	36	175	82	88
			66	41	183	93	88
		3200	66	33	242	123

		4000	67	16	220	110	126*
			57	13	240	142	126*
		5000

Positive Control	Ethyl Methane Sulfonate	500	38	34	1025	891	797*
			41	33	951	767
			47	40	1033	733

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	121	38	17	26
			73	85	53	24
			68#	108	57	28
			63	86	61	32
Test Chemical	Chloramphenicol sodium succinate	1310	50	46	53	35	35
			55	57	57	35	35
		1638	58	42	68	39	28
			48	50	25	17	28
		2048	68	45	52	25	23
			67	48	42	21	23
		2560	66	36	49	25	24
			66	35	44	22	24
		3200	64	20	54	28	31
			50	17	52	34	31
		4000	25	4	51	69	74*
			33	4	76	78	74*
		5000

Positive Control	Ethyl Methane Sulfonate	500	41	32	677	550	523*
			38	46	672	587
			46	44	594	432

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	65#	86	112.5	58	57
			66	94	103	52
			74	114	122	55
			78	105	150	64
Test Chemical	Chloramphenicol sodium succinate	1048	80	93	151	63
		1310	70	67	156	75	68
			72	77	132	62	68
		1638	65#	54	211.5	108	92*
			61#	51	138	75	92*
		2048	75	50	212	94	85
			82#	54	187.5	76	85
		2560	77	37	237	102	107*
			75#	34	252	112	107*
		3200	75	25	264	117	110*
			83	28	254	102	110*
		4000	61	14	316	172	182*
			61	14	348	192	182*
Positive Control	3-Methylcholanthrene	5	57	60	465	272	254*
			62	61	411	222
			53	53	428	269

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	69	106	74	36	36
			71	98	83	39
			65	94	70	36
			70	102	73	35
Test Chemical	Chloramphenicol sodium succinate	1048	68	65	152	75	70*
			58#	58	126	72
			72#	65	137	64
		1310	54	47	114	71	86*
			52	48	145	93
			57	49	160	93
		1638	45	33	133	98	92*
			52	37	138	88
			52	38	140	89
		2048	43	27	164	129	113*
			44	24	130	98
			44	28	150	114
		2560	57	24	190	111	126*
			47	21	168	120
			52	24	229	147
		3200	51	16	271	179	147*
			50	15	184	122
			56	18	236	140
		4000	31	5	290	312	318*
			31	5	302	330
			31	6	294	313
		5000


Positive Control	3-Methylcholanthrene	5	41	36	457	376	330*
			40	37	418	348
			48	43	379	266

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination