Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01852-0006-0000-4
Chemical Name:	Chloramphenicol sodium succinate
CASRN:	982-57-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	417989
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	101	117	74	25	24
			104	100	82	26
			97	82	70	24
			100	101	60	20
Test Chemical	Chloramphenicol sodium succinate	250	101	85	48	16	19
			98	86	65	22
			102	93	59	19
		500	107	110	45	14	18
			101	112	49	16
			93#	78	69	25
		1000	103	89	53	17	17
			97#	98	48	16
			100	102	52	17
		2000	96	70	62	21	22
			111	88	76	23	22
		3000	92	31	100	36	28
			107	41	61	19	28
		4000	114#	52	58	17	20
			100	49	71	24	20
		5000

Positive Control	Ethyl Methane Sulfonate	500	28	11	780	929	722*
Positive Control			55	24	849	516	722*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	82	102	75	30	20
			81#	92	43.5	18
			82	106	29	12
			125r	136	34	9
Test Chemical	Chloramphenicol sodium succinate	500	102	113	46	15	15
			138r	133	40	10
			83	91	39	16
		1000	196r	161	45	8
			136r	99	44	11
			110	90	46	14
		2000	82	60	58	24	24
			99	49	72	24
			78	56	53	23
		3000	101	26	61	20	22
			75	29	59	26
			100	34	58	19
		4000	65	9	101	52	47*
			73	9	96	44
			72	13	98	45
		4500


Positive Control	Ethyl Methane Sulfonate	500	13	5	282	723	725*
			14	5	356	828
			14	4	265	624

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	89	91	77	29	22
			102	125	54	18
			82	100	48	20
			80	84	54	23
Test Chemical	Chloramphenicol sodium succinate	500	97#	85	33	11	15
			90	61	48	18
			84	44	39	15
		1000	92	50	67	24	22
			70	52	41	19
			92	56	63	23
		2000	95	27	64	22	23
			110	36	72	22
			89	33	65	24
		3000	74	8	114	51	54*
			61	6	102	56	54*
		3500					71*
			66	4	117	59
			58	5	143	82
		4000


Positive Control	Ethyl Methane Sulfonate	500	10	3	415	1339	1329*
			16#	6	552	1187
			13	4	562	1460

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	83	93	122	49	63
			77	102	141	61
			76	98	170	75
			79	107	163	69
Test Chemical	Chloramphenicol sodium succinate	1000	80	93	122	51	55
			58	75	101	58
			86	81	145	57
		2000	80	77	146	61	81
			59	48	172	97
			79	59	203	86
		3000	71	31	250	117	91
			66	38	117	60
			73	49	214	97
		4000	55	16	256	154	164*
			54	15	262	162
			42	11	222	175
		4500	50	13	177	118	116*
			48	14	195	137
			78	20	216	93
		5000	41	9	223	181	182*
			35	8	197	188
			40	7	210	177
Positive Control	3-Methylcholanthrene	7	47	18	250	179	211*
			28	12	226	267
			46	19	256	188

Non-Induced S9 Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	78	99	206	89	78
			79	76	210	89
			93	126	166	59
			84	99	185	73
Test Chemical	Chloramphenicol sodium succinate	1000	94	74	219	77	82
			87	57	191	73
			71	74	204	96
		2000	64	27	191	99	108
			41	14	154	124
			51	14	154	101
		3000					245*
			26	2	136	173
			22	2	212	316
		4000


Positive Control	3-Methylcholanthrene	7	44	9	268	202	196*
			43	9	219	168
			37	9	240	217

Non-Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	66	98	186	94	82
			68	82	180	89
			70	115	139	66
			97	105	224	77
Test Chemical	Chloramphenicol sodium succinate	500	52	95	165	106	92
			45	81	154	115
			58	110	96	56
		1000	56	94	188	112	92
			66#	113	157.5	80
			62	89	157	84
		2000	50	60	180	120	147*
			50#	42	221	147
			36	32	186	173
		2500	53	22	285	179	200*
			33	16	231	232
			30#	29	169.5	189
		3000	29	10	151	177	193*
			44	11	280	212
			34	7	193	190
		3500


Positive Control	3-Methylcholanthrene	7	36	26	261	242	249*
			43#	13	269	207
			43	11	384	297

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	71	83	55	26	25
			74	81	62	28
			67	140	48	24
			74	95	50	23
Test Chemical	Chloramphenicol sodium succinate	500	60#	79	42	23	27
			76#	45	73.5	32
			72	65	55	25
		1000	73	82	73	34	24
			89	58	57	21
			71	88	38	18
		2000	62	64	46	25	24
			71	84	64	30
			78	62	40	17
		3000	54#	32	66	40	32
			70	41	50	24
			80	46	76	32
		4000	74	45	66	30	30
			58	46	67	38
			72	54	45	21
		5000	60	27	83	46	45*
			56	11	79	47
			60	22	73	40
Positive Control	3-Methylcholanthrene	5	22#	2	224	343	317*
			26	8	256	328
			21	5	178	280

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86	104	168	65	73
			69	95	191	92
			93	91	206	74
			94#	110	167	59
Test Chemical	Chloramphenicol sodium succinate	1000	65	61	136	69	72
			76	86	151	66
			73#	84	174	79
		2000	73	41	282	128	100
			72	68	174	81
			68	62	187	92
		3000	38	19	166	145	141*
			49	13	198	135
			67	22	285	141
		4000	42	11	256	206	306*
			45	8	366	273
			28	2	364	439
		4500					329*
			26	3	246	317
			24	2	240	340
		5000


Positive Control	3-Methylcholanthrene	5	13#	1	135	337	339*
			12	1	121	341
			5r	0	127	794

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination