Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01854-0009-0000-9
Chemical Name:	Chlordane (technical grade)
CASRN:	12789-03-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	272427
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	104	117	48	54
			88	99	168	64
			62	99	110	59
			77	99	104	45
Test Chemical	Chlordane (technical grade)	5	83	102	151	61	64
			82	90	163	67	64
		15	67	74	189	94	80
			76	79	151	66	80
		25	72	34	418	194	167*
			96#	42	401	140	167*
		35	56	6	466	277	236*
			74	6	432	196	236*
		45

Positive Control	Methyl Methane Sulfonate	15	36	26	410	380	428*
Positive Control			27	27	386	477	428*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113	94	154	45	46
			107	98	139	44
			99	109	152	51
			104	98	132	43
Test Chemical	Chlordane (technical grade)	25	99	70	156	53	56
			85	67	150	59	56
		30	85	51	244	96	79*
			90	55	170	63	79*
		35	77	33	468	203	156*
			55	42	182	110	156*
		40	81	26	420	173	263*
			77	21	815	354	263*
		45	85	16	1060	417	576*
			39#	11	860	735	576*
Positive Control	Methyl Methane Sulfonate	15	59	39	471	266	239*
Positive Control			55	44	346	211	239*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination