Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01855-0012-0000-4
Chemical Name:	Chlordecone (kepone)
CASRN:	143-50-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	163075
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	100	74	37	32
			97	95	82	28
			94	90	77	27
			111	115	119	36
Test Chemical	Chlordecone (kepone)	1	91	100	82	30	37
			104	99	136	43	37
		2	79	92	68	29	32
			106	109	113	36	32
		4	84	90	75	30	29
			80	98	66	27	29
		8	102	85	90	30	28
			103	97	83	27	28
		16	88	83	65	25	30
			75	75	81	36	30
Positive Control	Methyl Methane Sulfonate	15	42	23	455	361	367*
Positive Control			36	23	401	373	367*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	102	70	35	28
			75	105	94	42
			78	109	31	13
			78	84	54	23
Test Chemical	Chlordecone (kepone)	15	61	101	80	44	31
			43	51	22	17	31
		20	35	46	48	46	46
			72	100	99	46	46
		25	55	63	40	24	40
			69	71	116	56	40
		30	78	149	122	52	58*
			57	72	110	65	58*
		35	84	50	123	49	46*
			89	35	114	43	46*
Positive Control	Methyl Methane Sulfonate	15	44	38	170	130	140*
Positive Control			44	27	197	150	140*

Nonactivation Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	127	109	36	36
			84	99	104	42
			94	76	100	36
			99	98	94	32
Test Chemical	Chlordecone (kepone)	5	88	96	136	51	60*
			95	83	195	68	60*
		10	105	107	120	38	39
			105	112	125	40	39
		15	83	62	97	39	49
			87	67	153	59	49
		25

Positive Control	Methyl Methane Sulfonate	15	36	20	252	237	240*
Positive Control			51	23	372	244	240*

Nonactivation Trial: 4 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	97	276	93	74
			106	89	194	61
			97	84	211	73
			114	130	234	69
Test Chemical	Chlordecone (kepone)	5	92	94	217	79	72
			113	124	217	64	72
		10	90	92	239	89	85
			91	81	221	81	85
		15	87	81	193	74	86
			90	86	264	97	86
		20

Positive Control	Methyl Methane Sulfonate	15	37	23	146	133	144*
Positive Control			39	25	179	154	144*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	103	38	14	17
			78	101	22	9
			87	115	43	16
			65	82	56	29
Test Chemical	Chlordecone (kepone)	10	80	89	42	18	15
			88	117	34	13	15
		15	91	78	63	23	20
			72	67	38	18	20
		20	40	20	61	51	40*
			57	29	52	30	40*
		25

Positive Control	Methyl Methane Sulfonate	15	39	29	162	140	129*
Positive Control			44	42	156	118	129*

Nonactivation Trial: 6 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	92	36	19	21
			74	96	58	26
			74	91	68	31
			64	121	13	7
Test Chemical	Chlordecone (kepone)	15	88	79	43	16	13
			62	64	18	10	13
		18	77#	94	76.5	33	38*
			48#	51	63	43	38*
		21	47	38	16	11	18
			50	36	37	25	18
		24	28	7	139	165	133*
			28	8	84	101	133*
		27	11	1	1006	3048	2754*
			14	1	1058	2460	2754*
Positive Control	Methyl Methane Sulfonate	15	34	23	151	147	136*
Positive Control			35	17	130	126	136*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	114	53	20	17
			114	108	48	14
			87	88	35	13
			72	90	47	22
Test Chemical	Chlordecone (kepone)	15	84	82	39	15	16
			83	75	42	17	16
		20	93	83	39	14	15
			83	78	42	17	15
		25	89	83	56	21
			125r	86	64	17
		30	103	59	39	13	10
			92	66	22	8	10
		35	88	25	178	68	79*
			117	36	317	91	79*
		40

Positive Control	3-Methylcholanthrene	2.5	74	34	293	133	134*
Positive Control			71	34	287	134	134*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	102	38	15	14
			99	106	32	11
			70	95	49	23
			82	98	22	9
Test Chemical	Chlordecone (kepone)	15	67	120	36	18	18
			89	119	49	18	18
		20	88	102	48	18	17
			88	100	40	15	17
		25	74	79	62	28	24
			67	91	38	19	24
		30	59	69	52	29	24
			65	61	34	18	24
		35	57	34	75	44	32*
			63	41	39	21	32*
		40

Positive Control	3-Methylcholanthrene	2.5	47	41	194	138	140*
Positive Control			45#	45	189	142	140*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	53	95	53	33	29
			60	75	49	27
			60	109	36	20
			51	121	52	34
Test Chemical	Chlordecone (kepone)	10	65#	101	89	45	35
			67	111	50	25	35
		15	78	105	71	30	27
			96	92	65	23	27
		20	58	58	46	26	25
			75	53	53	24	25
		25	61	48	49	27	35
			74	33	96	43	35
		30
		35

Positive Control	3-Methylcholanthrene	2.5	39	20	422	365	341*
Positive Control			41#	18	390	317	341*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination