Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01856-0009-0000-1
Chemical Name:	Chlorendic acid
CASRN:	115-28-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	197818
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	111	99	43	40
			64	75	68	36
			77	93	74	32
			73	121	112	51
Test Chemical	Chlorendic acid	62.5	73	102	86	39	40
			90	129	109	41	40
		125	71	123	95	45	40
			82	127	87	35	40
		250	88	83	68	26	33
			86	121	103	40	33
		500	81	133	94	39	38
			86	117	94	36	38
		1000	82	93	103	42	33
			81	79	57	23	33
		2000

Positive Control	Methyl Methane Sulfonate	15	32	19	278	294	291*
Positive Control			31	33	264	287	291*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	49r	59	113	77	39
			103	120	69	22
			42r	72	50	39
			58	80	96	55
Test Chemical	Chlorendic acid	700	42	57	67	53	60
			34	39	67	66	60
		900	48	40	90	62	59
			62#	56	103	55	59
		1100	59	35	91	51	53
			46	33	75	54	53
		1300	57	23	108	64	66*
			65	56	131	68	66*
		1500	47	22	149	105	125*
			49	18	210	144	125*
		1700

Positive Control	Methyl Methane Sulfonate	15	17	14	250	495	522*
Positive Control			11	10	181	548	522*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	96	142	52	50
			79	102	116	49
			94	94	127	45
			67	108	105	52
Test Chemical	Chlorendic acid	700	73	80	156	71	74
			68	81	156	77	74
		900	152r	78	224	49
			94	60	140	50
		1100	81	69	111	46	46
			79	67	107	45	46
		1300	55	60	91	55	49
			85	60	109	43	49
		1500	100	59	157	52	55
			97	34	167	57	55
		1700

Positive Control	Methyl Methane Sulfonate	15	32	21	410	434	416*
Positive Control			33	20	392	398	416*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	88	75	24	35
			114	107	112	33
			98	99	134	46
			109	106	118	36
Test Chemical	Chlorendic acid	1300	94	66	96	34	36
			98	71	109	37	36
		1400	104	49	139	44	38
			119	76	113	32	38
		1500	117	69	131	37	40
			93	69	121	43	40
		1600	113	73	113	33	38
			91	75	116	43	38
		1700	55	4	520	315	287*
			77	6	598	258	287*
Positive Control	Methyl Methane Sulfonate	15	69	34	520	253	300*
Positive Control			60	33	628	347	300*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination