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Study Information
Study Attribute Value
CEBS Accession Number: 002-01856-0009-0000-1
Chemical Name: Chlorendic acid
CASRN: 115-28-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 197818
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 111 99 43 40
    64 75 68 36
    77 93 74 32
    73 121 112 51
Test Chemical Chlorendic acid 
62.5       
 73 102 86 39 40
    90 129 109 41
  
125         
 71 123 95 45 40
    82 127 87 35
  
250         
 88 83 68 26 33
    86 121 103 40
  
500         
 81 133 94 39 38
    86 117 94 36
  
1000         
 82 93 103 42 33
    81 79 57 23
  
2000         
   
Positive Control Methyl Methane Sulfonate 
15         
 32 19 278 294 291*
    31 33 264 287

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 49r 59 113 77 39
    103 120 69 22
    42r 72 50 39
    58 80 96 55
Test Chemical Chlorendic acid 
700         
 42 57 67 53 60
    34 39 67 66
  
900         
 48 40 90 62 59
    62# 56 103 55
  
1100         
 59 35 91 51 53
    46 33 75 54
  
1300         
 57 23 108 64 66*
    65 56 131 68
  
1500         
 47 22 149 105 125*
    49 18 210 144
  
1700         
   
Positive Control Methyl Methane Sulfonate 
15         
 17 14 250 495 522*
    11 10 181 548

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 96 142 52 50
    79 102 116 49
    94 94 127 45
    67 108 105 52
Test Chemical Chlorendic acid 
700         
 73 80 156 71 74
    68 81 156 77
  
900         
 152r 78 224 49
    94 60 140 50
  
1100         
 81 69 111 46 46
    79 67 107 45
  
1300         
 55 60 91 55 49
    85 60 109 43
  
1500         
 100 59 157 52 55
    97 34 167 57
  
1700         
   
Positive Control Methyl Methane Sulfonate 
15         
 32 21 410 434 416*
    33 20 392 398

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 88 75 24 35
    114 107 112 33
    98 99 134 46
    109 106 118 36
Test Chemical Chlorendic acid 
1300         
 94 66 96 34 36
    98 71 109 37
  
1400         
 104 49 139 44 38
    119 76 113 32
  
1500         
 117 69 131 37 40
    93 69 121 43
  
1600         
 113 73 113 33 38
    91 75 116 43
  
1700         
 55 4 520 315 287*
    77 6 598 258
Positive Control Methyl Methane Sulfonate 
15         
 69 34 520 253 300*
    60 33 628 347

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination