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Study Information
Study Attribute Value
CEBS Accession Number: 002-01858-0014-0000-9
Chemical Name: Chlorinated paraffins: C23, 43% chlorine
CASRN: 108171-27-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 010900
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 74 78 75 34 22
    99 119 44 15
    82 109 58 24
    85 95 43 17
Test Chemical Chlorinated paraffins: C23, 43% chlorine 
3.13      
 98 117 45 15 19
    78 109 54 23
  
6.25      
 70 80 48 23 26
    90 118 80 30
  
12.5       
 74 105 51 23 19
    91 114 42 15
  
25         
 76 127 31 14 13
    93 91 35 13
  
50         
 91 106 84 31 25
    101 115 58 19
Positive Control Methyl Methane Sulfonate 
15         
 37 37 183 167 145*
    50 45 184 123

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 103 85 37 29
    75 95 51 23
    66 111 78 39
    70 91 34 16
Test Chemical Chlorinated paraffins: C23, 43% chlorine 
20         
 91 92 86 32 33
    70 104 70 34
  
30         
 79 96 66 28 26
    88 98 62 23
  
40         
 87 92 93 36 28
    78 107 48 21
  
50         
 70 90 52 25 27
    64 83 57 30
  
60         
 83 103 55 22 29
    62 99 68 36
Positive Control Methyl Methane Sulfonate 
15         
 35 34 174 167 133*
    35 30 105 99

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 52 80 117 76 47
    53 105 52 32
    67 97 91 45
    65 118 68 35
Test Chemical Chlorinated paraffins: C23, 43% chlorine 
20         
 58 108 68 39 36
    60 89 59 33
  
30         
 115 212 61 18 35
    61# 85 94.5 52
  
40         
 57 69 75 44 42
    58# 104 69 39
  
50         
 69 104 66 32 43
    73 116 118 54
  
60         
 76 117 85 37 36
    55 109 58 35
Positive Control 3-Methylcholanthrene 
2.5       
 22 16 250 376 433*
    22 13 328 490

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 84 62 27 28
    87 102 74 28
    70 76 66 32
    73 138 59 27
Test Chemical Chlorinated paraffins: C23, 43% chlorine 
20         
 70 76 52 25 23
    64 81 42 22
  
40         
 75 82 53 24 23
    67 71 45 22
  
60         
 69 107 52 25 23
    91 63 59 22
  
80         
 74 85 43 19 20
    63 100 40 21
  
100         
 67 75 44 22 24
    82 72 63 26
Positive Control 3-Methylcholanthrene 
2.5       
 34 16 263 258 257*
    43 19 328 256

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination