Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01858-0014-0000-9
Chemical Name:	Chlorinated paraffins: C23, 43% chlorine
CASRN:	108171-27-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	010900
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	74	78	75	34	22
			99	119	44	15
			82	109	58	24
			85	95	43	17
Test Chemical	Chlorinated paraffins: C23, 43% chlorine	3.13	98	117	45	15	19
			78	109	54	23	19
		6.25	70	80	48	23	26
			90	118	80	30	26
		12.5	74	105	51	23	19
			91	114	42	15	19
		25	76	127	31	14	13
			93	91	35	13	13
		50	91	106	84	31	25
			101	115	58	19	25
Positive Control	Methyl Methane Sulfonate	15	37	37	183	167	145*
Positive Control			50	45	184	123	145*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	103	85	37	29
			75	95	51	23
			66	111	78	39
			70	91	34	16
Test Chemical	Chlorinated paraffins: C23, 43% chlorine	20	91	92	86	32	33
			70	104	70	34	33
		30	79	96	66	28	26
			88	98	62	23	26
		40	87	92	93	36	28
			78	107	48	21	28
		50	70	90	52	25	27
			64	83	57	30	27
		60	83	103	55	22	29
			62	99	68	36	29
Positive Control	Methyl Methane Sulfonate	15	35	34	174	167	133*
Positive Control			35	30	105	99	133*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	52	80	117	76	47
			53	105	52	32
			67	97	91	45
			65	118	68	35
Test Chemical	Chlorinated paraffins: C23, 43% chlorine	20	58	108	68	39	36
			60	89	59	33	36
		30	115	212	61	18	35
			61#	85	94.5	52	35
		40	57	69	75	44	42
			58#	104	69	39	42
		50	69	104	66	32	43
			73	116	118	54	43
		60	76	117	85	37	36
			55	109	58	35	36
Positive Control	3-Methylcholanthrene	2.5	22	16	250	376	433*
Positive Control			22	13	328	490	433*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	84	62	27	28
			87	102	74	28
			70	76	66	32
			73	138	59	27
Test Chemical	Chlorinated paraffins: C23, 43% chlorine	20	70	76	52	25	23
			64	81	42	22	23
		40	75	82	53	24	23
			67	71	45	22	23
		60	69	107	52	25	23
			91	63	59	22	23
		80	74	85	43	19	20
			63	100	40	21	20
		100	67	75	44	22	24
			82	72	63	26	24
Positive Control	3-Methylcholanthrene	2.5	34	16	263	258	257*
Positive Control			43	19	328	256	257*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination