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Study Information
Study Attribute Value
CEBS Accession Number: 002-01858-0014-0000-9
Chemical Name: Chlorinated paraffins: C23, 43% chlorine
CASRN: 108171-27-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 010900
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone
 0         
74 78 75 34 22
    99 119 44 15
    82 109 58 24
    85 95 43 17
Test Chemical Chlorinated paraffins: C23, 43% chlorine
3.13      
98 117 45 15 19
    78 109 54 23
 
6.25      
70 80 48 23 26
    90 118 80 30
 
12.5       
74 105 51 23 19
    91 114 42 15
 
25         
76 127 31 14 13
    93 91 35 13
 
50         
91 106 84 31 25
    101 115 58 19
Positive Control Methyl Methane Sulfonate
15         
37 37 183 167 145*
    50 45 184 123

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
78 103 85 37 29
    75 95 51 23
    66 111 78 39
    70 91 34 16
Test Chemical Chlorinated paraffins: C23, 43% chlorine
20         
91 92 86 32 33
    70 104 70 34
 
30         
79 96 66 28 26
    88 98 62 23
 
40         
87 92 93 36 28
    78 107 48 21
 
50         
70 90 52 25 27
    64 83 57 30
 
60         
83 103 55 22 29
    62 99 68 36
Positive Control Methyl Methane Sulfonate
15         
35 34 174 167 133*
    35 30 105 99

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
52 80 117 76 47
    53 105 52 32
    67 97 91 45
    65 118 68 35
Test Chemical Chlorinated paraffins: C23, 43% chlorine
20         
58 108 68 39 36
    60 89 59 33
 
30         
115 212 61 18 35
    61# 85 94.5 52
 
40         
57 69 75 44 42
    58# 104 69 39
 
50         
69 104 66 32 43
    73 116 118 54
 
60         
76 117 85 37 36
    55 109 58 35
Positive Control 3-Methylcholanthrene
2.5       
22 16 250 376 433*
    22 13 328 490

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
77 84 62 27 28
    87 102 74 28
    70 76 66 32
    73 138 59 27
Test Chemical Chlorinated paraffins: C23, 43% chlorine
20         
70 76 52 25 23
    64 81 42 22
 
40         
75 82 53 24 23
    67 71 45 22
 
60         
69 107 52 25 23
    91 63 59 22
 
80         
74 85 43 19 20
    63 100 40 21
 
100         
67 75 44 22 24
    82 72 63 26
Positive Control 3-Methylcholanthrene
2.5       
34 16 263 258 257*
    43 19 328 256

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination