COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01864-0011-0000-3
Chemical Name: Chlorobenzilate
CASRN: 510-15-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 159650
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 121r 106 49 13 17
    105# 120 60 19
    102 93 61 20
    102 87 40 13
Test Chemical Chlorobenzilate 
6.25      
 106 76 32 10 11
    104 66 28 9
    103 60 42 14
  
12.5       
 100 57 40 13 14
    86 47 47 18
    78 63 24 10
  
25         
 76 30 47 21 20
    85 31 52 20
    95 36 51 18
  
50         
 81 19 43 18 16
    78 16 30 13
    98 27 48 16
  
75         
 60 10 26 14 29
    37 2 56 51
    67 6 41 20
  
100         
   
    86 8 43 17
  
200         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 85 47 850 333 365*
    81 44 925 380
    83 48 947 382

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 103 168 69 68
    105# 94 223 71
    121r 102 195 54
    115 103 225 65
Test Chemical Chlorobenzilate 
20         
 76# 37 171 75 72
    98# 44 194 66
    82# 35 187 76
  
30         
 91 32 193 71 60
    68# 30 126 62
    73# 30 103 47
  
40         
 92# 27 184 67 76
    82 23 201 81
    74 24 176 79
  
50         
 77# 18 199 86 80
    100 23 226 75
    66# 23 160 81
  
75         
 72# 13 251 117 94
    79# 17 139 59
    75# 3 239 107
  
125         
 68 6 256 126 113*
    75 4 260 116
    63 7 185 98
Positive Control Ethyl Methane Sulfonate 
250         
 70 43 1077 512 498*
    61# 46 942 513
    67 45 937 470

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87# 97 69 27 30
    89# 103 89 33
Test Chemical Chlorobenzilate 
10         
 65# 88 31 16 21
    70 104 51 24
    71# 83 49 23
  
20         
 58 95 43 25 25
    68 91 54 26
  
30         
 66# 82 26 13 19
    88# 110 50 19
    68# 77 53 26
  
50         
 82# 56 52 21 24
    83 71 65 26
    83# 97 59 24
  
70         
 82r 62 75 31
Positive Control 3-Methylcholanthrene 
3.5       
 93# 101 295 106 106*

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 38 71 26 27
    79 152 66 28
    79 110 61 26
Test Chemical Chlorobenzilate 
3.13      
 77 85 67 29 30
    49 72 43 29
    57 75 55 32
  
6.25      
 60 43 41 23 37
    58 58 80 46
    73 52 97 44
  
12.5       
 70 27 64 31 29
    69 19 39 19
    56 8 63 37
  
25         
   
    63 10 58 31
  
50         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 72# 60 394 182 177*
    69 27 356 173

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 107 94 28 28
    68 83 60 29
    87 114 66 25
    75 96 67 30
Test Chemical Chlorobenzilate 
20         
 116 82 99 28 26
    80 70 68 28
    92 73 56 20
  
30         
 81 59 122 50 39
    70 77 85 41
    101 57 79 26
  
40         
 122r 64 85 23 33
    103 66 73 24
    102 43 130 43
  
50         
 93 79 68 24 32
    76 52 85 37
    94 65 96 34
  
60         
 114 41 107 31 29
    102 81 80 26
    147r 64 98 22
  
80         
 74 34 78 35 28
    91 34 98 36
    96 49 42 15
Positive Control 3-Methylcholanthrene 
2.5       
 107 72 370 115 133*
    94 75 412 146
    107# 86 439 137

Induced S9 Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 79 131 44 42
    86 89 119 46
    76 107 85 38
    97 125 116 40
Test Chemical Chlorobenzilate 
20         
 119 69 183 51 58
    89 39 158 59
    82# 39 154 62
  
30         
 108 33 153 47 51
    71 27 125 59
    94 35 131 46
  
40         
 105 38 191 60 57
    98 27 166 56
    94 28 153 55
  
50         
 84 30 120 48 56
    98 18 163 55
    119 29 228 64
  
60         
 130r 16 155 40
    126r 40 99 26
    112 20 147 44
  
80         
 114 21 134 39 43
    69 8 99 48
    102 9 132 43
Positive Control 3-Methylcholanthrene 
2.5       
 85 44 715 279 290*
    67 49 605 300
    88# 49 774 292

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination