Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01867-0002-0000-6 |
Chemical Name: | Chloroform |
CASRN: | 67-66-3 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 564651_CA |
Study Result: | Equivocal |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Negative |
Induced Rat Liver S9 | 1_S9 | Weakly Positive |
Induced Rat Liver S9 | 2_S9 | Questionable |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Test Chemical: | Chloroform | 500 | 200 | 1 | 0.005 | 0.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
1000 | 200 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
1600 | 200 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 200 | 36 | 0.180 | 17.0 | 13 | 0.065 | 6.5 | 22 | 0.110 | 10.5 | 1 | 0.005 | 1.0 |
Mitomycin-C | 0.25 | 50 | 15 | 0.300 | 18.0 | 8 | 0.160 | 10.0 | 7 | 0.140 | 10.0 | 0 | 0.000 | 0.0 | |
Trend: | 0.355 | -0.480 | 0.687 | ||||||||||||
Probability: | 0.361 | 0.684 | 0.246 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | Chloroform | 1600 | 200 | 1 | 0.005 | 0.5 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
3000 | 200 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | ||
5000 | 200 | 7 | 0.035 | 3.5 | 1 | 0.005 | 0.5 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 2.5 | 200 | 34 | 0.170 | 16.0 | 12 | 0.060 | 5.5 | 21 | 0.105 | 10.5 | 1 | 0.005 | 1.0 |
Cyclophosphamide | 7.5 | 50 | 21 | 0.420 | 22.0 | 2 | 0.040 | 2.0 | 9 | 0.180 | 18.0 | 10 | 0.200 | 1.0 | |
Trend: | 3.075 | 0.594 | 3.164 | ||||||||||||
Probability: | 0.001 | 0.276 | 0.001 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 200 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 | 1 | 0.005 | 0.5 | 0 | 0.000 | 0.0 |
Test Chemical: | Chloroform | 1600 | 200 | 7 | 0.035 | 3.5 | 2 | 0.010 | 1.0 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 |
3000 | 200 | 4 | 0.020 | 2.0 | 1 | 0.005 | 0.5 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | ||
5000 | 200 | 3 | 0.015 | 1.0 | 0 | 0.000 | 0.0 | 3 | 0.015 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 2.5 | 200 | 28 | 0.140 | 12.0 | 3 | 0.015 | 1.5 | 25 | 0.125 | 11.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 7.5 | 50 | 17 | 0.340 | 34.0 | 3 | 0.060 | 6.0 | 14 | 0.280 | 28.0 | 0 | 0.000 | 0.0 | |
Trend: | 0.012 | -0.243 | 0.141 | ||||||||||||
Probability: | 0.495 | 0.596 | 0.444 |