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Study Information
Study Attribute Value
CEBS Accession Number: 002-01877-0010-0000-6
Chemical Name: Chlorpheniramine maleate
CASRN: 113-92-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 501757
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 97 129 46 45
    81 100 132 54
    94 104 100 36
    99 99 129 44
Test Chemical Chlorpheniramine maleate 
10         
 72 79 101 47 40
    96 88 96 33
  
50         
 61 51 130 72 59
    66 33 94 47
  
90         
 79r 13 152 64
    72 37 122 56
  
130         
 85 20 169 66 62
    95 18 166 58
  
170         
 75 8 145 64 63
    58 9 109 62
  
210         
   
Positive Control Methyl Methane Sulfonate 
15         
 43 11 462 360 360*
    35 30 383 361

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 87 125 42 36
    95 119 89 31
    86 102 77 30
    89 92 105 39
Test Chemical Chlorpheniramine maleate 
75         
 61 24 93 51 55
    68 31 120 59
  
150         
 99 22 110 37 41
    76 29 103 45
  
175         
 65 9 86 44 42
    84 22 103 41
  
200         
 84 14 61 24 27
    47 6 43 31
  
225         
 84 10 61 24 27
    45 9 40 30
  
250         
   
  
275         
   
Positive Control Methyl Methane Sulfonate 
15         
 77 74 87 38 34
    83 79 75 30
Methyl Methane Sulfonate 
250         
 67 80 398 199 186*
    67 85 350 173

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 104 108 50 55
    62 94 109 59
    64 106 118 62
    77 97 118 51
Test Chemical Chlorpheniramine maleate 
90         
 99 105 158 53 48
    101 123 131 43
  
120         
 90 103 133 50 49
    82 59 118 48
  
150         
 83 51 148 60 59
    96 86 166 57
  
180         
 76 81 82 36 44
    80 85 125 52
  
210         
 77 25 158 68 72
    60 28 134 75
Positive Control 3-Methylcholanthrene 
2.5       
 69 37 723 348 347*
    59 29 616 346

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 121 121 55 58
    81 81 128 52
    81 105 141 58
    85 93 174 68
Test Chemical Chlorpheniramine maleate 
110         
 85 72 112 44 49
    82 92 132 53
  
140         
 76 69 125 55 50
    77 78 102 44
  
170         
 79 48 162 68 63
    94 58 162 58
  
200         
 102 45 119 39 50
    62 45 113 61
  
230         
 75 29 114 51 48
    94 35 130 46
Positive Control 3-Methylcholanthrene 
2.5       
 52 14 722 463 504*
    43 16 702 544

Induced S9 Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58 89 135 78 82
    77 106 207 89
    76 110 192 84
    72 95 163 75
Test Chemical Chlorpheniramine maleate 
200         
 44 35 458 350 326*
    48 34 437 302
  
225         
 34 22 329 323 268*
    42 29 270 213
  
250         
 30 24 491 543 471*
    45 34 536 400
  
275         
 31 16 770 819 715*
    35 18 644 610
  
300         
 33 13 783 783 633*
    32 16 456 483
Positive Control 3-Methylcholanthrene 
2.5       
 38 27 415 366 408*
    37 25 498 451

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination