Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01877-0010-0000-6
Chemical Name:	Chlorpheniramine maleate
CASRN:	113-92-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	501757
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	97	129	46	45
			81	100	132	54
			94	104	100	36
			99	99	129	44
Test Chemical	Chlorpheniramine maleate	10	72	79	101	47	40
			96	88	96	33	40
		50	61	51	130	72	59
			66	33	94	47	59
		90	79r	13	152	64
			72	37	122	56
		130	85	20	169	66	62
			95	18	166	58	62
		170	75	8	145	64	63
			58	9	109	62	63
		210

Positive Control	Methyl Methane Sulfonate	15	43	11	462	360	360*
Positive Control			35	30	383	361	360*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	87	125	42	36
			95	119	89	31
			86	102	77	30
			89	92	105	39
Test Chemical	Chlorpheniramine maleate	75	61	24	93	51	55
			68	31	120	59	55
		150	99	22	110	37	41
			76	29	103	45	41
		175	65	9	86	44	42
			84	22	103	41	42
		200	84	14	61	24	27
			47	6	43	31	27
		225	84	10	61	24	27
			45	9	40	30	27
		250

		275

Positive Control	Methyl Methane Sulfonate	15	77	74	87	38	34
			83	79	75	30	34
	Methyl Methane Sulfonate	250	67	80	398	199	186*
			67	85	350	173	186*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	104	108	50	55
			62	94	109	59
			64	106	118	62
			77	97	118	51
Test Chemical	Chlorpheniramine maleate	90	99	105	158	53	48
			101	123	131	43	48
		120	90	103	133	50	49
			82	59	118	48	49
		150	83	51	148	60	59
			96	86	166	57	59
		180	76	81	82	36	44
			80	85	125	52	44
		210	77	25	158	68	72
			60	28	134	75	72
Positive Control	3-Methylcholanthrene	2.5	69	37	723	348	347*
Positive Control			59	29	616	346	347*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	121	121	55	58
			81	81	128	52
			81	105	141	58
			85	93	174	68
Test Chemical	Chlorpheniramine maleate	110	85	72	112	44	49
			82	92	132	53	49
		140	76	69	125	55	50
			77	78	102	44	50
		170	79	48	162	68	63
			94	58	162	58	63
		200	102	45	119	39	50
			62	45	113	61	50
		230	75	29	114	51	48
			94	35	130	46	48
Positive Control	3-Methylcholanthrene	2.5	52	14	722	463	504*
Positive Control			43	16	702	544	504*

Induced S9 Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	89	135	78	82
			77	106	207	89
			76	110	192	84
			72	95	163	75
Test Chemical	Chlorpheniramine maleate	200	44	35	458	350	326*
			48	34	437	302	326*
		225	34	22	329	323	268*
			42	29	270	213	268*
		250	30	24	491	543	471*
			45	34	536	400	471*
		275	31	16	770	819	715*
			35	18	644	610	715*
		300	33	13	783	783	633*
			32	16	456	483	633*
Positive Control	3-Methylcholanthrene	2.5	38	27	415	366	408*
Positive Control			37	25	498	451	408*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination