Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01879-0008-0000-5
Chemical Name:	Chlorpropamide
CASRN:	94-20-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	920836
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	100	101	37	29
			97	94	83	29
			95	95	71	25
			97	111	72	25
Test Chemical	Chlorpropamide	62.5	103	101	87	28	25
			117	114	74	21	25
		125	122r	100	92	25
			118	95	93	26
		250	107	97	96	30	27
			80	71	59	25	27
		500	85	105	58	23	26
			80	97	71	30	26
		1000	88	66	79	30	27
			107	71	78	24	27
Positive Control	Methyl Methane Sulfonate	15	65	44	230	118	109*
			63	38	188	99	109*
	Methyl Methane Sulfonate	250	91	79	395	145	139*
			87	79	346	133	139*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	100	96	41	35
			73	103	72	33
			69	85	73	35
			86	112	75	29
Test Chemical	Chlorpropamide	200	85	99	64	25	29
			88	101	88	34	29
		400	81	91	68	28	39
			71	77	107	50	39
		800	73	79	75	34	33
			70	62	66	32	33
		1200	95	71	78	27	30
			66	66	63	32	30
		1600	71	55	97	45	45
			77	45	102	44	45
Positive Control	Methyl Methane Sulfonate	15	50	36	124	83	84*
			50	39	128	85	84*
	Methyl Methane Sulfonate	250	71	66	363	170	187*
			76	68	467	204	187*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	99	126	55	54
			81	101	118	49
			76	97	129	57
			98	102	166	56
Test Chemical	Chlorpropamide	200	68	93	127	63	58
			72	94	117	54	58
		400	74	99	131	59	57
			68	86	112	55	57
		800	73	95	100	46	48
			63	84	97	51	48
		1200	74	80	115	52	50
			74	84	109	49	50
		1600	77	44	144	63	62
			56	35	103	61	62
Positive Control	3-Methylcholanthrene	2.5	50	41	554	367	366*
Positive Control			52	39	563	364	366*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	106	116	58	81
			59	99	140	79
			67	103	174	87
			60	92	175	98
Test Chemical	Chlorpropamide	100	76	120	104	46	42
			85	123	97	38	42
		200	69	122	79	38	48
			66	109	114	57	48
		400	64	72	94	49	46
			71	98	92	43	46
		800	92	88	144	52	55
			78	60	137	59	55
		1200	54	77	131	82	85
			55	21	147	89	85
		1600

Positive Control	3-Methylcholanthrene	2.5	18	6	256	483	651*
Positive Control			11	4	258	819	651*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination