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Study Information
Study Attribute Value
CEBS Accession Number: 002-01879-0008-0000-5
Chemical Name: Chlorpropamide
CASRN: 94-20-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 920836
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 100 101 37 29
    97 94 83 29
    95 95 71 25
    97 111 72 25
Test Chemical Chlorpropamide 
62.5       
 103 101 87 28 25
    117 114 74 21
  
125         
 122r 100 92 25
    118 95 93 26
  
250         
 107 97 96 30 27
    80 71 59 25
  
500         
 85 105 58 23 26
    80 97 71 30
  
1000         
 88 66 79 30 27
    107 71 78 24
Positive Control Methyl Methane Sulfonate 
15         
 65 44 230 118 109*
    63 38 188 99
Methyl Methane Sulfonate 
250         
 91 79 395 145 139*
    87 79 346 133

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 100 96 41 35
    73 103 72 33
    69 85 73 35
    86 112 75 29
Test Chemical Chlorpropamide 
200         
 85 99 64 25 29
    88 101 88 34
  
400         
 81 91 68 28 39
    71 77 107 50
  
800         
 73 79 75 34 33
    70 62 66 32
  
1200         
 95 71 78 27 30
    66 66 63 32
  
1600         
 71 55 97 45 45
    77 45 102 44
Positive Control Methyl Methane Sulfonate 
15         
 50 36 124 83 84*
    50 39 128 85
Methyl Methane Sulfonate 
250         
 71 66 363 170 187*
    76 68 467 204

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 99 126 55 54
    81 101 118 49
    76 97 129 57
    98 102 166 56
Test Chemical Chlorpropamide 
200         
 68 93 127 63 58
    72 94 117 54
  
400         
 74 99 131 59 57
    68 86 112 55
  
800         
 73 95 100 46 48
    63 84 97 51
  
1200         
 74 80 115 52 50
    74 84 109 49
  
1600         
 77 44 144 63 62
    56 35 103 61
Positive Control 3-Methylcholanthrene 
2.5       
 50 41 554 367 366*
    52 39 563 364

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 106 116 58 81
    59 99 140 79
    67 103 174 87
    60 92 175 98
Test Chemical Chlorpropamide 
100         
 76 120 104 46 42
    85 123 97 38
  
200         
 69 122 79 38 48
    66 109 114 57
  
400         
 64 72 94 49 46
    71 98 92 43
  
800         
 92 88 144 52 55
    78 60 137 59
  
1200         
 54 77 131 82 85
    55 21 147 89
  
1600         
   
Positive Control 3-Methylcholanthrene 
2.5       
 18 6 256 483 651*
    11 4 258 819

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination