Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01889-0011-0000-0
Chemical Name:	Cinnamyl anthranilate
CASRN:	87-29-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	668584
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	54	101	34	33
			107	120	119	37
			101	126	82	27
Test Chemical	Cinnamyl anthranilate	1.95	97	118	92	32	32
			99	105	93	31
			103	92	103	33
		3.9	90	106	78	29	27
			102	125	73	24
			97	120	81	28
		7.8	103	137	76	25	24
			97	125	73	25
			99	108	69	23
		15.6	96#	107	90	31	28
			104	106	77	25
			98	105	81	28
		31.3	99	96	85	29	31
			102#	71	101	33
			104	61	98	31
		62.5


Positive Control	Ethyl Methane Sulfonate	250	98#	62	1107	376	396*
			99#	75	1132	381
			77	67	1001	431

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	117#	141	69	20	24
			57	73	55	32
			63	86	40	21
Test Chemical	Cinnamyl anthranilate	5	92	105	60	22	20
			84	93	44	17
			91	112	55	20
		10	67	90	34	17	19
			69	93	31	15
			63#	108	46.5	25
		20	55#	58	36	22	20
			56	66	30	18
			64	79	37	19
		30	86	59	54	21	23
			73	70	56	26	23
		40	45	19	51	38	26
			66#	36	45	23
			73	42	40	18
		50	53#	7	45	28	37
			57#	19	78	46	37
		60


Positive Control	Ethyl Methane Sulfonate	250	34#	27	840	830	601*
			104#	110	1440	462
			87	76	1331	512

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105#	87	92	29	29
			87	92	82	31
			96#	100	86	30
			92	122	74	27
Test Chemical	Cinnamyl anthranilate	3	72	44	82	38	33
			84	67	83	33
			89	63	77	29
		6	82	40	84	34	35
			87	42	109	42
			85	38	72	28
		8	67	32	69	34	34
			72	32	86	40
			88	32	72	27
		10	85	19	117	46	44*
			91	28	121	44
			88	28	113	43
		15	99	25	100	34	40
			80	17	113	47
			94	24	108	38
		20	79	14	115	49	53*
			66	6	143	72
			103	12	116	38
Positive Control	3-Methylcholanthrene	3.5	104	84	457	147	140*
			112#	62	410	122
			99	65	443	150

Non-Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106	115	96	30	45
			96	95	132	46
			104	122	177	56
			71	68	100	47
Test Chemical	Cinnamyl anthranilate	2.5	66	49	106	53	50
			66	39	99	50
			69	59	95	46
		5	59#	23	77	44	54
			68	35	119	58
			79	28	140	59
		10	113	29	166	49	61
			99	46	185	62
			89	22	194	73
		15	59	4	130	73	81*
			62	9	181	98
			91#	7	199	73
		20	71	8	189	89


		25


Positive Control	3-Methylcholanthrene	3.5	87	56	421	162	156*
			86	41	376	147
			98	57	469	160

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106	74	76	24	27
			109	89	70	21
			115	130	118	34
			104	107	89	28
Test Chemical	Cinnamyl anthranilate	0.49	86	67	118	46	40
			80	72	81	34	40
		0.98	104#	80	85.5	27	31
			85	53	86	34
			81	55	78	32
		1.95	93#	46	106	38	44*
			90	66	136	50	44*
		3.9	89#	33	115	43	36
			96	34	95	33
			108#	38	100.5	31
		7.8	99#	12	205	69	60*
			86	29	130	50	60*
		15.6	73	10	143	65	81*
			66#	7	193.5	97	81*
		31.3


Positive Control	3-Methylcholanthrene	2.5	61	26	367	202	232*
			54#	21	384	237
			63	45	486	256

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102#	107	327	107	115
			89	89	321	121
			92#	103	325.5	118
Test Chemical	Cinnamyl anthranilate	0.49	63	93	205	109	126
			67#	70	296	147
			69	104	250	120
		0.98	96	81	431	150	139
			75#	60	286.5	127
			123r	122	471	127
		1.95	84	99	265	106	123
			65	63	243	125
			80	44	329	138
		3.9	86	50	399	154	132
			84	43	319	126
			104#	35	361.5	116
		7.8	84	25	542	215	207*
			93#	21	558	200	207*
		15.6


Positive Control	3-Methylcholanthrene	2.5	46	20	612	448	546*
Positive Control			49#	25	946	644	546*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96#	104	253	88	84
			86	89	218	85
			92#	106	220.5	80
Test Chemical	Cinnamyl anthranilate	0.49	60	92	155	86	100
			64#	69	224	117
			65	100	189	97
		0.98	93	81	313	112	104
			123r	125	340	92
			71#	59	205.5	96
		1.95	81	98	202	84	91
			79	46	235	99
			62	63	167	90
		3.9	101#	35	241.5	80	92
			82	43	222	90
			84	50	265	105
		7.8	82	25	361	147	134
			92#	22	334.5	122	134
		15.6


Positive Control	3-Methylcholanthrene	2.5	47#	24	616	442	382*
Positive Control			40	19	390	322	382*

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113#	100	238.5	70	63
			117	121	185	53
			108#	87	216	67
			88#	92	160.5	61
Test Chemical	Cinnamyl anthranilate	1	89	91	168	63
		2	90#	85	132	49	67
			103#	84	231	75
			76	93	176	77
		4	89#	41	256.5	97
		6	79#	38	222	94	85
			82	19	188	77	85
		8	93	21	234	84	97*
			76	10	275	121
			84#	19	215	85
		10	107	19	206	64	89
			84#	9	232.5	93
			77#	8	253.5	109
Positive Control	3-Methylcholanthrene	2.5	122r	59	816	224	247*
			95#	49	663	233
			94#	58	732	260

Induced S9 Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113#	100	186	55	46
			117	121	137	39
			108#	87	153	47
			88#	92	114	43
Test Chemical	Cinnamyl anthranilate	1	89	91	124	46
		2	90#	85	88.5	33	46
			103#	84	153	49
			76	93	127	55
		4	89#	41	169.5	64
		6	82	19	136	55	58
			79#	38	143	60	58
		8	84#	19	141	56	66
			93	21	152	54
			76	10	196	86
		10	77#	8	163.5	70	61
			107	19	142	44
			84#	9	171	68
Positive Control	3-Methylcholanthrene	2.5	122r	59	682.5	187	189*
			95#	49	513	180
			94#	58	556.5	198

Induced S9 Trial: 6 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89#	104	97	36	41
			100	90	118	39
			104	118	123	40
			98	88	143	49
Test Chemical	Cinnamyl anthranilate	1	72	67	93	43	44
			84	86	113	45
			72#	63	93	43
		2	91	74	98	36	35
			88	89	82	31
			99	88	110	37
		4	107#	69	140	44	44
			75#	61	94	42
			85	61	118	46
		6	80	54	97	41	35
			103#	57	93	30
			97#	60	99	34
		8	88	30	135	51	44
			111	38	146	44
			112	53	122	36
		10	107	35	131	41	46
			77	29	142	62
			103	41	106	34
Positive Control	3-Methylcholanthrene	2.5	96	58	791	275	287*
			80	58	687	285
			75	52	681	301

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination