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Study Information
Study Attribute Value
CEBS Accession Number: 002-01889-0011-0000-0
Chemical Name: Cinnamyl anthranilate
CASRN: 87-29-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 668584
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 54 101 34 33
    107 120 119 37
    101 126 82 27
Test Chemical Cinnamyl anthranilate 
1.95      
 97 118 92 32 32
    99 105 93 31
    103 92 103 33
  
3.9       
 90 106 78 29 27
    102 125 73 24
    97 120 81 28
  
7.8       
 103 137 76 25 24
    97 125 73 25
    99 108 69 23
  
15.6       
 96# 107 90 31 28
    104 106 77 25
    98 105 81 28
  
31.3       
 99 96 85 29 31
    102# 71 101 33
    104 61 98 31
  
62.5       
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 98# 62 1107 376 396*
    99# 75 1132 381
    77 67 1001 431

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 117# 141 69 20 24
    57 73 55 32
    63 86 40 21
Test Chemical Cinnamyl anthranilate 
5         
 92 105 60 22 20
    84 93 44 17
    91 112 55 20
  
10         
 67 90 34 17 19
    69 93 31 15
    63# 108 46.5 25
  
20         
 55# 58 36 22 20
    56 66 30 18
    64 79 37 19
  
30         
 86 59 54 21 23
    73 70 56 26
  
40         
 45 19 51 38 26
    66# 36 45 23
    73 42 40 18
  
50         
 53# 7 45 28 37
    57# 19 78 46
  
60         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 34# 27 840 830 601*
    104# 110 1440 462
    87 76 1331 512

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105# 87 92 29 29
    87 92 82 31
    96# 100 86 30
    92 122 74 27
Test Chemical Cinnamyl anthranilate 
3         
 72 44 82 38 33
    84 67 83 33
    89 63 77 29
  
6         
 82 40 84 34 35
    87 42 109 42
    85 38 72 28
  
8         
 67 32 69 34 34
    72 32 86 40
    88 32 72 27
  
10         
 85 19 117 46 44*
    91 28 121 44
    88 28 113 43
  
15         
 99 25 100 34 40
    80 17 113 47
    94 24 108 38
  
20         
 79 14 115 49 53*
    66 6 143 72
    103 12 116 38
Positive Control 3-Methylcholanthrene 
3.5       
 104 84 457 147 140*
    112# 62 410 122
    99 65 443 150

Non-Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 115 96 30 45
    96 95 132 46
    104 122 177 56
    71 68 100 47
Test Chemical Cinnamyl anthranilate 
2.5       
 66 49 106 53 50
    66 39 99 50
    69 59 95 46
  
5         
 59# 23 77 44 54
    68 35 119 58
    79 28 140 59
  
10         
 113 29 166 49 61
    99 46 185 62
    89 22 194 73
  
15         
 59 4 130 73 81*
    62 9 181 98
    91# 7 199 73
  
20         
 71 8 189 89
   
   
  
25         
   
   
Positive Control 3-Methylcholanthrene 
3.5       
 87 56 421 162 156*
    86 41 376 147
    98 57 469 160

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 74 76 24 27
    109 89 70 21
    115 130 118 34
    104 107 89 28
Test Chemical Cinnamyl anthranilate 
 0.49      
 86 67 118 46 40
    80 72 81 34
  
 0.98      
 104# 80 85.5 27 31
    85 53 86 34
    81 55 78 32
  
1.95      
 93# 46 106 38 44*
    90 66 136 50
  
3.9       
 89# 33 115 43 36
    96 34 95 33
    108# 38 100.5 31
  
7.8       
 99# 12 205 69 60*
    86 29 130 50
  
15.6       
 73 10 143 65 81*
    66# 7 193.5 97
  
31.3       
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 61 26 367 202 232*
    54# 21 384 237
    63 45 486 256

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 102# 107 327 107 115
    89 89 321 121
    92# 103 325.5 118
Test Chemical Cinnamyl anthranilate 
 0.49      
 63 93 205 109 126
    67# 70 296 147
    69 104 250 120
  
 0.98      
 96 81 431 150 139
    75# 60 286.5 127
    123r 122 471 127
  
1.95      
 84 99 265 106 123
    65 63 243 125
    80 44 329 138
  
3.9       
 86 50 399 154 132
    84 43 319 126
    104# 35 361.5 116
  
7.8       
 84 25 542 215 207*
    93# 21 558 200
  
15.6       
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 46 20 612 448 546*
    49# 25 946 644

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96# 104 253 88 84
    86 89 218 85
    92# 106 220.5 80
Test Chemical Cinnamyl anthranilate 
 0.49      
 60 92 155 86 100
    64# 69 224 117
    65 100 189 97
  
 0.98      
 93 81 313 112 104
    123r 125 340 92
    71# 59 205.5 96
  
1.95      
 81 98 202 84 91
    79 46 235 99
    62 63 167 90
  
3.9       
 101# 35 241.5 80 92
    82 43 222 90
    84 50 265 105
  
7.8       
 82 25 361 147 134
    92# 22 334.5 122
  
15.6       
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 47# 24 616 442 382*
    40 19 390 322

Induced S9 Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113# 100 238.5 70 63
    117 121 185 53
    108# 87 216 67
    88# 92 160.5 61
Test Chemical Cinnamyl anthranilate 
1         
 89 91 168 63
  
2         
 90# 85 132 49 67
    103# 84 231 75
    76 93 176 77
  
4         
 89# 41 256.5 97
  
6         
 79# 38 222 94 85
    82 19 188 77
  
8         
 93 21 234 84 97*
    76 10 275 121
    84# 19 215 85
  
10         
 107 19 206 64 89
    84# 9 232.5 93
    77# 8 253.5 109
Positive Control 3-Methylcholanthrene 
2.5       
 122r 59 816 224 247*
    95# 49 663 233
    94# 58 732 260

Induced S9 Trial: 5 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113# 100 186 55 46
    117 121 137 39
    108# 87 153 47
    88# 92 114 43
Test Chemical Cinnamyl anthranilate 
1         
 89 91 124 46
  
2         
 90# 85 88.5 33 46
    103# 84 153 49
    76 93 127 55
  
4         
 89# 41 169.5 64
  
6         
 82 19 136 55 58
    79# 38 143 60
  
8         
 84# 19 141 56 66
    93 21 152 54
    76 10 196 86
  
10         
 77# 8 163.5 70 61
    107 19 142 44
    84# 9 171 68
Positive Control 3-Methylcholanthrene 
2.5       
 122r 59 682.5 187 189*
    95# 49 513 180
    94# 58 556.5 198

Induced S9 Trial: 6 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89# 104 97 36 41
    100 90 118 39
    104 118 123 40
    98 88 143 49
Test Chemical Cinnamyl anthranilate 
1         
 72 67 93 43 44
    84 86 113 45
    72# 63 93 43
  
2         
 91 74 98 36 35
    88 89 82 31
    99 88 110 37
  
4         
 107# 69 140 44 44
    75# 61 94 42
    85 61 118 46
  
6         
 80 54 97 41 35
    103# 57 93 30
    97# 60 99 34
  
8         
 88 30 135 51 44
    111 38 146 44
    112 53 122 36
  
10         
 107 35 131 41 46
    77 29 142 62
    103 41 106 34
Positive Control 3-Methylcholanthrene 
2.5       
 96 58 791 275 287*
    80 58 687 285
    75 52 681 301

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination