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Study Information
Study Attribute Value
CEBS Accession Number: 002-01890-0001-0000-1
Chemical Name: Citral
CASRN: 5392-40-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 881385_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2   
No Activation  3   
No Activation  4  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9   
Induced Rat Liver S9  4_S9   
Induced Rat Liver S9  5_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 12/29/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 393 0.379 7.86 26.0 0.000
Negative (Not Specified) 
0         
 50 1039 405 0.390 8.10 26.0 2.756
Test Chemical Citral 
 0.289     
 50 1036 476 0.459 9.52 26.0 21.120 
 0.868     
 50 1041 480 0.461 9.60 26.0 21.551 
2.89      
 50 1025 570 0.556 11.40 26.0 46.595 
8.86      
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
1.5       
 50 1044 703 0.673 14.06 26.0 77.510
Mitomycin-C 
10         
 5 105 229 2.181 45.80 26.0 474.928
Trend: 5.629        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 01/10/1984    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1035 427 0.413 8.54 26.0 0.000
Test Chemical Citral 
17.4       
 50 1036 501 0.484 10.02 26.0 17.217
Positive Control Mitomycin-C 
 0.0015    
 50 1040 792 0.762 15.84 26.0 84.588
Mitomycin-C 
 0.01      
 5 104 270 2.596 54.00 26.0 529.279
Trend: 0.000        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 01/24/1984    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 458 0.437 9.16 26.0 0.000
Test Chemical Citral 
1         
 50 1043 872 0.836 17.44 26.0 91.123 
5.1       
 0 0 0 0.000 0.00 0.0 N/A 
7.5       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1040 700 0.673 14.00 26.0 53.867
Mitomycin-C 
 0.01      
 5 104 145 1.394 29.00 26.0 218.725
Trend: 11.422        
Probability: 0.000        

Trial #: 4    Activation: No Activation    Date: 02/15/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 494 0.472 9.88 26.0 0.000
Test Chemical Citral 
2.5       
 50 1046 512 0.489 10.24 26.0 3.644 
5         
 50 1043 544 0.522 10.88 26.0 10.438 
7.5       
 50 1047 720 0.688 14.40 26.0 45.610 
10         
 50 1026 789 0.769 15.78 33.0 62.830 
20         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0015    
 50 1046 692 0.662 13.84 26.0 40.081
Mitomycin-C 
 0.01      
 5 104 222 2.135 44.40 26.0 351.985
Trend: 10.015        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/29/1983    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1047 423 0.404 8.46 26.0 -10.306
Dimethyl Sulfoxide 
0         
 50 1039 468 0.450 9.36 26.0 0.000
Test Chemical Citral 
 0.87      
 50 1042 509 0.488 10.18 26.0 8.448 
2.89      
 50 1035 546 0.528 10.92 26.0 17.118 
8.68      
 50 1035 693 0.670 13.86 26.0 48.649 
28.9       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1038 650 0.626 13.00 26.0 39.023
Cyclophosphamide 
2         
 5 105 147 1.400 29.40 26.0 210.812
Trend: 6.820        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/10/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 627 0.601 12.54 26.0 0.000
Test Chemical Citral 
30         
 50 1044 607 0.581 12.14 26.0 -3.190 
35         
 50 1043 653 0.626 13.06 26.0 4.247 
40         
 50 1027 604 0.588 12.08 26.0 -2.074
Positive Control Cyclophosphamide 
 0.4       
 50 1031 802 0.778 16.04 26.0 29.524
Cyclophosphamide 
2         
 5 104 252 2.423 50.40 26.0 303.460
Trend: 0.109        
Probability: 0.457        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 01/24/1984    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1005 494 0.492 9.88 26.0 0.000
Test Chemical Citral 
10         
 50 1031 851 0.825 17.02 26.0 67.923 
20         
 0 0 0 0.000 0.00 0.0 N/A 
30         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1034 683 0.661 13.66 26.0 34.381
Cyclophosphamide 
2         
 5 105 179 1.705 35.80 26.0 246.819
Trend: 9.266        
Probability: 0.000        

Trial #: 4_S9    Activation: Induced Rat Liver S9    Date: 02/15/1984    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1038 618 0.595 12.36 26.0 0.000
Test Chemical Citral 
5         
 50 1044 698 0.669 13.96 26.0 12.296 
7.5       
 50 1042 650 0.624 13.00 26.0 4.774 
10         
 50 1043 636 0.610 12.72 33.0 2.419 
15         
 50 1044 679 0.650 13.58 33.0 9.239 
20         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1046 851 0.814 17.02 26.0 36.649
Cyclophosphamide 
2         
 5 103 194 1.883 38.80 26.0 216.354
Trend: 0.665        
Probability: 0.253        

Trial #: 5_S9    Activation: Induced Rat Liver S9    Date: 04/10/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1032 478 0.463 9.56 26.0 0.000
Test Chemical Citral 
15.1       
 50 1024 651 0.636 13.02 26.0 37.256 
20.1       
 50 1026 771 0.751 15.42 34.0 62.240 
25.2       
 50 1015 739 0.728 14.78 34.0 57.192 
30.2       
 25 508 402 0.791 16.08 34.0 70.850 
40.2       
 25 510 387 0.759 15.48 34.0 63.830
Positive Control Cyclophosphamide 
 0.4       
 50 1025 764 0.745 15.28 26.0 60.924
Cyclophosphamide 
2         
 5 101 135 1.337 27.00 26.0 188.579
Trend: 8.586        
Probability: 0.000