Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01900-0018-0000-1
Chemical Name:	Coconut oil acid diethanolamine condensate
CASRN:	68603-42-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	876640
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	72	81	134	62	50
			64	123	83	44
			76	101	108	47
			74	94	103	47
Test Chemical	Coconut oil acid diethanolamine condensate	1.25	83	90	118	47	49

			63	67	95	50
		2.5	77	77	114	49	50
			53	65	72	46
			75	74	128	57
		5	70	54	114	55	52
			57	47	97	57
			61	63	81	44
		6	64	46	99	52	52
			70	46	89	42
			69	53	126	61
		10	72	15	139	65	70
			54	9	135	84
			61	84	113	62
		12


Positive Control	Methyl Methane Sulfonate	5	49	39	769	525	523*
			60	60	704	390
			33	38	644	654

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	108	106	38	12	17
			115	110	60	17
			92	91	62	22
			93	94	45	16
Test Chemical	Coconut oil acid diethanolamine condensate	4	96	113	42	15	18
			100	99	61	20
			91	90	48	18
		5	75	69	47	21	19
			101	89	52	17
			85	87	49	19
		6	91	76	72	26	21
			93	82	44	16
			90	99	53	20
		8	86	73	54	21	28*
			78	65	61	26
			74	63	82	37
		10	92	50	82	30	25
			100	58	74	25
			114	64	70	21
		12	75	50	65	29	26
			99	27	54	18
			77	45	68	30
Positive Control	Methyl Methane Sulfonate	5	89	77	182	68	74*
			119	101	271	76
			90	72	213	79

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	114	135	85	25	29
			111	125	73	22
			115	74	91	26
			92	66	119	43
Test Chemical	Coconut oil acid diethanolamine condensate	1.5	103	135	93	30	27
			102	113	77	25
			116	200	87	25
		3	87	113	108	41	34
			114	126	99	29
			98	114	96	33
		6	112	92	86	26	36
			92	83	104	38
			83	84	110	44
		8	111	34	83	25	38
			108	45	178	55
			115	37	116	34
		10	94	20	87	31	31
			107#	76	109	34
			101	25	85	28
Positive Control	Methyl Methane Sulfonate	5	51#	33	868	564	420*
			93	52	698	252
			43	27	574	445

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	102	129	111	36	57
			71	102	179	84
			83	93	136	54
			84	76	138	55
Test Chemical	Coconut oil acid diethanolamine condensate	1.25	50#	97	116	77	75
			73	89	148	68
			58	73	137	79
		2.5	83#	105	141	56	59
			74	117	138	62
			78	117	134	57
		5	69	107	92	45	33
			91#	135	61	22

		10	54	14	126	78	61
			92#	27	121	44	61
		15


Positive Control	3-Methylcholanthrene	2.5	80	45	822	344	371*
			60	68	680	377
			66	57	781	392

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	82	99	141	58	58
			85	78	144	56
			91	106	183	67
			78	117	119	51
Test Chemical	Coconut oil acid diethanolamine condensate	4	88	137	184	70	71
			103	144	206	67
			95	121	215	75
		5	76	123	166	73	67
			75	143	157	70
			80	140	141	59
		6	72	113	181	84	74
			83	131	170	69
			67	73	141	70
		8	96	89	215	75	71
			108	118	182	56
			76	92	186	82
		10	105	81	194	62	68
			75	79	137	61
			83	81	202	81
		12	93	83	166	60	67
			78	65	189	81
			81	88	147	60
Positive Control	3-Methylcholanthrene	2.5	51	26	570	376	458*
			39	15	626	542
			45	18	613	456

Induced S9 Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	103	48	60	19	34
			88	98	113	43
			104	140	116	37
			98	114	102	35
Test Chemical	Coconut oil acid diethanolamine condensate	6	73	85	106	48	48
			95	109	155	55
			105	116	127	40
		8	96	115	143	50	43
			107	111	101	32
			71	84	101	48
		10	105	127	123	39	38
			98	88	99	34
			95	85	117	41
		12	102	105	121	40	36
			105	85	97	31
			90	117	98	36
		15	87	45	101	39	42
			97	63	108	37
			91	47	137	50
		20	104	92	110	35	36
			93	131	118	42
			107	135	97	30
Positive Control	3-Methylcholanthrene	2.5	90	73	457	169	185*
			105	69	605	192
			100	76	578	194

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	77	96	101	44	57
			78	126	129	55
			71	32	146	68
			82	145	147	60
Test Chemical	Coconut oil acid diethanolamine condensate	5	66	113	113	57	49
			65	89	93	48
			67	78	86	43
		10	79	100	153	65	69
			62	94	137	74
			72	88	150	70
		15	66	108	115	58	57
			79	103	147	62
			66	100	100	51
		20	58	89	115	66	63
			67	76	117	58
			58	74	114	66
		30	57	78	93	55	56
			72	95	122	57
			53	38	91	57
		40	62	18	128	69	70
			63	17	136	72

		50


Positive Control	3-Methylcholanthrene	2.5	84	69	447	177	251*
			60	44	442	246
			60	25	598	330

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination