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Study Information
Study Attribute Value
CEBS Accession Number: 002-01901-0002-0000-5
Chemical Name: Codeine phosphate
CASRN: 52-28-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 039151_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Negative 
No Activation  4  Test Failure 

Trial #:1   Activation: No Activation    Date: 03/08/1984    Harvest Time: 20.6 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
2500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
3000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
3500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.025     
 100 16 0.160 14.0 5 0.050 5.0 11 0.110 10.0 0 0.000 0.0
Mitomycin-C 
 0.0625    
 25 6 0.240 24.0 1 0.040 4.0 4 0.160 16.0 1 0.040 4.0
Trend: -0.485 -1.210 0.015  
Probability: 0.686 0.887 0.494  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/27/1984    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
9000         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
9500         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
10000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 100 7 0.070 7.0 4 0.040 4.0 3 0.030 3.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 8 0.320 24.0 2 0.080 8.0 6 0.240 20.0 0 0.000 0.0
Trend: 0.684 1.334 0.289  
Probability: 0.247 0.091 0.386  


Trial #:2   Activation: No Activation    Date: 03/08/1984    Harvest Time: 20.6 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Medium 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
3500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
4000         
 100 4 0.040 2.0 0 0.000 0.0 4 0.040 2.0 0 0.000 0.0 
4500         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
5000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.025     
 100 9 0.090 9.0 3 0.030 3.0 6 0.060 6.0 0 0.000 0.0
Mitomycin-C 
 0.0625    
 25 5 0.200 20.0 3 0.120 12.0 2 0.080 8.0 0 0.000 0.0
Trend: 0.248 1.263 -0.449  
Probability: 0.402 0.103 0.673  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 02/27/1984    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Medium 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
9000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
9500         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
10000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 6 0.240 24.0 2 0.080 8.0 4 0.160 16.0 0 0.000 0.0
Trend: 0.610 0.624 0.275  
Probability: 0.271 0.266 0.391  


Trial #:3   Activation: No Activation    Date: 02/27/1984    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
2000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2500         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
3000         
 100 4 0.040 3.0 0 0.000 0.0 4 0.040 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0026    
 100 15 0.150 14.0 6 0.060 5.0 8 0.080 8.0 1 0.010 1.0
Mitomycin-C 
 0.062     
 25 13 0.520 40.0 5 0.200 20.0 8 0.320 24.0 0 0.000 0.0
Trend: 0.610 -0.192 0.852  
Probability: 0.271 0.576 0.197  


Trial #:4   Activation: No Activation    Date: 02/27/1984    Harvest Time: 20.5 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Codeine phosphate 
3000         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0025    
 100 15 0.150 11.0 4 0.040 4.0 7 0.070 6.0 4 0.040 1.0
Mitomycin-C 
 0.0625    
 25 9 0.360 28.0 1 0.040 4.0 8 0.320 28.0 0 0.000 0.0
Trend: 0.000 0.000 0.000  
Probability: 0.000 0.000 0.000