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Study Information
Study Attribute Value
CEBS Accession Number: 002-01911-0001-0000-5
Chemical Name: Croton oil
CASRN: 8001-28-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 356212_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1   
No Activation  2   
No Activation  3  Negative 
Induced Rat Liver S9  1_S9   
Induced Rat Liver S9  2_S9   
Induced Rat Liver S9  3_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 02/12/1982    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 432 0.413 8.64 25.5 0.000
Test Chemical Croton oil 
89.6       
 50 1036 463 0.447 9.26 25.5 8.314 
299         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 25 524 555 1.059 22.20 25.5 156.699
Trend: 1.194        
Probability: 0.116        

Trial #: 2    Activation: No Activation    Date: 02/17/1982    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 499 0.478 9.98 25.5 0.000
Test Chemical Croton oil 
278         
 50 1045 496 0.475 9.92 25.5 -0.696
Positive Control Mitomycin-C 
 0.005     
 25 521 756 1.451 30.24 25.5 203.588
Trend: 0.000        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 03/09/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1037 408 0.393 8.16 25.5 0.000
Test Chemical Croton oil 
349         
 50 1038 439 0.423 8.78 25.5 7.494 
403         
 50 1030 434 0.421 8.68 25.5 7.095 
421         
 50 1026 474 0.462 9.48 25.5 17.422
Positive Control Mitomycin-C 
 0.005     
 25 517 471 0.911 18.84 25.5 131.552
Trend: 2.057        
Probability: 0.020        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/12/1982    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 468 0.448 9.36 25.5 0.000
Test Chemical Croton oil 
89.6       
 50 1047 481 0.459 9.62 25.5 2.483 
299         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
2         
 25 521 869 1.668 34.76 25.5 272.080
Trend: 0.378        
Probability: 0.353        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/17/1982    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 511 0.489 10.22 25.5 0.000
Test Chemical Croton oil 
278         
 50 1032 528 0.512 10.56 25.5 4.528
Positive Control Cyclophosphamide 
1.5       
 25 524 729 1.391 29.16 25.5 184.234
Trend: 0.000        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 03/18/1982    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1052 353 0.336 7.06 26.0 0.000
Test Chemical Croton oil 
251         
 50 1044 391 0.375 7.82 26.0 11.614 
278         
 50 1031 415 0.403 8.30 26.0 19.958 
278         
 50 1032 434 0.421 8.68 30.0 25.329 
296         
 50 1031 411 0.399 8.22 26.0 18.802 
296         
 50 1043 397 0.381 7.94 30.0 13.435
Positive Control Cyclophosphamide 
1.5       
 25 481 523 1.087 20.92 26.0 224.039
Trend: 2.402        
Probability: 0.008