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Study Information
Study Attribute Value
CEBS Accession Number: 002-01911-0002-0000-6
Chemical Name: Croton oil
CASRN: 8001-28-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 356212_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 04/07/1982    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 11 0.110 9.0 2 0.020 2.0 8 0.080 7.0 1 0.010 1.0
Dimethyl Sulfoxide 
0         
 100 6 0.060 6.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: Croton oil 
699         
 100 5 0.050 3.0 0 0.000 0.0 5 0.050 3.0 0 0.000 0.0 
753         
 100 5 0.050 5.0 2 0.020 2.0 2 0.020 2.0 1 0.010 1.0 
797         
 100 8 0.080 8.0 2 0.020 2.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 50 13 0.260 20.0 4 0.080 8.0 9 0.180 14.0 0 0.000 0.0
Trend: 0.760 -0.157 0.911  
Probability: 0.224 0.562 0.181  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/07/1982    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 6 0.060 6.0 1 0.010 1.0 5 0.050 5.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Croton oil 
350         
 100 7 0.070 6.0 2 0.020 2.0 5 0.050 4.0 0 0.000 0.0 
374         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
403         
 100 5 0.050 4.0 3 0.030 3.0 2 0.020 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 50 9 0.180 12.0 5 0.100 6.0 4 0.080 4.0 0 0.000 0.0
Trend: 0.459 0.873 -0.165  
Probability: 0.323 0.191 0.566  


Trial #:2   Activation: No Activation    Date: 03/20/1982    Harvest Time: 10.6 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 7 0.070 7.0 3 0.030 3.0 4 0.040 4.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 6 0.060 6.0 0 0.000 0.0 6 0.060 6.0 0 0.000 0.0
Test Chemical: Croton oil 
600         
 100 6 0.060 6.0 2 0.020 2.0 4 0.040 4.0 0 0.000 0.0 
699         
 100 6 0.060 5.0 1 0.010 1.0 5 0.050 4.0 0 0.000 0.0 
797         
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 50 8 0.160 10.0 4 0.080 6.0 4 0.080 6.0 0 0.000 0.0
Trend: -0.397 0.991 -0.994  
Probability: 0.654 0.161 0.840  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/20/1982    Harvest Time: 10.6 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 11 0.110 9.0 6 0.060 5.0 5 0.050 4.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 8 0.080 5.0 3 0.030 2.0 5 0.050 4.0 0 0.000 0.0
Test Chemical: Croton oil 
197         
 100 9 0.090 9.0 1 0.010 1.0 7 0.070 7.0 1 0.010 1.0 
296         
 100 6 0.060 4.0 3 0.030 2.0 3 0.030 3.0 0 0.000 0.0 
349         
 100 3 0.030 3.0 0 0.000 0.0 2 0.020 2.0 1 0.010 1.0 
403         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 2 0.040 2.0 1 0.020 1.0 1 0.020 1.0 0 0.000 0.0
Trend: -1.212 -0.824 -1.232  
Probability: 0.887 0.795 0.891