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Study Information
Study Attribute Value
CEBS Accession Number: 002-01922-0007-0000-3
Chemical Name: Cyclohexanone
CASRN: 108-94-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 910786
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 103 183 58 40
    89 88 90 34
    83 98 97 39
    88 111 77 29
Test Chemical Cyclohexanone 
312.5       
 58 63 131 76 70*
    80 70 154 64
  
625         
 44 52 95 71 76*
    61 71 147 80
  
1250         
 86 88 108 42 42
    69 78 85 41
  
2500         
 60 71 115 64
  
5000         
 71 73 122 57 62
    63 52 127 67
Positive Control Methyl Methane Sulfonate 
15         
 33 29 300 303 236*
    60 45 304 169

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 98 93 37 37
    117 92 112 32
    80 97 110 46
    99 112 100 34
Test Chemical Cyclohexanone 
1000         
 78 93 106 45 40
    78 73 84 36
  
2000         
 110 89 160 48 42
    83 78 88 36
  
3000         
 81 97 56 23 30
    78 84 88 38
  
4000         
 68 68 72 35 35
    92 79 96 35
  
5000         
 101 93 111 37 36
    97 78 101 35
Positive Control Methyl Methane Sulfonate 
15         
 29 23 343 394 375*
    30 29 323 355

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 61 108 63 35 37
    56 94 71 43
    53 99 52 33
    38r 83 32 28
Test Chemical Cyclohexanone 
1000         
 47 88 53 37 35
    67 117 65 33
  
2000         
 76 115 77 34 41
    52 104 75 48
  
3000         
 57 90 66 39 43
    40 83 57 47
  
4000         
 41 70 81 66 59
    38 66 60 53
  
5000         
 57 78 91 53 48
    60 107 79 44
Positive Control 3-Methylcholanthrene 
2.5       
 39 36 237 201 203*
    43 36 265 205

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 89 58 21 24
    86 113 57 22
    90 111 70 26
    82 87 68 28
Test Chemical Cyclohexanone 
2000         
 93 115 82 29 26
    78 85 53 23
  
3000         
 86 99 92 36 31
    82 68 64 26
  
4000         
 80 110 106 44 39
    87 107 86 33
  
5000         
 92 113 82 30 35
    84 94 102 41
Positive Control 3-Methylcholanthrene 
2.5       
 43 15 294 231 231*
    48 22 331 231

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination