Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01931-0032-0000-1
Chemical Name:	Cyclophosphamide
CASRN:	50-18-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G87007
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	116	114	84	24	28
Vehicle Control			87	86	83	32	28
Test Chemical	Cyclophosphamide	1573	94	84	182	65	62*
			98	85	172	58	62*
		1966	91	85	172	63	63*
			102	85	194	63	63*
		2458	138r	107	232	56
			115	125	212	62
		3072	123r	95	287	78
			104	95	214	69
		3840	105	66	293	93	90*
			112	78	289	86	90*
		4800	121r	94	358	99
			84	58	338	134
		6000	64	33	454	235
Positive Control	Ethyl Methane Sulfonate	500	1r	0	887	39422	584*
Positive Control			51	31	888	584	584*

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	117	311	142	161
Vehicle Control			63	83	337	180	161
Test Chemical	Cyclophosphamide	3.15	24	23	342	475	521*
			20	18	334	566	521*
		3.93	21	18	278	448
			10r	8	249	874
		4.92	7r	4	150	750
			5r	5	165	1138
		6.14	2r	1	76	1520
			2r	1	57	1140
		7.68	0r	0	7	1400
Positive Control	3-Methylcholanthrene	5	20	11	307	507	489*
Positive Control			19	8	261	470	489*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95	109	141	49	48
			82	108	135	55
			84	92	100	40
			75	91	109	48
Test Chemical	Cyclophosphamide	1.05	83	84	227	91
		1.31	62	56	168	91
		1.64	64	62	163	85	85*
			61	65	154	84	85*
		2.05	57	52	180	106	114*
			61	54	223	123	114*
		2.56	55	44	231	141	153*
			55	48	271	164	153*
		3.2	46	40	259	190	193*
			41	33	239	196	193*
		4	37	23	210	192	182*
			38	24	193	172	182*
		5	17	10	197	386	366*
			21	12	216	346	366*
Positive Control	3-Methylcholanthrene	5	40	26	484	403	374*
Positive Control			45	28	462	345	374*

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	121	138	54	55
			84	114	118	47
			79	64	135	57
			85	101	152	60
Test Chemical	Cyclophosphamide	2.52	49	52	369	250	227*
			57	68	348	204	227*
		3.15	62	69	211	113	109*
			69	66	184	88
			58	54	221	126
		3.93	67	66	195	97	192*
			40	36	276	228
			46	36	349	252
		4.92	39	28	427	368	388*
			39	29	415	352
			34	26	456	443
		6.14	19	10	423	762	621*
			23	13	371	534
			25	14	416	566
		7.68	15	7	207	455	688*
			10	6	281	921
			9r	4	196	769
		9.6	8r	3	119	529
			5r	2	111	694
			8r	3	180	735
		12	7r	2	117	600
			6r	2	83	488
			5r	2	110	710
Positive Control	3-Methylcholanthrene	5	49	36	524	359	316*
			56	44	490	292
			63	52	561	296

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination