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Study Information
Study Attribute Value
CEBS Accession Number: 002-01936-0001-0000-2
Chemical Name: D & C green no. 5
CASRN: 4403-90-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 764297_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 11/04/1987    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1028 433 0.421 8.66 26.0 0.000
Test Chemical D & C green no. 5 
16         
 50 1041 417 0.401 8.34 26.0 -4.898 
50         
 50 1041 455 0.437 9.10 26.0 3.769 
160         
 50 1033 461 0.446 9.22 26.0 5.951 
500         
 50 1037 577 0.556 11.54 26.0 32.100
Positive Control Mitomycin-C 
 0.0E-4    
 50 1030 554 0.538 11.08 26.0 27.696
Mitomycin-C 
 0.005     
 10 208 207 0.995 20.70 26.0 136.272
Trend: 4.788        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/15/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1043 445 0.427 8.90 26.0 0.000
Test Chemical D & C green no. 5 
300         
 50 1032 549 0.532 10.98 26.0 24.686 
500         
 50 1038 625 0.602 12.50 26.0 41.126 
750         
 50 1040 668 0.642 13.36 31.0 50.545 
1000         
 50 1049 672 0.641 13.44 31.0 50.147
Positive Control Mitomycin-C 
 0.0E-4    
 50 1045 559 0.535 11.18 26.0 25.378
Mitomycin-C 
 0.005     
 10 210 250 1.190 25.00 26.0 179.026
Trend: 7.452        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/04/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1050 446 0.425 8.92 26.0 0.000
Test Chemical D & C green no. 5 
50         
 50 1048 438 0.418 8.76 26.0 -1.606 
160         
 50 1049 454 0.433 9.08 26.0 1.891 
500         
 50 1043 394 0.378 7.88 26.0 -11.066 
1000         
 50 1050 502 0.478 10.04 26.0 12.556
Positive Control Cyclophosphamide 
 0.1       
 50 1050 556 0.530 11.12 26.0 24.664
Cyclophosphamide 
 0.6       
 10 210 220 1.048 22.00 26.0 146.637
Trend: 0.806        
Probability: 0.210