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Study Information
Study Attribute Value
CEBS Accession Number: 002-01940-0009-0000-5
Chemical Name: D & C red no. 9
CASRN: 5160-02-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 494246
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 107 63 25 26
    83 98 59 24
    81 92 67 28
    78# 103 62 27
Test Chemical D & C red no. 9 
1.25      
 73 67 60 28 34
    85 75 100 39
    78 83 82 35
  
2.5       
 91 71 89 33 31
    84 70 76 30
    105 79 95 30
  
5         
 105 98 97 31 26
    101 82 88 29
    98 115 53 18
  
7.5       
 104# 89 88 28 35
    91 78 114 42
    93 85 95 34
  
15         
 86# 77 69 27 26
    86 85 59 23
    86# 74 70.5 27
Positive Control Methyl Methane Sulfonate 
5         
 51 40 611 403 361*
    35 20 164 155
    40# 30 634 524

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 107 72 25 31
    87 91 85 33
    93 115 94 34
    88 87 88 34
Test Chemical D & C red no. 9 
1.25      
 91 83 86 32 32
    83 103 84 34
    76 83 69 30
  
2.5       
 96 85 109 38 34
    94 105 84 30
    94 94 96 34
  
5         
 96 86 119 41 33
    92 90 93 34
    99 111 70 24
  
7.5       
 96 94 137 48 44
    103 90 126 41
    109 88 141 43
  
10         
 99 71 119 40 44
    99 70 141 47
    104 95 139 44
  
15         
 121r 97 143 40 35
    113 96 112 33
    89 88 101 38
Positive Control Methyl Methane Sulfonate 
5         
 51 32 399 263 273*
    78 61 517 221
    44 24 439 335

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 101 76 26 27
    73 93 74 34
    87 105 64 25
    75 101 56 25
Test Chemical D & C red no. 9 
2         
 100 82 82 27 26
    92 79 82 30
    111 102 73 22
  
3         
 95 100 66 23 21
    92 94 57 21
    88 90 54 20
  
4         
 110 117 58 18 16
    99 90 54 18
    111 98 39 12
  
5         
 111# 93 107 32 26
    110 105 77 23
    105 97 66 21
  
6         
 116 76 72 21 26
    94 111 88 31
    105 105 78 25
Positive Control 3-Methylcholanthrene 
2.5       
 91 81 448 164 160*
    72 89 359 166
    99 78 450 152

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 111 106 33 34
    114 95 94 27
    121r 90 106 29
    107 94 130 41
Test Chemical D & C red no. 9 
2         
 104 61 146 47 40
    125r 93 117 31
    94 76 96 34
  
3         
 114 65 152 45 39
    116 80 121 35
    112 75 126 37
  
4         
 101 74 163 54 48
    102 78 130 42
    110 81 154 47
  
5         
 111 79 167 50 46
    112 83 156 46
    94 83 119 42
  
6         
 113 72 164 48 41
    117 76 108 31
    93 63 119 43
  
8         
 122r 94 145 40 41
    113 83 121 36
    110 60 154 47
Positive Control 3-Methylcholanthrene 
2.5       
 86# 22 815 315 393*
    53 19 622 392
    57 17 807 471

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination