Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01940-0009-0000-5 |
Chemical Name: | D & C red no. 9 |
CASRN: | 5160-02-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 494246 |
Study Result: | Negative |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 83 | 107 | 63 | 25 | 26 |
83 | 98 | 59 | 24 | ||||
81 | 92 | 67 | 28 | ||||
78# | 103 | 62 | 27 | ||||
Test Chemical | D & C red no. 9 | 1.25 | 73 | 67 | 60 | 28 | 34 |
85 | 75 | 100 | 39 | ||||
78 | 83 | 82 | 35 | ||||
2.5 | 91 | 71 | 89 | 33 | 31 | ||
84 | 70 | 76 | 30 | ||||
105 | 79 | 95 | 30 | ||||
5 | 105 | 98 | 97 | 31 | 26 | ||
101 | 82 | 88 | 29 | ||||
98 | 115 | 53 | 18 | ||||
7.5 | 104# | 89 | 88 | 28 | 35 | ||
91 | 78 | 114 | 42 | ||||
93 | 85 | 95 | 34 | ||||
15 | 86# | 77 | 69 | 27 | 26 | ||
86 | 85 | 59 | 23 | ||||
86# | 74 | 70.5 | 27 | ||||
Positive Control | Methyl Methane Sulfonate | 5 | 51 | 40 | 611 | 403 | 361* |
35 | 20 | 164 | 155 | ||||
40# | 30 | 634 | 524 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 96 | 107 | 72 | 25 | 31 |
87 | 91 | 85 | 33 | ||||
93 | 115 | 94 | 34 | ||||
88 | 87 | 88 | 34 | ||||
Test Chemical | D & C red no. 9 | 1.25 | 91 | 83 | 86 | 32 | 32 |
83 | 103 | 84 | 34 | ||||
76 | 83 | 69 | 30 | ||||
2.5 | 96 | 85 | 109 | 38 | 34 | ||
94 | 105 | 84 | 30 | ||||
94 | 94 | 96 | 34 | ||||
5 | 96 | 86 | 119 | 41 | 33 | ||
92 | 90 | 93 | 34 | ||||
99 | 111 | 70 | 24 | ||||
7.5 | 96 | 94 | 137 | 48 | 44 | ||
103 | 90 | 126 | 41 | ||||
109 | 88 | 141 | 43 | ||||
10 | 99 | 71 | 119 | 40 | 44 | ||
99 | 70 | 141 | 47 | ||||
104 | 95 | 139 | 44 | ||||
15 | 121r | 97 | 143 | 40 | 35 | ||
113 | 96 | 112 | 33 | ||||
89 | 88 | 101 | 38 | ||||
Positive Control | Methyl Methane Sulfonate | 5 | 51 | 32 | 399 | 263 | 273* |
78 | 61 | 517 | 221 | ||||
44 | 24 | 439 | 335 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 98 | 101 | 76 | 26 | 27 |
73 | 93 | 74 | 34 | ||||
87 | 105 | 64 | 25 | ||||
75 | 101 | 56 | 25 | ||||
Test Chemical | D & C red no. 9 | 2 | 100 | 82 | 82 | 27 | 26 |
92 | 79 | 82 | 30 | ||||
111 | 102 | 73 | 22 | ||||
3 | 95 | 100 | 66 | 23 | 21 | ||
92 | 94 | 57 | 21 | ||||
88 | 90 | 54 | 20 | ||||
4 | 110 | 117 | 58 | 18 | 16 | ||
99 | 90 | 54 | 18 | ||||
111 | 98 | 39 | 12 | ||||
5 | 111# | 93 | 107 | 32 | 26 | ||
110 | 105 | 77 | 23 | ||||
105 | 97 | 66 | 21 | ||||
6 | 116 | 76 | 72 | 21 | 26 | ||
94 | 111 | 88 | 31 | ||||
105 | 105 | 78 | 25 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 91 | 81 | 448 | 164 | 160* |
72 | 89 | 359 | 166 | ||||
99 | 78 | 450 | 152 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 106 | 111 | 106 | 33 | 34 |
114 | 95 | 94 | 27 | ||||
121r | 90 | 106 | 29 | ||||
107 | 94 | 130 | 41 | ||||
Test Chemical | D & C red no. 9 | 2 | 104 | 61 | 146 | 47 | 40 |
125r | 93 | 117 | 31 | ||||
94 | 76 | 96 | 34 | ||||
3 | 114 | 65 | 152 | 45 | 39 | ||
116 | 80 | 121 | 35 | ||||
112 | 75 | 126 | 37 | ||||
4 | 101 | 74 | 163 | 54 | 48 | ||
102 | 78 | 130 | 42 | ||||
110 | 81 | 154 | 47 | ||||
5 | 111 | 79 | 167 | 50 | 46 | ||
112 | 83 | 156 | 46 | ||||
94 | 83 | 119 | 42 | ||||
6 | 113 | 72 | 164 | 48 | 41 | ||
117 | 76 | 108 | 31 | ||||
93 | 63 | 119 | 43 | ||||
8 | 122r | 94 | 145 | 40 | 41 | ||
113 | 83 | 121 | 36 | ||||
110 | 60 | 154 | 47 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 86# | 22 | 815 | 315 | 393* |
53 | 19 | 622 | 392 | ||||
57 | 17 | 807 | 471 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination