Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01940-0009-0000-5
Chemical Name:	D & C red no. 9
CASRN:	5160-02-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	494246
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	107	63	25	26
			83	98	59	24
			81	92	67	28
			78#	103	62	27
Test Chemical	D & C red no. 9	1.25	73	67	60	28	34
			85	75	100	39
			78	83	82	35
		2.5	91	71	89	33	31
			84	70	76	30
			105	79	95	30
		5	105	98	97	31	26
			101	82	88	29
			98	115	53	18
		7.5	104#	89	88	28	35
			91	78	114	42
			93	85	95	34
		15	86#	77	69	27	26
			86	85	59	23
			86#	74	70.5	27
Positive Control	Methyl Methane Sulfonate	5	51	40	611	403	361*
			35	20	164	155
			40#	30	634	524

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	107	72	25	31
			87	91	85	33
			93	115	94	34
			88	87	88	34
Test Chemical	D & C red no. 9	1.25	91	83	86	32	32
			83	103	84	34
			76	83	69	30
		2.5	96	85	109	38	34
			94	105	84	30
			94	94	96	34
		5	96	86	119	41	33
			92	90	93	34
			99	111	70	24
		7.5	96	94	137	48	44
			103	90	126	41
			109	88	141	43
		10	99	71	119	40	44
			99	70	141	47
			104	95	139	44
		15	121r	97	143	40	35
			113	96	112	33
			89	88	101	38
Positive Control	Methyl Methane Sulfonate	5	51	32	399	263	273*
			78	61	517	221
			44	24	439	335

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	101	76	26	27
			73	93	74	34
			87	105	64	25
			75	101	56	25
Test Chemical	D & C red no. 9	2	100	82	82	27	26
			92	79	82	30
			111	102	73	22
		3	95	100	66	23	21
			92	94	57	21
			88	90	54	20
		4	110	117	58	18	16
			99	90	54	18
			111	98	39	12
		5	111#	93	107	32	26
			110	105	77	23
			105	97	66	21
		6	116	76	72	21	26
			94	111	88	31
			105	105	78	25
Positive Control	3-Methylcholanthrene	2.5	91	81	448	164	160*
			72	89	359	166
			99	78	450	152

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106	111	106	33	34
			114	95	94	27
			121r	90	106	29
			107	94	130	41
Test Chemical	D & C red no. 9	2	104	61	146	47	40
			125r	93	117	31
			94	76	96	34
		3	114	65	152	45	39
			116	80	121	35
			112	75	126	37
		4	101	74	163	54	48
			102	78	130	42
			110	81	154	47
		5	111	79	167	50	46
			112	83	156	46
			94	83	119	42
		6	113	72	164	48	41
			117	76	108	31
			93	63	119	43
		8	122r	94	145	40	41
			113	83	121	36
			110	60	154	47
Positive Control	3-Methylcholanthrene	2.5	86#	22	815	315	393*
			53	19	622	392
			57	17	807	471

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination