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Study Information
Study Attribute Value
CEBS Accession Number: 002-01945-0002-0000-3
Chemical Name: D-Carvone
CASRN: 2244-16-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 983543_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 10/12/1987    Harvest Time: 20.1 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: D-Carvone 
25         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
31.3       
 100 9 0.090 7.0 4 0.040 4.0 5 0.050 3.0 0 0.000 0.0 
37.5       
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 1.0 0 0.000 0.0 
43.8       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 56 0.560 33.0 25 0.250 19.0 31 0.310 21.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 25 1.000 68.0 13 0.520 40.0 12 0.480 40.0 0 0.000 0.0
Trend: 1.021 0.770 0.664  
Probability: 0.154 0.221 0.253  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/12/1987    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: D-Carvone 
250         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
325         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
400         
 100 22 0.220 19.0 2 0.020 2.0 20 0.200 17.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 23 0.920 56.0 12 0.480 40.0 11 0.440 28.0 0 0.000 0.0
Trend: 4.923 1.235 4.769  
Probability: 0.000 0.108 0.000  


Trial #:2   Activation: No Activation    Date: 09/15/1987    Harvest Time: 20.2 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: D-Carvone 
5         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
12.5       
 200 16 0.080 6.5 1 0.005 0.5 15 0.075 6.0 0 0.000 0.0 
25.1       
 200 21 0.105 8.0 0 0.000 0.0 21 0.105 8.0 0 0.000 0.0 
37.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 37 0.185 16.5 22 0.110 10.5 15 0.075 7.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 24 0.960 60.0 11 0.440 28.0 13 0.520 36.0 0 0.000 0.0
Trend: 4.739 0.519 4.716  
Probability: 0.000 0.302 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/15/1987    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 5 0.025 2.0 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: D-Carvone 
75         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 
100         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
250         
 200 13 0.065 5.5 3 0.015 1.5 10 0.050 4.0 0 0.000 0.0 
500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 17 0.085 7.0 7 0.035 3.5 10 0.050 4.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 20 0.800 52.0 9 0.360 28.0 11 0.440 36.0 0 0.000 0.0
Trend: 3.299 1.722 2.796  
Probability: 0.000 0.042 0.003