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Study Information
Study Attribute Value
CEBS Accession Number: 002-01946-0001-0000-3
Chemical Name: D-Limonene
CASRN: 5989-27-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 269844_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Weakly Positive 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 03/19/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 389 0.370 7.78 26.5 0.000
Test Chemical D-Limonene 
16.2       
 50 1050 447 0.426 8.94 26.5 14.910 
54         
 50 1051 463 0.441 9.26 26.5 18.910 
162         
 50 1051 457 0.435 9.14 26.5 17.369
Positive Control Mitomycin-C 
 0.0015    
 50 1048 701 0.669 14.02 26.5 80.550
Mitomycin-C 
 0.01      
 10 211 341 1.616 34.10 26.5 336.226
Trend: 2.342        
Probability: 0.010        

Trial #: 2    Activation: No Activation    Date: 03/26/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 366 0.349 7.32 26.5 0.000
Test Chemical D-Limonene 
30         
 50 1049 407 0.388 8.14 26.5 11.202 
50         
 50 1046 405 0.387 8.10 30.5 10.973 
100         
 50 1041 475 0.456 9.50 30.5 30.779
Positive Control Mitomycin-C 
 0.0015    
 50 1046 476 0.455 9.52 26.5 30.428
Mitomycin-C 
 0.01      
 10 210 252 1.200 25.20 26.5 243.934
Trend: 3.773        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 04/18/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 345 0.329 6.90 26.5 0.000
Test Chemical D-Limonene 
15         
 50 1049 343 0.327 6.86 26.5 -0.674 
30         
 50 1048 349 0.333 6.98 26.5 1.159 
50         
 50 1046 406 0.388 8.12 30.5 17.906
Positive Control Mitomycin-C 
 0.0015    
 50 1051 516 0.491 10.32 26.5 49.138
Mitomycin-C 
 0.01      
 10 209 230 1.100 23.00 26.5 234.290
Trend: 2.175        
Probability: 0.015        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 03/19/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 398 0.380 7.96 26.0 0.000
Test Chemical D-Limonene 
16.2       
 50 1048 404 0.385 8.08 26.0 1.411 
54         
 50 1049 399 0.380 7.98 26.0 0.060 
162         
 50 1045 394 0.377 7.88 26.0 -0.816
Positive Control Cyclophosphamide 
 0.4       
 50 1046 620 0.593 12.40 26.0 55.928
Cyclophosphamide 
2.5       
 10 210 405 1.929 40.50 26.0 407.340
Trend: -0.169        
Probability: 0.567