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Study Information
Study Attribute Value
CEBS Accession Number: 002-01946-0002-0000-4
Chemical Name: D-Limonene
CASRN: 5989-27-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 269844_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/28/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: D-Limonene
10         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
30         
100 5 0.050 2.0 0 0.000 0.0 5 0.050 2.0 0 0.000 0.0
100         
100 6 0.060 6.0 0 0.000 0.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C
5         
50 52 1.040 50.0 14 0.280 18.0 28 0.560 42.0 10 0.200 1.0
Trend: 0.750 0.000 0.750  
Probability: 0.227 0.000 0.227  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/28/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 7 0.070 4.0 1 0.010 1.0 6 0.060 3.0 0 0.000 0.0
Test Chemical: D-Limonene
50         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
150         
100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
500         
100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide
50         
50 26 0.520 40.0 10 0.200 18.0 16 0.320 26.0 0 0.000 0.0
Trend: 0.878 -1.339 1.305  
Probability: 0.190 0.910 0.096