COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01946-0002-0000-4
Chemical Name: D-Limonene
CASRN: 5989-27-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 269844_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/28/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: D-Limonene 
10         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
30         
 100 5 0.050 2.0 0 0.000 0.0 5 0.050 2.0 0 0.000 0.0 
100         
 100 6 0.060 6.0 0 0.000 0.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
5         
 50 52 1.040 50.0 14 0.280 18.0 28 0.560 42.0 10 0.200 1.0
Trend: 0.750 0.000 0.750  
Probability: 0.227 0.000 0.227  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/28/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 7 0.070 4.0 1 0.010 1.0 6 0.060 3.0 0 0.000 0.0
Test Chemical: D-Limonene 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
150         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
500         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 26 0.520 40.0 10 0.200 18.0 16 0.320 26.0 0 0.000 0.0
Trend: 0.878 -1.339 1.305  
Probability: 0.190 0.910 0.096