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Study Information
Study Attribute Value
CEBS Accession Number: 002-01948-0013-0000-8
Chemical Name: D-Mannitol
CASRN: 69-65-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 851315
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 100 111 99 33 40
    93# 77 112 40
    92# 104 136 49
    96 108 111 39
Test Chemical D-Mannitol 
50         
 80 86 58 24 23
    109 129 75 23
    93 115 65 23
  
100         
 103 94 65 21 26
    95 159 88 31
    92 111 68 25
  
200         
 115 103 88 26 27
    87# 71 84 32
    94# 92 65 23
  
300         
 108 117 96 30 31
    121r 96 134 37
    91 86 89 33
  
400         
 114 92 97 28 28
    98# 97 91 31
    114 113 88 26
  
500         
 96# 105 117 41 35
    131r 123 84 21
    97 123 86 30
Positive Control Methyl Methane Sulfonate 
5         
 45# 29 639 475 458*
    60 34 742 413
    44 25 646 486

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109# 93 97 30 28
    93 83 78 28
    122r 114 71 19
    117 124 91 26
Test Chemical D-Mannitol 
125         
 88# 90 47 18 27
    78# 86 84 36
    83 97 66 26
  
250         
 87 94 54 21 25
    79 84 56 24
    85 108 80 31
  
500         
 94 121 82 29 27
    105 121 64 20
    77 66 76 33
  
750         
 108 98 83 26 25
    108 137 81 25
    109 115 81 25
  
1000         
 78 66 72 31 29
    91 108 60 22
    110 95 118 36
  
1500         
 109 112 85 26 27
    93 92 78 28
    103 80 82 27
Positive Control Methyl Methane Sulfonate 
5         
 73# 46 453 206 296*
    66 27 658 335
    77 25 803 347

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 93 101 64 23 29
    91 98 84 31
    87 92 71 27
    108 109 108 33
Test Chemical D-Mannitol 
500         
 86 75 56 22 23
    100 84 56 19
    69 75 62 30
  
1000         
 86 90 50 19 22
    89 73 48 18
    66 58 54 27
  
2000         
 81 71 58 24 21
    90 86 64 24
    64# 64 29 15
  
3000         
 69 66 50 24 20
    81 79 49 20
    99 80 43 14
  
4000         
 91 66 87 32 28
    86 66 58 23
    90 94 77 28
  
5000         
 102 96 56 18 28
    86 78 73 28
    85 79 94 37
Positive Control Methyl Methane Sulfonate 
5         
 95 72 467 164 164*
    84 59 410 164
    90 62 446 165

Nonactivation Trial: 4 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 117 89 143 41 40
    101 78 146 48
    92 103 102 37
    114 131 121 35
Test Chemical D-Mannitol 
500         
 104 125 103 33 38
    110 92 120 36
    99 95 130 44
  
1000         
 94 107 107 38 36
    102 112 99 33
    109 82 121 37
  
2000         
 117# 119 130 37 41
    94 94 125 44
    122r 129 142 39
  
3000         
 103 109 133 43 38
    106 111 108 34
    101 81 113 37
  
4000         
 105 101 139 44 38
    114 123 139 41
    110 131 92 28
  
5000         
 96# 101 133 46 44
    111 121 158 48
    116 107 127 36
Positive Control Methyl Methane Sulfonate 
5         
 102 53 861 283 261*
    98 101 731 250
    112# 92 840 250

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 60 52 17 9 15
    58 85 34 20
    61 142 36 20
    62 121 19 10
Test Chemical D-Mannitol 
500         
 61 111 83 45 59*
    62 111 68 37
    22 52 61 94
  
1000         
 82 173 76 31 38*
    70 127 99 47
    56 104 60 36
  
2000         
 36 47 51 47 75*
    32 49 101 105
    24 48 53 73
  
3000         
 49 65 52 35 32*
    54 92 52 32
    41 67 34 28
  
4000         
 58# 86 35 20 25*
    60 123 44 24
    72 74 69 32
  
5000         
 94 166 19 7 13
    48 67 14 10
    52 85 34 22
Positive Control 3-Methylcholanthrene 
2.5       
 61 37 373 204 174*
    60 40 258 144

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 90 83 77 29 26
    118 118 77 22
    111 115 88 26
    74 84 64 29
Test Chemical D-Mannitol 
500         
 86 90 95 37 36
    108 104 101 31
    87 73 101 39
  
1000         
 96 101 80 28 28
    75 74 64 28
    94 82 79 28
  
2000         
 84 94 71 28 25
    94 85 66 23
    103 99 69 22
  
3000         
 108 101 91 28 25
    129r 83 156 40
    105 100 68 22
  
4000         
 79 80 112 47 40*
    89 105 108 41
    100 100 99 33
  
5000         
 97 100 83 28 36
    88 90 83 31
    90 63 127 47
Positive Control 3-Methylcholanthrene 
2.5       
 74 48 652 292 210*
    106 85 406 128
    123r 97 398 108

Induced S9 Trial: 3 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 67 90 169 85 78
    81 96 180 74
    80 99 168 70
    80 115 200 83
Test Chemical D-Mannitol 
500         
 84 123 177 70 72
    80 135 172 72
    76 106 171 75
  
1000         
 61# 108 86 47 59
    91 115 169 62
    82 104 171 69
  
2000         
 78 92 133 57 57
    93 107 140 50
    87 95 163 62
  
3000         
 96 132 153 53 55
    77 109 121 53
    72 111 126 59
  
4000         
 97 100 160 55 58
    105 105 164 52
    81 89 164 67
  
5000         
 91 91 171 63 93
    113 114 468 138
    73 87 172 79
Positive Control 3-Methylcholanthrene 
2.5       
 68 69 494 242 208*
    59 80 390 220
    56 88 272 161

Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 118 103 115 32 33
    104 115 124 40
    103 79 102 33
    109 103 91 28
Test Chemical D-Mannitol 
500         
 97 118 117 40 40
    114 101 123 36
    109 94 143 44
  
1000         
 118 85 137 39 41
    104 80 121 39
    99 95 133 45
  
2000         
 107 90 146 46 42
    101 75 148 49
    103 95 100 32
  
3000         
 104 102 100 32 42
    123r 93 111 30
    94 80 149 53
  
4000         
 116 94 98 28 31
    108 87 109 34
    103 92 97 31
  
5000         
 126r 121 85 23
    124r 90 127 34
    140r 104 142 34
Positive Control Methyl Methane Sulfonate 
5         
 74 31 901 409 345*
    84 50 708 280
    62 29 644 347

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination