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Study Information
Study Attribute Value
CEBS Accession Number: 002-01950-0002-0000-9
Chemical Name: D-alpha-Tocopheryl succinate
CASRN: 4345-03-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 185103_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Test Failure 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 
Induced Rat Liver S9  3_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/01/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: D-alpha-Tocopheryl succinate 
39.8       
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
49.8       
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
60         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
75         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 100 16 0.160 11.0 5 0.050 5.0 9 0.090 8.0 2 0.020 1.0
Mitomycin-C 
 0.5       
 25 7 0.280 28.0 5 0.200 20.0 2 0.080 8.0 0 0.000 0.0
Trend: 1.337 1.804 -0.453  
Probability: 0.091 0.036 0.675  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/01/1984    Harvest Time: 10.5 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 5 0.050 4.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: D-alpha-Tocopheryl succinate 
1000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
37.5       
 25 16 0.640 40.0 3 0.120 12.0 13 0.520 36.0 0 0.000 0.0
Trend: 0.000 0.000 0.000  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 01/26/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 5 0.050 5.0 2 0.020 2.0 2 0.020 2.0 1 0.010 1.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: D-alpha-Tocopheryl succinate 
24.9       
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
30.1       
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0 
35         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 19 0.190 15.0 8 0.080 7.0 11 0.110 8.0 0 0.000 0.0
Mitomycin-C 
 0.5       
 50 17 0.340 24.0 7 0.140 12.0 10 0.200 18.0 0 0.000 0.0
Trend: 0.251 -1.070 1.148  
Probability: 0.401 0.858 0.125  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/11/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 2.0 0 0.000 0.0 3 0.030 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 2.0 0 0.000 0.0 3 0.030 2.0 0 0.000 0.0
Test Chemical: D-alpha-Tocopheryl succinate 
400         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
450         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
500         
 100 7 0.070 3.0 0 0.000 0.0 7 0.070 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 50 16 0.320 22.0 6 0.120 10.0 10 0.200 16.0 0 0.000 0.0
Cyclophosphamide 
50         
 100 12 0.120 12.0 5 0.050 5.0 7 0.070 7.0 0 0.000 0.0
Trend: 0.277 0.000 0.277  
Probability: 0.391 0.000 0.391  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 03/22/1984    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: D-alpha-Tocopheryl succinate 
4100         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
6000         
 100 2 0.020 1.0 0 0.000 0.0 2 0.020 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
5         
 100 8 0.080 8.0 0 0.000 0.0 8 0.080 8.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 9 0.360 28.0 3 0.120 8.0 5 0.200 20.0 1 0.040 4.0
Trend: -0.188 0.474 -0.448  
Probability: 0.574 0.318 0.673