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Study Information
Study Attribute Value
CEBS Accession Number: 002-01957-0001-0000-5
Chemical Name: DL-menthol
CASRN: 89-78-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 423321_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Questionable 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/17/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1020 386 0.378 7.72 26.0 0.000
Test Chemical DL-menthol 
5         
 50 1029 467 0.454 9.34 26.0 19.926 
16.7       
 50 1022 403 0.394 8.06 26.0 4.200 
50         
 50 1023 397 0.388 7.94 26.0 2.548 
167         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1017 649 0.638 12.98 26.0 68.631
Mitomycin-C 
 0.01      
 5 100 178 1.780 35.60 26.0 370.363
Trend: -0.348        
Probability: 0.636        

Trial #: 2    Activation: No Activation    Date: 08/07/1985    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1022 418 0.409 8.36 26.0 0.000
Test Chemical DL-menthol 
2.5       
 50 1016 389 0.383 7.78 26.0 -6.388 
5         
 50 1025 451 0.440 9.02 26.0 7.579 
10         
 50 1000 468 0.468 9.36 26.0 14.425 
25         
 50 1026 502 0.489 10.04 26.0 19.627
Positive Control Mitomycin-C 
 0.001     
 50 1027 544 0.530 10.88 26.0 29.510
Mitomycin-C 
 0.01      
 5 105 158 1.505 31.60 26.0 267.911
Trend: 3.722        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/17/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1011 421 0.416 8.42 26.0 0.000
Test Chemical DL-menthol 
16.7       
 50 1020 405 0.397 8.10 26.0 -4.649 
50         
 50 1004 437 0.435 8.74 26.0 4.524 
167         
 50 1028 423 0.411 8.46 26.0 -1.186 
500         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1035 663 0.641 13.26 26.0 53.830
Cyclophosphamide 
2         
 5 97 198 2.041 39.60 26.0 390.188
Trend: 0.237        
Probability: 0.406