Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01960-0009-0000-7
Chemical Name:	Daminozide
CASRN:	1596-84-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	001610
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Solvent	0	95	100	50	18	17
			49r	53	28	19
			93	104	62	22
			91	96	30	11
Test Chemical	Daminozide	156.25	107	74	90	28	32*
			55	42	60	36	32*
		312.5	46	35	42	30	32*
			53	62	52	33	32*
		625	33	36	73	73
		1250	56	53	77	46	43*
			44	51	54	41	43*
		2500

Positive Control	Other Positive Control	15	18	6	110	204	200*
Positive Control			23	12	133	196	200*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	71#	105	135	64	68
			90#	107	171	63
			81	87	186	77
Test Chemical	Daminozide	1000	76#	82	190.5	84
		1200	80#	77	190.5	80	75
			69	69	146	71	75
		1400	72#	67	199	92	79
			69#	77	135	65	79
		1600	74#	77	144	65	76
			66#	72	173	88	76
		1800	74	41	281	126	172*
			58#	14	381	218	172*
		2000

Positive Control	Methyl Methane Sulfonate	15	32#	22	508	533	440*
Positive Control			46#	25	476	347	440*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	99	102	143	48	50
			94	103	129	46
			124r	106	172	46
			96	95	162	56
Test Chemical	Daminozide	1500	101#	80	199.5	66	54
			100	71	125	42	54
		1600	107	80	160	50	72
			68#	50	192	94	72
		1700	98	72	123	42	51
			107	63	192	60	51
		1800	94	68	133	47	60
			86	44	185	72	60
		1900	45	3	220	165
			124r	19	217	58
		2000

Positive Control	Methyl Methane Sulfonate	15	61	41	372	202	188*
Positive Control			72	53	374	173	188*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	84	99	75	30	33
			102	116	104	34
			89	96	106	40
			73	89	64	29
Test Chemical	Daminozide	1500	88	72	113	43	46
			93	70	134	48	46
		1600	81	61	77	32	30
			85	85	70	27	30
		1700	87	69	61	23	34
			91	65	122	45	34
		1800	101	49	126	42	45
			94	37	138	49	45
Positive Control	Methyl Methane Sulfonate	15	43	29	219	168	142*
Positive Control			49	26	169	116	142*

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	85	111	134	53	57
			94	100	145	51
			76	98	147	64
			80	91	139	58
Test Chemical	Daminozide	1320	101	101	116	38	46
			91	123	144	53	46
		1400	86	116	117	45	46
			85	112	119	47	46
		1480	77	101	140	61	54
			93	111	134	48	54
		1560	89	116	158	59	54
			80	101	117	49	54
		1640	79	126	122	52	55
			94	121	165	59	55
Positive Control	3-Methylcholanthrene	2.5	48	68	333	232	215*
Positive Control			56	96	334	199	215*

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	90	108	70	26	22
			88	90	42	16
			84	113	46	18
			73	89	62	28
Test Chemical	Daminozide	1550	96	92	81	28	33
			85	93	95	37	33
		1650	82	85	101	41	38*
			80	85	85	35	38*
		1750	80	80	77	32	36
			69	83	81	39	36
		1850	92	68	96	35	30
			93	90	73	26	30
		1950	99	65	133	45	37*
			78	61	69	30	37*
Positive Control	3-Methylcholanthrene	2.5	47	22	181	128	135*
Positive Control			42	12	178	141	135*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	94	99	266	94	76
			94	100	180	64
			90	112	185	69
			92	89	210	77
Test Chemical	Daminozide	1550	108	88	283	88	86
			100	111	254	85	86
		1650	104	95	252	81	83
			111	92	281	85	83
		1750	94	103	240	86	89
			105	80	290	92	89
		1850	103	80	405	131	107
			104	66	260	83	107
		1950	85	31	325	128	125*
			74	36	269	122	125*
		2050

Positive Control	3-Methylcholanthrene	2.5	91	58	957	349	322*
Positive Control			100	60	878	294	322*

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	83	97	99	40	32
			62	92	53	29
			102	116	78	26
			73	96	73	33
Test Chemical	Daminozide	1650	78	99	70	30	31
			81	97	79	33	31
		1750	74	86	91	41	37
			94	84	91	32	37
		1850	80	90	102	43	42
			75	83	91	41	42
		1950	95	79	129	45	42
			100	76	115	38	42
		2150

Positive Control	3-Methylcholanthrene	2.5	67	79	275	137	125*
Positive Control			71	80	242	114	125*

Induced S9 Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	95	102	65	23	23
			92	113	43	16
			74	90	65	29
			96	95	67	23
Test Chemical	Daminozide	1550	155r	91	109	23
			104	90	83	27
		1650	93	95	119	43	40*
			90	91	102	38	40*
		1750	94	85	112	40	38*
			86	80	91	35	38*
		1850	101	64	127	42	44*
			80	69	112	47	44*
		1950	82	24	174	71	101*
			66	10	259	131	101*
		2050

Positive Control	3-Methylcholanthrene	2.5	55	32	507	306	296*
Positive Control			74	34	632	285	296*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination