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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0010-0000-8
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 923560_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 12/08/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1038 347 0.334 6.94 26.0 -9.312
Dimethyl Sulfoxide 
0         
 50 1039 383 0.369 7.66 26.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
5         
 50 1045 394 0.377 7.88 26.0 2.281 
16         
 50 1034 385 0.372 7.70 26.0 1.008 
50         
 50 1036 432 0.417 8.64 34.0 13.120 
160         
 50 1036 453 0.437 9.06 34.0 18.619 
500         
 50 1037 461 0.445 9.22 34.0 20.598 
1600         
 0 0 0 0.000 0.00 34.0 N/A 
5000         
 0 0 0 0.000 0.00 34.0 N/A
Positive Control Mitomycin-C 
 0.01      
 50 1035 2265 2.188 45.30 26.0 493.669
Trend: 3.669        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/21/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 404 0.387 8.08 26.0 0.000
Negative (Not Specified) 
0         
 50 1040 405 0.389 8.10 26.0 0.729
Test Chemical Di(2-ethylhexyl) phthalate 
2000         
 50 1032 463 0.449 9.26 40.0 16.048 
3000         
 50 1043 507 0.486 10.14 40.0 25.736 
4000         
 50 1034 535 0.517 10.70 40.0 33.835 
5000         
 50 1043 518 0.497 10.36 40.0 28.464
Positive Control Mitomycin-C 
 0.01      
 50 1050 2464 2.347 49.28 26.0 506.997
Trend: 4.468        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 02/28/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1034 405 0.392 8.10 26.0 3.851
Dimethyl Sulfoxide 
0         
 50 1042 393 0.377 7.86 26.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
3000         
 50 1024 460 0.449 9.20 40.0 19.106 
4000         
 50 1047 481 0.459 9.62 40.0 21.807 
5000         
 50 1034 525 0.508 10.50 40.0 34.621
Positive Control Mitomycin-C 
 0.005     
 50 1044 1785 1.710 35.70 26.0 353.328
Mitomycin-C 
 0.005     
 0 0 0 0.000 0.00 40.0 N/A
Trend: 4.299        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/08/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 362 0.349 7.24 26.0 0.000
Negative (Not Specified) 
0         
 50 1034 374 0.362 7.48 26.0 3.515
Test Chemical Di(2-ethylhexyl) phthalate 
160         
 50 1042 408 0.392 8.16 26.0 12.058 
500         
 50 1038 388 0.374 7.76 26.0 6.976 
1600         
 50 1029 410 0.398 8.20 26.0 14.030 
5000         
 50 1035 398 0.385 7.96 26.0 10.051
Positive Control Cyclophosphamide 
1.5       
 50 1033 1301 1.259 26.02 26.0 260.436
Trend: 1.273        
Probability: 0.101        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/21/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1035 358 0.346 7.16 26.0 0.000
Negative (Not Specified) 
0         
 50 1030 381 0.370 7.62 26.0 6.941
Test Chemical Di(2-ethylhexyl) phthalate 
3000         
 50 1036 412 0.398 8.24 26.0 14.973 
4000         
 50 1039 410 0.395 8.20 26.0 14.084 
5000         
 50 1037 424 0.409 8.48 26.0 18.207
Positive Control Cyclophosphamide 
2         
 50 1037 1833 1.768 36.66 26.0 411.024
Trend: 2.144        
Probability: 0.016