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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0012-0000-0
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 978423_SCE
Study Result: Equivocal

Study Result: Equivocal
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/24/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 400 0.383 8.00 27.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
 0.5       
 50 1050 468 0.446 9.36 27.0 16.443 
1.6       
 50 1042 405 0.389 8.10 27.0 1.542 
5         
 50 1044 434 0.416 8.68 27.0 8.604 
16         
 0 0 0 0.000 0.00 27.0 N/A 
50         
 0 0 0 0.000 0.00 27.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 588 0.560 11.76 27.0 46.300
Mitomycin-C 
 0.01      
 10 210 467 2.224 46.70 27.0 480.970
Trend: 0.466        
Probability: 0.321        

Trial #: 2    Activation: No Activation    Date: 08/30/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 475 0.454 9.50 25.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
 0.5       
 50 1048 467 0.446 9.34 27.0 -1.778 
1         
 50 1047 440 0.420 8.80 27.0 -7.368 
2.5       
 50 1049 543 0.518 10.86 27.0 14.098 
5         
 50 1049 482 0.459 9.64 27.0 1.280 
10         
 50 1050 484 0.461 9.68 27.0 1.604 
20         
 50 1050 497 0.473 9.94 35.5 4.333
Positive Control Mitomycin-C 
 0.0E-4    
 50 1044 581 0.557 11.62 25.0 22.667
Mitomycin-C 
 0.01      
 10 210 387 1.843 38.70 25.0 306.205
Trend: 1.257        
Probability: 0.104        

Trial #: 3    Activation: No Activation    Date: 08/30/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 475 0.454 9.50 25.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
30         
 50 1044 539 0.516 10.78 48.5 13.800 
30         
 0 0 0 0.000 0.00 35.5 N/A 
40         
 50 1042 536 0.514 10.72 48.5 13.384 
50         
 50 1047 606 0.579 12.12 53.5 27.579 
100         
 50 1048 623 0.594 12.46 53.5 31.033
Positive Control Mitomycin-C 
 0.0E-4    
 50 1044 581 0.557 11.62 25.0 22.667
Mitomycin-C 
 0.01      
 10 210 387 1.843 38.70 25.0 306.205
Trend: 4.602        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/26/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 455 0.433 9.10 26.0 0.000
Test Chemical Di(2-ethylhexyl) phthalate 
160         
 50 1049 402 0.383 8.04 26.0 -11.480 
500         
 50 1049 437 0.417 8.74 26.0 -3.773 
1600         
 50 1049 430 0.410 8.60 26.0 -5.314 
2500         
 50 1047 443 0.423 8.86 26.0 -2.265
Positive Control Cyclophosphamide 
 0.3       
 50 1048 644 0.615 12.88 26.0 41.944
Cyclophosphamide 
2         
 10 210 415 1.976 41.50 26.0 356.478
Trend: 0.092        
Probability: 0.463