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Study Information
Study Attribute Value
CEBS Accession Number: 002-01969-0035-0000-5
Chemical Name: Di(2-ethylhexyl) phthalate
CASRN: 117-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 913224
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 44r 88 101 77 60
    53 95 113 71
    53 89 88 55
    76# 116 121 53
Test Chemical Di(2-ethylhexyl) phthalate 
 0.25      
 82 115 85 35 35
    88 93 114 43
    86 124 70 27
  
 0.5       
 78 95 94 40 41
    57 75 86 50
    100# 54 98 33
  
1         
 66 58 87 44 49
    60# 39 99 55
  
2         
 65r 41 82 42
  
3         
 56 21 137 82 71
    57 29 103 61
  
5         
 42 9 100 80 80
    50 20 103 68
    42 18 114 92
Positive Control Methyl Methane Sulfonate 
10         
 34 19 622 616 634*
    31 32 627 667
    34 30 626 620

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 106 88 96 30 30
    91 104 78 29
    107 108 96 30
Test Chemical Di(2-ethylhexyl) phthalate 
 0.125     
 66 22 100 50 48*
    72 27 99 46
  
 0.25      
 61 16 84 46 44
    68 18 79 39
    63 15 91 48
  
 0.5       
 60 24 90 50 44
    66# 19 76 38
    79 26 105 44
  
1         
 78 16 111 47 55*
    60# 6 109 61
    73 17 122 56
  
3         
 47 11 57 41 41
    61 13 88 48
    75 13 74 33
  
5         
 54 19 57 35 39
    60 12 71 40
    63 10 77 41
Positive Control Methyl Methane Sulfonate 
5         
 70 54 519 248 248*
Methyl Methane Sulfonate 
10         
 52# 25 664 428 436*
    43# 23 573 444

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 88 88 78 30 34
    102 105 106 35
    98 121 97 33
    71 87 78 37
Test Chemical Di(2-ethylhexyl) phthalate 
1.25      
 76 93 85 37 37
    68 77 84 41
    66 77 65 33
  
2.5       
 69 81 96 46 39
    66 72 82 41
    70 84 63 30
  
5         
 85 70 100 39 37
    75 76 78 35
    98 75 106 36
  
10         
 91 85 83 30 37
    77 61 93 40
    82 54 99 40
  
20         
 87 58 86 33 41
    67 57 88 44
    61 51 87 47
  
40         
 70 20 115 55 50*
    50 27 76 51
    64 26 85 44
Positive Control Methyl Methane Sulfonate 
5         
 50 32 666 447 390*
    54 34 560 349
    50 33 564 375

Non-Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 108 143 41 34
    121r 95 152 42
    99 103 63 21
    99 90 116 39
Test Chemical Di(2-ethylhexyl) phthalate 
 0.5       
 76 27 97 43 46
    72# 37 90 42
    60# 28 96 53
  
 0.75      
 94 42 94 33 41
    74 41 111 50
    84# 33 103 41
  
1         
 79# 23 78 33 39
    106 34 158 50
    115# 52 114 33
  
1.5       
 108# 29 149 46 42
    102 33 119 39
    110 39 137 42
  
2         
 65# 17 106 54 35
    108 37 85 26
    92 41 66 24
  
3         
 88 33 116 44 40
    100 32 120 40
    71 27 77 36
Positive Control 3-Methylcholanthrene 
3.5       
 116 97 417 119 136*
    99 86 407 138
    89 86 400 151

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 95 99 124 43 37
    89# 99 86 32
    108 110 114 35
    70 92 79 37
Test Chemical Di(2-ethylhexyl) phthalate 
 0.125     
 58 38 134 77 74*
    32# 20 106 110
    85# 43 91 36
  
 0.25      
 54 56 85 52 56*
    51 57 101 66
    61# 63 88 48
  
 0.5       
 78 42 112 48 49
    58 40 89 51
    88 49 130 49
  
1         
 71 48 120 56 51
    70 28 104 49
    65 37 90 46
  
3         
 43# 22 82 64 52
    55 33 75 46
    53 35 73 46
  
5         
 33 16 94 95 74*
    49 17 100 68
    65 22 113 58
Positive Control 3-Methylcholanthrene 
2.5       
 87# 72 429 165 194*
    98 67 653 223

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 72 112 93 43 51
    84 101 140 56
    72 92 116 54
    87 95 137 53
Test Chemical Di(2-ethylhexyl) phthalate 
 0.25      
 91 112 115 42 49
    64 55 112 58
    53# 62 76 48
  
 0.5       
 69# 60 89 43 46
    64 67 77 40
    74 80 125 56
  
 0.75      
 53 56 71 45 46
    79# 63 112 47
  
1         
 73 73 142 65 51
    74 62 87 39
    53 50 76 48
  
2         
 80 67 142 59 51
    96 49 128 44
    82 87 118 48
  
3         
 68 52 124 60 47
    100 73 97 32
    69 65 98 47
Positive Control 3-Methylcholanthrene 
2.5       
 66 49 557 281 270*
    72 60 563 261
    83 67 666 268

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 87 98 160 61 64
    84 104 177 70
    71# 92 131 62
    104 106 194 62
Test Chemical Di(2-ethylhexyl) phthalate 
1.25      
 71 77 128 60 67
    82 88 191 78
    86 77 161 62
  
2.5       
 86 97 143 56 61
    83 108 136 55
    92 93 203 73
  
5         
 83 90 141 56 50
    90 91 125 46
    103 106 143 46
  
10         
 98 90 202 68 64
    95 99 166 58
    75 95 149 66
  
20         
 71 84 150 70 65
    87 107 140 54
    75 69 157 70
  
40         
 101 94 184 61 73
    92 92 209 75
    82 83 203 82
Positive Control 3-Methylcholanthrene 
2.5       
 69 33 775 377 495*
    37 18 729 654
    52 27 707 455

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination