Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01970-0012-0000-2
Chemical Name:	Di(2-ethylhexyl)adipate
CASRN:	103-23-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	190909
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	85	114	67	26	24
			84#	85	49	19
			78	96	60	26
			91#	105	65	24
Test Chemical	Di(2-ethylhexyl)adipate	312.5	80#	80	57	24	26
			75	74	64	28	26
		625	80	70	64	27	20
			88	75	35	13	20
		1250	75	38	39	17	23
			69	25	58	28	23
		2500	70	16	68	32	27
			79	13	50	21	27
Positive Control	Methyl Methane Sulfonate	15	44#	24	156	118	118*
Positive Control			32	25	112	119	118*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	84	98	63	25	22
			103	104	61	20
			63	97	31	16
			92	100	75	27
Test Chemical	Di(2-ethylhexyl)adipate	1800	88	26	41	16	18
			74	25	47	21	18
		2600	73	24	51	23	18
			88	23	36	14	18
		3400	97	20	48	17	16
			93	15	44	16	16
		4200	78	15	35	15	20
			69	13	50	24	20
		5000	72	13	36	17	17
			74	12	37	17	17
Positive Control	Methyl Methane Sulfonate	15	39	34	97	84	83*
Positive Control			44	29	110	83	83*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	73	94	78	36	33
			70	98	88	42
			76	109	55	24
			80	98	72	30
Test Chemical	Di(2-ethylhexyl)adipate	1000	75	38	90	40	32
			65	39	47	24	32
		2000	48	22	43	30	33
			50	27	53	36	33
		3000	59	19	65	37	34
			59	18	55	31	34
		4000	53	22	66	41	39
			56	16	61	37	39
		5000	48	13	53	37	34
			40	16	37	31	34
Positive Control	3-Methylcholanthrene	2.5	32	21	223	231	222*
Positive Control			29	16	187	214	222*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	68	82	102	50	37
			100	101	111	37
			109	101	114	35
			102	116	82	27
Test Chemical	Di(2-ethylhexyl)adipate	1000	100	67	115	38	40
			93	70	116	42	40
		2000	111	28	294	88	73*
			101	32	176	58	73*
		3000	91	21	242	89	85*
			93	23	226	81	85*
		4000	100	26	238	79	80*
			78	20	187	80	80*
		5000	77	27	177	77	81*
			94	28	240	85	81*
Positive Control	3-Methylcholanthrene	2.5	55	11	592	361	363*
Positive Control			55	11	603	364	363*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	64	82	85	45	28
			89	113	56	21
			90	93	66	25
			103	112	63	20
Test Chemical	Di(2-ethylhexyl)adipate	1000	75	26	69	31	34
			61	29	66	36	34
		2000	65	19	38	20	20
			74	20	47	21	20
		3000	72	22	52	24	27
			66	21	60	30	27
		4000	62	20	56	30	35
			47	9	57	41	35
		5000	90	23	81	30	26
			69	22	46	22	26
Positive Control	3-Methylcholanthrene	2.5	50	35	418	281	275*
Positive Control			52	41	420	269	275*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination