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Study Information
Study Attribute Value
CEBS Accession Number: 002-01984-0012-0000-7
Chemical Name: Diallyl phthalate
CASRN: 131-17-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 871217
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 61 94 78 43 51
    47r 74 81 57
    73 111 82 38
    72 94 154 71
Test Chemical Diallyl phthalate 
30         
 69 98 62 30 30
    73 108 71 32
    62 89 54 29
  
40         
 95 120 67 24 25
    85 101 73 29
    96 120 70 24
  
50         
 68 74 79 39 38
    79 106 83 35
    57 89 68 40
  
60         
 61 76 67 36 38
    84 78 118 47
    50# 70 44 30
  
80         
 76 53 88 39 33
    76 72 60 26
    84 68 84 33
Positive Control Methyl Methane Sulfonate 
5         
 51 63 508 332 366*
    44# 43 590 444
    54 54 525 323

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 99 104 94 32 31
    87 83 84 32
    95 100 86 30
    96 113 81 28
Test Chemical Diallyl phthalate 
30         
 91 114 67 24 29
    83 115 73 29
    83 85 78 32
  
40         
 73 72 60 27 28
    94 137 67 24
    77 81 76 33
  
50         
 84 72 68 27 28
    88 98 84 32
    81 69 62 25
  
60         
 89 87 76 29 30
    79 75 79 34
    81 99 69 28
  
80         
 103 102 80 26 28
    95 71 88 31
    100 100 82 27
  
100         
 85 62 73 29 29
    92 56 84 31
    97 59 80 27
  
120         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 66 60 652 331 324*
    70 62 609 289
    57 60 600 351

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 85 57 38 15 23
    76 112 63 28
    93 140 67 24
    92 91 68 25
Test Chemical Diallyl phthalate 
60         
 67 23 131 65 45*
    107 31 100 31
    72 29 86 40
  
80         
 60 9 237 132 110*
    76 11 222 98
    55 10 166 101
  
90         
 102*
    76 10 282 124
    76 11 183 81
  
100         
 125*
    80 7 274 115
    44 5 178 135
  
110         
   
  
120         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 75 70 372 165 166*
    78 63 355 152
    65 62 359 183

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 76 92 132 58 52
    85 104 114 45
    82 111 111 45
    79 93 141 60
Test Chemical Diallyl phthalate 
12.5       
 30 24 189 210 120*
    79 68 188 80
    66 62 139 70
  
25         
 85 77 181 71 67
    69 76 143 69
    87 68 157 60
  
50         
 75 51 166 74 80*
    58 39 152 88
    95 56 220 78
  
75         
 83 51 247 100 109*
    61 46 212 116
    69 62 229 111
  
100         
 77 37 327 141 126*
    85 42 302 119
    95 44 335 118
  
150         
 42 13 455 360 323*
    59 12 577 328
    60 15 508 281
Positive Control 3-Methylcholanthrene 
2.5       
 47 34 712 505 557*
    34 29 562 551
    33 21 601 616

Induced S9 Trial: 2 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 69 12 85 41 30
    111 141 56 17
    110 109 99 30
    87 138 85 33
Test Chemical Diallyl phthalate 
25         
 94 69 87 31 36
    89 67 111 41
    83 60 88 35
  
50         
 88 39 57 22 19
    84 36 33 13
    64 30 45 24
  
75         
 57 17 79 46 42
    58 19 76 43
    52 13 55 36
  
100         
 56 10 78 47 58*
    48 7 79 55
    29 4 62 72
  
150         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 41 18 278 226 233*
    63 30 337 177
    63 29 559 294

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 40r 68 187 155 90
    44r 96 230 174
    58 105 188 107
    64 95 138 72
Test Chemical Diallyl phthalate 
12.5       
 81 124 119 49 64
    58 98 119 68
    50 99 113 75
  
25         
 60 119 180 99 100
    66 95 222 112
    65 74 169 87
  
50         
 51# 69 171 113 124
    47 60 192 135
    49 53 185 125
  
75         
 56 53 253 151 173*
    49 49 224 152
    48 36 313 216
  
100         
 53 35 388 244 204*
    53 30 368 233
    52 36 212 136
  
150         
 28 9 440 530 789*
    19 3 587 1048
    9r 2 423 1659
  
200         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 1156*
    29 17 762 881
    17 8 737 1431

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination