Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01984-0012-0000-7
Chemical Name:	Diallyl phthalate
CASRN:	131-17-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	871217
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	61	94	78	43	51
			47r	74	81	57
			73	111	82	38
			72	94	154	71
Test Chemical	Diallyl phthalate	30	69	98	62	30	30
			73	108	71	32
			62	89	54	29
		40	95	120	67	24	25
			85	101	73	29
			96	120	70	24
		50	68	74	79	39	38
			79	106	83	35
			57	89	68	40
		60	61	76	67	36	38
			84	78	118	47
			50#	70	44	30
		80	76	53	88	39	33
			76	72	60	26
			84	68	84	33
Positive Control	Methyl Methane Sulfonate	5	51	63	508	332	366*
			44#	43	590	444
			54	54	525	323

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	99	104	94	32	31
			87	83	84	32
			95	100	86	30
			96	113	81	28
Test Chemical	Diallyl phthalate	30	91	114	67	24	29
			83	115	73	29
			83	85	78	32
		40	73	72	60	27	28
			94	137	67	24
			77	81	76	33
		50	84	72	68	27	28
			88	98	84	32
			81	69	62	25
		60	89	87	76	29	30
			79	75	79	34
			81	99	69	28
		80	103	102	80	26	28
			95	71	88	31
			100	100	82	27
		100	85	62	73	29	29
			92	56	84	31
			97	59	80	27
		120


Positive Control	Methyl Methane Sulfonate	5	66	60	652	331	324*
			70	62	609	289
			57	60	600	351

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	85	57	38	15	23
			76	112	63	28
			93	140	67	24
			92	91	68	25
Test Chemical	Diallyl phthalate	60	67	23	131	65	45*
			107	31	100	31
			72	29	86	40
		80	60	9	237	132	110*
			76	11	222	98
			55	10	166	101
		90					102*
			76	10	282	124
			76	11	183	81
		100					125*
			80	7	274	115
			44	5	178	135
		110

		120


Positive Control	Methyl Methane Sulfonate	5	75	70	372	165	166*
			78	63	355	152
			65	62	359	183

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	76	92	132	58	52
			85	104	114	45
			82	111	111	45
			79	93	141	60
Test Chemical	Diallyl phthalate	12.5	30	24	189	210	120*
			79	68	188	80
			66	62	139	70
		25	85	77	181	71	67
			69	76	143	69
			87	68	157	60
		50	75	51	166	74	80*
			58	39	152	88
			95	56	220	78
		75	83	51	247	100	109*
			61	46	212	116
			69	62	229	111
		100	77	37	327	141	126*
			85	42	302	119
			95	44	335	118
		150	42	13	455	360	323*
			59	12	577	328
			60	15	508	281
Positive Control	3-Methylcholanthrene	2.5	47	34	712	505	557*
			34	29	562	551
			33	21	601	616

Induced S9 Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	69	12	85	41	30
			111	141	56	17
			110	109	99	30
			87	138	85	33
Test Chemical	Diallyl phthalate	25	94	69	87	31	36
			89	67	111	41
			83	60	88	35
		50	88	39	57	22	19
			84	36	33	13
			64	30	45	24
		75	57	17	79	46	42
			58	19	76	43
			52	13	55	36
		100	56	10	78	47	58*
			48	7	79	55
			29	4	62	72
		150


Positive Control	3-Methylcholanthrene	2.5	41	18	278	226	233*
			63	30	337	177
			63	29	559	294

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	40r	68	187	155	90
			44r	96	230	174
			58	105	188	107
			64	95	138	72
Test Chemical	Diallyl phthalate	12.5	81	124	119	49	64
			58	98	119	68
			50	99	113	75
		25	60	119	180	99	100
			66	95	222	112
			65	74	169	87
		50	51#	69	171	113	124
			47	60	192	135
			49	53	185	125
		75	56	53	253	151	173*
			49	49	224	152
			48	36	313	216
		100	53	35	388	244	204*
			53	30	368	233
			52	36	212	136
		150	28	9	440	530	789*
			19	3	587	1048
			9r	2	423	1659
		200


Positive Control	3-Methylcholanthrene	2.5					1156*
			29	17	762	881
			17	8	737	1431

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination