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Study Information
Study Attribute Value
CEBS Accession Number: 002-01987-0008-0000-5
Chemical Name: Diarylanilide yellow
CASRN: 6358-85-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 566314
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 93 80 31 31
    88 106 76 29
    74 106 66 30
    98 95 104 35
Test Chemical Diarylanilide yellow 
 0.0312    
 104 91 99 32 36
    85 89 104 41
  
 0.0625    
 97 82 122 42 40
    87 89 102 39
  
 0.125     
 89 81 124 46 44
    87 68 110 42
  
 0.25      
 87 77 101 39 40
    85 77 108 42
  
 0.5       
 77 73 97 42 46
    97 85 147 50
Positive Control Methyl Methane Sulfonate 
15         
 40 28 149 125 110*
    52 33 147 94
Methyl Methane Sulfonate 
250         
 75 65 411 183 162*
    111 81 467 141

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 110 109 34 30
    101 92 87 29
    92 112 66 24
    106 86 107 34
Test Chemical Diarylanilide yellow 
 0.1       
 90 105 80 30 30
    94 105 87 31
  
 0.2       
 79 103 74 31 30
    79 100 69 29
  
 0.3       
 73 81 99 45 45
    74 85 100 45
  
 0.4       
 76 102 83 36 38
    76 91 89 39
  
 0.5       
 74 92 77 35 37
    74 91 88 40
Positive Control Methyl Methane Sulfonate 
20         
 17 7 151 299 286*
    26 9 214 273
Methyl Methane Sulfonate 
250         
 71 70 630 297 285*
    61 58 499 272

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 105 79 34 30
    66 94 54 27
    66 89 53 27
    92 111 91 33
Test Chemical Diarylanilide yellow 
 0.1       
 80 105 54 23 25
    72 88 59 27
  
 0.2       
 83 133 168 67 61*
    85 133 137 54
  
 0.3       
 95 106 191 67 68*
    84 111 173 69
  
 0.4       
 80 130 192 80 69*
    91 115 160 59
  
 0.5       
 69 97 143 69 62*
    86 99 145 56
Positive Control 3-Methylcholanthrene 
2.5       
 18 5 170 312 286*
    26 6 200 260

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85# 99 91 36 54
    79 83 137 58
    74 102 139 62
    87 116 157 60
Test Chemical Diarylanilide yellow 
 0.1       
 73 81 130 59 48
    76 86 84 37
  
 0.2       
 75 71 152 68 57
    76 86 106 46
  
 0.3       
 84 90 172 68 62
    67 75 114 57
  
 0.4       
 74 76 156 70 61
    77 90 121 53
  
 0.5       
 82 95 119 48 60
    75 65 161 71
Positive Control 3-Methylcholanthrene 
2.5       
 54 19 440 272 307*
    45 23 462 342

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 56 107 135 81 71
    66 95 143 72
    80 107 178 74
    60# 91 102 57
Test Chemical Diarylanilide yellow 
 0.1       
 65 114 148 76 79
    51 119 125 81
  
 0.2       
 73 89 132 60 68
    81 81 181 75
  
 0.3       
 66 78 129 65 76
    55 67 142 86
  
 0.4       
 58 57 196 114 94
    65 78 146 74
  
 0.5       
 69 105 165 80 88
    42 52 120 96
Positive Control 3-Methylcholanthrene 
2.5       
 25 23 701 941 880*
    26 21 651 819

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination