Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01987-0008-0000-5
Chemical Name:	Diarylanilide yellow
CASRN:	6358-85-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	566314
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	93	80	31	31
			88	106	76	29
			74	106	66	30
			98	95	104	35
Test Chemical	Diarylanilide yellow	0.0312	104	91	99	32	36
			85	89	104	41	36
		0.0625	97	82	122	42	40
			87	89	102	39	40
		0.125	89	81	124	46	44
			87	68	110	42	44
		0.25	87	77	101	39	40
			85	77	108	42	40
		0.5	77	73	97	42	46
			97	85	147	50	46
Positive Control	Methyl Methane Sulfonate	15	40	28	149	125	110*
			52	33	147	94	110*
	Methyl Methane Sulfonate	250	75	65	411	183	162*
			111	81	467	141	162*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106	110	109	34	30
			101	92	87	29
			92	112	66	24
			106	86	107	34
Test Chemical	Diarylanilide yellow	0.1	90	105	80	30	30
			94	105	87	31	30
		0.2	79	103	74	31	30
			79	100	69	29	30
		0.3	73	81	99	45	45
			74	85	100	45	45
		0.4	76	102	83	36	38
			76	91	89	39	38
		0.5	74	92	77	35	37
			74	91	88	40	37
Positive Control	Methyl Methane Sulfonate	20	17	7	151	299	286*
			26	9	214	273	286*
	Methyl Methane Sulfonate	250	71	70	630	297	285*
			61	58	499	272	285*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	105	79	34	30
			66	94	54	27
			66	89	53	27
			92	111	91	33
Test Chemical	Diarylanilide yellow	0.1	80	105	54	23	25
			72	88	59	27	25
		0.2	83	133	168	67	61*
			85	133	137	54	61*
		0.3	95	106	191	67	68*
			84	111	173	69	68*
		0.4	80	130	192	80	69*
			91	115	160	59	69*
		0.5	69	97	143	69	62*
			86	99	145	56	62*
Positive Control	3-Methylcholanthrene	2.5	18	5	170	312	286*
Positive Control			26	6	200	260	286*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85#	99	91	36	54
			79	83	137	58
			74	102	139	62
			87	116	157	60
Test Chemical	Diarylanilide yellow	0.1	73	81	130	59	48
			76	86	84	37	48
		0.2	75	71	152	68	57
			76	86	106	46	57
		0.3	84	90	172	68	62
			67	75	114	57	62
		0.4	74	76	156	70	61
			77	90	121	53	61
		0.5	82	95	119	48	60
			75	65	161	71	60
Positive Control	3-Methylcholanthrene	2.5	54	19	440	272	307*
Positive Control			45	23	462	342	307*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	56	107	135	81	71
			66	95	143	72
			80	107	178	74
			60#	91	102	57
Test Chemical	Diarylanilide yellow	0.1	65	114	148	76	79
			51	119	125	81	79
		0.2	73	89	132	60	68
			81	81	181	75	68
		0.3	66	78	129	65	76
			55	67	142	86	76
		0.4	58	57	196	114	94
			65	78	146	74	94
		0.5	69	105	165	80	88
			42	52	120	96	88
Positive Control	3-Methylcholanthrene	2.5	25	23	701	941	880*
Positive Control			26	21	651	819	880*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination