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Study Information
Study Attribute Value
CEBS Accession Number: 002-01988-0001-0000-9
Chemical Name: Diazepam
CASRN: 439-14-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 048662_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 08/10/1989    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Acetone 
0         
 50 1044 434 0.416 8.68 26.0 -10.627
Acetone 
0         
 50 1047 487 0.465 9.74 31.0 0.000
Test Chemical Diazepam 
1.7       
 50 1045 467 0.447 9.34 26.0 -3.923 
5.6       
 50 1046 538 0.514 10.76 26.0 10.578 
17         
 50 1042 482 0.463 9.64 31.0 -0.552 
56         
 0 0 0 0.000 0.00 0.0 N/A 
167         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1046 466 0.446 9.32 26.0 -4.221
Mitomycin-C 
 0.004     
 10 209 186 0.890 18.60 26.0 91.331
Trend: 0.660        
Probability: 0.255        

Trial #: 2    Activation: No Activation    Date: 08/22/1989    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Acetone 
0         
 50 1049 464 0.442 9.28 26.0 -3.551
Acetone 
0         
 50 1051 482 0.459 9.64 31.0 0.000
Test Chemical Diazepam 
1.7       
 50 1045 416 0.398 8.32 26.0 -13.197 
5         
 50 1048 444 0.424 8.88 31.0 -7.620 
17         
 50 1046 400 0.382 8.00 31.0 -16.616 
50         
 0 0 0 0.000 0.00 0.0 N/A 
167         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1046 606 0.579 12.12 26.0 26.327
Mitomycin-C 
 0.004     
 10 211 185 0.877 18.50 26.0 91.181
Trend: -2.306        
Probability: 0.989        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 08/10/1989    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 423 0.403 8.46 26.0 -4.481
Acetone 
0         
 50 1047 442 0.422 8.84 26.0 0.000
Test Chemical Diazepam 
5.6       
 50 1048 462 0.441 9.24 26.0 4.425 
17         
 50 1048 447 0.427 8.94 26.0 1.035 
56         
 50 1049 515 0.491 10.30 26.0 16.294 
167         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.125     
 50 1047 589 0.563 11.78 26.0 33.258
Cyclophosphamide 
 0.5       
 10 210 202 0.962 20.20 26.0 127.854
Trend: 2.108        
Probability: 0.018