Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01989-0008-0000-7
Chemical Name:	Diazinon
CASRN:	333-41-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	180250
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	95	174	79	63
			91	115	147	54
			79	90	135	57
			74	99	134	60
Test Chemical	Diazinon	6.25	72	99	126	58	66
			53	96	115	73	66
		12.5	63	78	131	69	62
			55	80	91	55	62
		25	42	58	128	101	92
			62	71	154	83	92
		50	79	65	194	82	81
			91	61	222	81	81
		100	39	9	202	173	209*
			37	6	273	245	209*
		200

Positive Control	Methyl Methane Sulfonate	15	34	28	427	425	378*
Positive Control			39	25	390	331	378*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	97	203	102	77
			90	100	161	59
			75	98	152	68
			79	105	188	79
Test Chemical	Diazinon	20	82	98	236	96	95
			80	102	225	93	95
		40	83	80	231	93	83
			69	77	148	72	83
		60	69	62	396	193	180*
			79	72	397	168	180*
		80	67	19	2454	1224	1313*
			60	19	2532	1403	1313*
		100

Positive Control	Methyl Methane Sulfonate	15	32	27	95	99	115
Positive Control			30	36	116	130	115

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination