Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02002-0032-0000-1
Chemical Name:	Dibutyl phthalate
CASRN:	84-74-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	800748
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	62	102	69	37	32
			77	88	51	22
			72	111	65	30
			71	99	81	38
Test Chemical	Dibutyl phthalate	12	78	86	58	25	24
			71	79	50	24	24
		24	61	68	67	37	31
			62	77	45	24	31
		36	61	61	47	26	32
			68#	63	79.5	39	32
		48	48#	16	73	51	61*
			38	11	82	72	61*
		60

Positive Control	Methyl Methane Sulfonate	15	34	27	217	212	195*
Positive Control			37	28	198	178	195*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	128	19	8	12
			93	89	30	11
			75#	94	36	16
			87	89	34	13
Test Chemical	Dibutyl phthalate	30	71	44	43	20	20
			88	47	50	19	20
		38	73#	36	31.5	14	18
			64	36	41	21	18
		46	71#	37	76.5	36	32*
			62	25	51	28	32*
		54	66	23	161	82	69*
			69#	19	117	56	69*
		62	66	5	640	323	242*
			78#	8	374	161	242*
		70

Positive Control	Methyl Methane Sulfonate	15	41	22	133	109	101*
Positive Control			29#	17	81	92	101*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	101	90	29	23
			94	101	63	22
			88#	98	46.5	18
			113	101	84	25
Test Chemical	Dibutyl phthalate	38	75	62	73	32	29
			89	67	70	26	29
		46	69	41	81	39	38
			63	34	71	37	38
		54	55	19	78	47	61*
			58	11	130	74	61*
		62	29	4	110	127	126*
			42	4	157	124	126*
		70	18	1	164	307	252*
			17#	1	98	198	252*
Positive Control	Methyl Methane Sulfonate	15	39	23	227	197	205*
Positive Control			36	22	229	213	205*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination