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Study Information
Study Attribute Value
CEBS Accession Number: 002-02002-0032-0000-1
Chemical Name: Dibutyl phthalate
CASRN: 84-74-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 800748
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 62 102 69 37 32
    77 88 51 22
    72 111 65 30
    71 99 81 38
Test Chemical Dibutyl phthalate 
12         
 78 86 58 25 24
    71 79 50 24
  
24         
 61 68 67 37 31
    62 77 45 24
  
36         
 61 61 47 26 32
    68# 63 79.5 39
  
48         
 48# 16 73 51 61*
    38 11 82 72
  
60         
   
Positive Control Methyl Methane Sulfonate 
15         
 34 27 217 212 195*
    37 28 198 178

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 128 19 8 12
    93 89 30 11
    75# 94 36 16
    87 89 34 13
Test Chemical Dibutyl phthalate 
30         
 71 44 43 20 20
    88 47 50 19
  
38         
 73# 36 31.5 14 18
    64 36 41 21
  
46         
 71# 37 76.5 36 32*
    62 25 51 28
  
54         
 66 23 161 82 69*
    69# 19 117 56
  
62         
 66 5 640 323 242*
    78# 8 374 161
  
70         
   
Positive Control Methyl Methane Sulfonate 
15         
 41 22 133 109 101*
    29# 17 81 92

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 101 90 29 23
    94 101 63 22
    88# 98 46.5 18
    113 101 84 25
Test Chemical Dibutyl phthalate 
38         
 75 62 73 32 29
    89 67 70 26
  
46         
 69 41 81 39 38
    63 34 71 37
  
54         
 55 19 78 47 61*
    58 11 130 74
  
62         
 29 4 110 127 126*
    42 4 157 124
  
70         
 18 1 164 307 252*
    17# 1 98 198
Positive Control Methyl Methane Sulfonate 
15         
 39 23 227 197 205*
    36 22 229 213

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination