Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02003-0007-0000-4
Chemical Name:	Dibutyltin diacetate
CASRN:	1067-33-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	881018
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	96	106	80	28	25
			95	97	56	20
			74	97	63	29
Test Chemical	Dibutyltin diacetate	0.02	66#	56	68	34	33
			71#	68	79	37
			51	53	41	27
		0.03	91#	91	53	19	20
			85	90	53	21	20
		0.04	71#	87	60	28	27
			85	79	67	26
			99	97	79	27
		0.05	76	77	62	27	24
			103	74	64	21
			93	80	69	25
		0.06	88#	67	61	23	30
			74	68	66	30
			74	53	82	37
		0.08	81	83	70	29	29
			81	67	57	24
			62	44	67	36
Positive Control	Methyl Methane Sulfonate	5	72	65	306	142	175*
			70#	66	376	179
			66#	49	401	204

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	69	91	85	41	41
			39r	57	63	54
			58	109	72	41
			44r	66	68	52
Test Chemical	Dibutyltin diacetate	0.02	41	40	49	40	51
			33	24	60	61	51
		0.03	58	52	64	37	34
			43	44	61	47
			49	53	25	17
		0.04	59#	42	94	53	80*
			22	18	69	105
			48	27	118	82
		0.05	51	27	188	123	123*
			68#	37	181	89
			35	16	164	156
		0.06


Positive Control	Methyl Methane Sulfonate	5	38	43	260	229	216*
			60#	61	331	183
			51	52	364	237

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	77#	124	98	43	42
			77	95	71	31
			52	56	68	43
			62	125	92	50
Test Chemical	Dibutyltin diacetate	0.02	64	87	65	34	34
			65	104	57	29
			79	120	93	39
		0.03	69	70	115	56	55
			86	96	116	45
			59	79	111	63
		0.04	61	51	144	79	58
			60	60	71	40
			70	48	114	54
		0.05	74	41	299	135	191*
			54	30	440	272
			58	40	289	165
		0.06	45#	13	594	440	438*
			42	12	528	421
			52	14	707	453
		0.08


Positive Control	Methyl Methane Sulfonate	5	52	61	375	240	371*
			34	33	462	455
			39	42	493	418

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination