Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02010-0008-0000-3
Chemical Name:	Dichlorodiphenyltrichloroethane (DDT)
CASRN:	50-29-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	836226
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	115	62	23	24
Vehicle Control			97	85	72	25	24
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	71#	50	34.5	16	18
			68	45	39	19	18
		20	71	36	58	27	28
			85	40	70	27
			73	29	64	29
		30	60	19	54	30	25
			91	35	76	28
			72	24	35	16
		40	96	33	99	34	29
			70	12	55	26
			83#	29	67	27
		50	79	14	73	31	31
			70	7	67	32	31
		60
			72#	3	75	35
		80


Positive Control	Ethyl Methane Sulfonate	250	82	50	1302	531	531*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86#	102	51	20	25
			92	92	85	31
			81	103	82	34
			92	104	45	16
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	65	77	28	14	28
			73	69	93	42	28
		20	69	46	77	37	29
			77	51	48	21
			71	45	59	28
		30	53	29	30	19	16
			67	22	34	17
			80#	32	33	14
		40	64#	16	58.5	31	23
			68	26	36	18
			65	26	39	20
		50	74	20	82	37	36
			75#	25	79.5	36	36
		60	67	16	74	37	53*
			63	19	79	42
			59	7	140	79
		80


Positive Control	Ethyl Methane Sulfonate	250	35#	31	777	744	608*
			50	38	807	543
			56#	41	909	538

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	108	134	53	57
			82	102	165	67
			122r	190	143	39
			96	91	151	52
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	91	71	160	59	64
			106#	48	210	66
			82	45	162	66
		20	86	36	203	79	74
			98	34	207	71
			95	36	208	73
		30	92	30	162	59	68
			103	33	214	69
			96	29	222	77
		40	99	29	186	63	59
			103	26	198	64
			103	28	156	51
		50	158r	28	234	49	61
			96	15	194	67
			87	18	144	55
		60	100	4	284	94	79
			98	14	189	64	79
Positive Control	Ethyl Methane Sulfonate	250	94	64	1205	430	582*
			62	40	1191	642
			56	33	1135	674

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	108	97	38	40
			82	102	120	49
			122r	190	96	26
			96	91	96	33
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	82	45	113	46	43
			106#	48	134	42
			91	71	114	42
		20	86	36	149	58	53
			98	34	145	49
			95	36	150	53
		30	92	30	112	41	48
			103	33	143	46
			96	29	163	57
		40	99	29	131	44	42
			103	26	139	45
			103	28	111	36
		50	158r	28	155	33	40
			96	15	123	43
			87	18	95	37
		60	100	4	183	61	55
			98	14	146	50	55
Positive Control	Ethyl Methane Sulfonate	250	62	40	1051	567	507*
			56	33	972	577
			94	64	1059	378

Non-Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	101	138	49	44
Vehicle Control			110	99	131	40	44
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	99	26	113	38	36
			97	36	99	34	36
		20	101	17	94	31	34
			83	17	92	37	34
		30	90	8	108	40	36
			81	6	77	32
			75	10	83	37
		40	82r	4	72	29

		50


Positive Control	3-Methylcholanthrene	3.5	87	54	501	192	182*
Positive Control			101#	56	525	173	182*

Non-Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	108	297	120	118
			93#	89	372	133
			99#	103	298.5	101
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	102#	33	345	113	120
			79#	32	326	138
			101	30	329	109
		20	74#	22	273	123	90
			78#	19	181.5	78
			81	19	168	69
		30	94#	12	232.5	83	83
			70#	13	171	81
			73#	10	186	85
		40					90
			64#	8	190	99
			70	5	169	81
		50


Positive Control	3-Methylcholanthrene	3.5	98#	83	879	301	317*
			77	73	751	324
			80#	73	778.5	326

Non-Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93#	89	259	93	82
			99#	103	202.5	68
			83	108	215	87
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	10	101	30	204	67	80
			79#	32	229	97
			102#	33	235.5	77
		20	81	19	114	47	65
			74#	22	193.5	87
			78#	19	144	62
		30	73#	10	141	65	62
			70#	13	123	59
			94#	12	177	63
		40					68
			64#	8	137	71
			70	5	136	65
		50


Positive Control	3-Methylcholanthrene	3.5	77	73	475	205	200*
			98#	83	557	190
			80#	73	489	205

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	126r	108	69	18	24
			110	118	79	24
			115#	110	78	23
			116#	72	89	26
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	20	97	24	113	39	38
			79	10	86	36	38
		30	91#	11	84	31	38
			83	11	113	46	38
		40	89	13	97	36	32
			103#	10	85	27	32
		50	97	10	77	26	33
			111	15	105	32
			85#	8	102	40
		60	85	7	137	54	44*
			87	7	91	35	44*
Positive Control	3-Methylcholanthrene	2.5	103	51	502	162	157*
Positive Control			79	56	358	151	157*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65#	54	91.5	47	33
			84	69	101	40
			108#	145	78	24
			90	131	57	21
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	5	68	75	52	25	24
			78	88	50	21
			83	89	62	25
		10	108#	71	45	14	23
			74	54	66	30
			82	59	65	27
		20	78#	37	106.5	46	45
			69	34	92	44
			123r	55	54	15
		40	117#	36	90	26	35
			68	24	96	47
			77#	25	78	34
		50	84	19	121	48	44
			73	18	113	51
			65	14	63	32
		60	46	7	88	64	59*
			66	12	100	51
			59	10	108	61
Positive Control	3-Methylcholanthrene	2.5	85	38	545	213	244*
			46	22	516	374
			104#	56	451	144

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113	106	93	28	22
			96	99	70	24
			89	100	65	24
			108#	95	34.5	11
Test Chemical	Dichlorodiphenyltrichloroethane (DDT)	5	101	46	71	23	23
			103	42	76	25
			83	47	50	20
		10	95	26	79	28	23
			109	34	68	21
			99	32	62	21
		20	75	20	52	23	22
			77	17	45	20
			95	23	68	24
		40	99	20	99	33	33
			86	17	89	35
			88	20	78	30
		50	87	13	93	36	33
			86	12	97	38
			75	15	60	27
		60	88	12	74	28	31
			98	12	91	31
			98	16	104	35
Positive Control	3-Methylcholanthrene	2.5	89#	81	337.5	126	157*
			86	70	413	160
			90	59	500	185

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination