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Study Information
Study Attribute Value
CEBS Accession Number: 002-02014-0009-0000-8
Chemical Name: Dicofol
CASRN: 115-32-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 527222
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96# 104 139.5 48 53
    91 96 155 57
Test Chemical Dicofol 
1.56      
 80 48 172 72 72
    77 53 167 72
    75# 49 162 72
  
3.13      
 87 74 165 63 80*
    68# 59 217 107
    107# 68 226 71
  
6.25      
 93 76 159 57 74
    87 61 193 74
    81# 54 223.5 92
  
12.5       
 107# 67 240 75 83*
    70 69 202 96
    102 69 239 78
  
25         
 102 60 220 72 88*
    78 47 219 94
    80# 51 235.5 98
  
50         
 99 14 227 76 89*
    70 4 228 109
    95# 10 229.5 81
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 60# 34 1154 641 597*
    65# 43 1078.5 553

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 99 121 45 40
    87# 97 101 39
    96# 91 117 41
    85 113 93 36
Test Chemical Dicofol 
5         
 70# 65 59 28 45
    77# 37 116 50
    76 50 131 58
  
10         
 81# 45 108 45 47
    80 38 116 49
    81# 41 119 49
  
20         
 70 24 96 46 56
    60 19 118 66
  
30         
 84 19 135 54 65*
    60 15 141 78
    77 13 144 62
  
40         
    59 8 137 77
   
  
50         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 51 25 897 590 569*
    44 26 718 548

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 121 94 32 38
    81 102 87 36
    65 78 93 48
    95 99 106 37
Test Chemical Dicofol 
5         
 53 33 83 52 51
    69 37 95 46
    45 30 73 54
  
10         
 89 22 132 50 49
    66 25 78 40
    68 19 121 59
  
20         
 72 11 103 48 64*
    55 9 121 73
    47 8 101 72
  
30         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 68 65 830 405 455*
    63 54 908 480
    64# 60 919 481

Non-Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 108 94 31 32
    78# 86 74 32
    114 121 115 34
    78# 86 71 30
Test Chemical Dicofol 
5         
 95 75 92 32 35
    79 67 81 34
    87 57 102 39
  
10         
 104 65 125 40 36
    101# 75 107 35
    89 74 91 34
  
20         
 99 55 113 38 36
    85 47 89 35
    98# 41 105 36
  
30         
 85 39 115 45 46
    89 43 126 47
  
40         
 80 28 132 55 51*
    95# 32 134 47
  
50         
 84# 29 84 34 37
    94 33 117 41
Positive Control 3-Methylcholanthrene 
3.5       
 104# 66 351 112 130*
    104# 53 444 142
    96# 64 387 135

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 107 120 233 72 73
    90# 94 210 78
    105# 86 212 67
Test Chemical Dicofol 
1.56      
 77 56 117 51 53
    83 67 137 55
  
3.13      
 109 56 150 46 52
    83 27 167 67
    101 40 135 45
  
6.25      
 93# 66 137 49 59
    87 38 150 57
    60# 27 124.5 69
  
12.5       
 126r 77 230 61
    132r 81 232 59
    100 87 192 64
  
25         
 110 89 211 64 60
    115 73 193 56
    109 66 192 59
  
50         
 108 61 159 49 64
    95 22 185 65
    79 26 183 77
  
100         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 92# 69 538.5 195 192*
    95# 63 537 188

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 109 74 27 24
    110 112 82 25
    62 80 40 22
Test Chemical Dicofol 
10         
 73 27 85 39 33
    91 62 79 29
    74 45 68 31
  
20         
 74 47 76 34 40*
    86 34 121 47
    73 43 86 40
  
30         
 74 17 96 43 41*
    71 16 90 42
    86 30 101 39
  
40         
 68 24 93 45 41*
    63 31 76 40
    80 35 86 36
  
50         
 87 25 86 33 40*
    59 18 82 46
    71 31 85 40
  
60         
 82# 20 109 44 38*
    74# 22 72 32
    87 29 99 38
Positive Control 3-Methylcholanthrene 
2.5       
 89 56 615 230 210*
    100 86 541 180
    91 56 603 220

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination