Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02014-0009-0000-8
Chemical Name:	Dicofol
CASRN:	115-32-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	527222
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96#	104	139.5	48	53
Vehicle Control			91	96	155	57	53
Test Chemical	Dicofol	1.56	80	48	172	72	72
			77	53	167	72
			75#	49	162	72
		3.13	87	74	165	63	80*
			68#	59	217	107
			107#	68	226	71
		6.25	93	76	159	57	74
			87	61	193	74
			81#	54	223.5	92
		12.5	107#	67	240	75	83*
			70	69	202	96
			102	69	239	78
		25	102	60	220	72	88*
			78	47	219	94
			80#	51	235.5	98
		50	99	14	227	76	89*
			70	4	228	109
			95#	10	229.5	81
		100


Positive Control	Ethyl Methane Sulfonate	250	60#	34	1154	641	597*
Positive Control			65#	43	1078.5	553	597*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	99	121	45	40
			87#	97	101	39
			96#	91	117	41
			85	113	93	36
Test Chemical	Dicofol	5	70#	65	59	28	45
			77#	37	116	50
			76	50	131	58
		10	81#	45	108	45	47
			80	38	116	49
			81#	41	119	49
		20	70	24	96	46	56
			60	19	118	66	56
		30	84	19	135	54	65*
			60	15	141	78
			77	13	144	62
		40
			59	8	137	77

		50


Positive Control	Ethyl Methane Sulfonate	250	51	25	897	590	569*
Positive Control			44	26	718	548	569*

Nonactivation Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	121	94	32	38
			81	102	87	36
			65	78	93	48
			95	99	106	37
Test Chemical	Dicofol	5	53	33	83	52	51
			69	37	95	46
			45	30	73	54
		10	89	22	132	50	49
			66	25	78	40
			68	19	121	59
		20	72	11	103	48	64*
			55	9	121	73
			47	8	101	72
		30


Positive Control	Ethyl Methane Sulfonate	250	68	65	830	405	455*
			63	54	908	480
			64#	60	919	481

Non-Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	108	94	31	32
			78#	86	74	32
			114	121	115	34
			78#	86	71	30
Test Chemical	Dicofol	5	95	75	92	32	35
			79	67	81	34
			87	57	102	39
		10	104	65	125	40	36
			101#	75	107	35
			89	74	91	34
		20	99	55	113	38	36
			85	47	89	35
			98#	41	105	36
		30	85	39	115	45	46
			89	43	126	47	46
		40	80	28	132	55	51*
			95#	32	134	47	51*
		50	84#	29	84	34	37
			94	33	117	41	37
Positive Control	3-Methylcholanthrene	3.5	104#	66	351	112	130*
			104#	53	444	142
			96#	64	387	135

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	107	120	233	72	73
			90#	94	210	78
			105#	86	212	67
Test Chemical	Dicofol	1.56	77	56	117	51	53
			83	67	137	55	53
		3.13	109	56	150	46	52
			83	27	167	67
			101	40	135	45
		6.25	93#	66	137	49	59
			87	38	150	57
			60#	27	124.5	69
		12.5	126r	77	230	61
			132r	81	232	59
			100	87	192	64
		25	110	89	211	64	60
			115	73	193	56
			109	66	192	59
		50	108	61	159	49	64
			95	22	185	65
			79	26	183	77
		100


Positive Control	3-Methylcholanthrene	2.5	92#	69	538.5	195	192*
Positive Control			95#	63	537	188	192*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	109	74	27	24
			110	112	82	25
			62	80	40	22
Test Chemical	Dicofol	10	73	27	85	39	33
			91	62	79	29
			74	45	68	31
		20	74	47	76	34	40*
			86	34	121	47
			73	43	86	40
		30	74	17	96	43	41*
			71	16	90	42
			86	30	101	39
		40	68	24	93	45	41*
			63	31	76	40
			80	35	86	36
		50	87	25	86	33	40*
			59	18	82	46
			71	31	85	40
		60	82#	20	109	44	38*
			74#	22	72	32
			87	29	99	38
Positive Control	3-Methylcholanthrene	2.5	89	56	615	230	210*
			100	86	541	180
			91	56	603	220

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination